A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections
Abstract Background The complex balance between environmental and host factors is an important determinant of susceptibility to infection. Disturbances of this equilibrium may result in multifactorial diseases as illustrated by the summer mortality syndrome, a worldwide and complex phenomenon that a...
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crspringernat:10.1186/1471-2164-13-252 2023-05-15T15:59:01+02:00 A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections Rosa, Rafael Diego de Lorgeril, Julien Tailliez, Patrick Bruno, Roman Piquemal, David Bachère, Evelyne 2012 http://dx.doi.org/10.1186/1471-2164-13-252 https://link.springer.com/content/pdf/10.1186/1471-2164-13-252.pdf en eng Springer Science and Business Media LLC BMC Genomics volume 13, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2012 crspringernat https://doi.org/10.1186/1471-2164-13-252 2022-01-04T09:16:43Z Abstract Background The complex balance between environmental and host factors is an important determinant of susceptibility to infection. Disturbances of this equilibrium may result in multifactorial diseases as illustrated by the summer mortality syndrome, a worldwide and complex phenomenon that affects the oysters, Crassostrea gigas. The summer mortality syndrome reveals a physiological intolerance making this oyster species susceptible to diseases. Exploration of genetic basis governing the oyster resistance or susceptibility to infections is thus a major goal for understanding field mortality events. In this context, we used high-throughput genomic approaches to identify genetic traits that may characterize inherent survival capacities in C. gigas . Results Using digital gene expression (DGE), we analyzed the transcriptomes of hemocytes (immunocompetent cells) of oysters able or not able to survive infections by Vibrio species shown to be involved in summer mortalities. Hemocytes were nonlethally collected from oysters before Vibrio experimental infection, and two DGE libraries were generated from individuals that survived or did not survive. Exploration of DGE data and microfluidic qPCR analyses at individual level showed an extraordinary polymorphism in gene expressions, but also a set of hemocyte-expressed genes whose basal mRNA levels discriminate oyster capacity to survive infections by the pathogenic V. splendidus LGP32. Finally, we identified a signature of 14 genes that predicted oyster survival capacity. Their expressions are likely driven by distinct transcriptional regulation processes associated or not associated to gene copy number variation (CNV). Conclusions We provide here for the first time in oyster a gene expression survival signature that represents a useful tool for understanding mortality events and for assessing genetic traits of interest for disease resistance selection programs. Article in Journal/Newspaper Crassostrea gigas Springer Nature (via Crossref) BMC Genomics 13 1 |
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English |
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Genetics Biotechnology |
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Genetics Biotechnology Rosa, Rafael Diego de Lorgeril, Julien Tailliez, Patrick Bruno, Roman Piquemal, David Bachère, Evelyne A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background The complex balance between environmental and host factors is an important determinant of susceptibility to infection. Disturbances of this equilibrium may result in multifactorial diseases as illustrated by the summer mortality syndrome, a worldwide and complex phenomenon that affects the oysters, Crassostrea gigas. The summer mortality syndrome reveals a physiological intolerance making this oyster species susceptible to diseases. Exploration of genetic basis governing the oyster resistance or susceptibility to infections is thus a major goal for understanding field mortality events. In this context, we used high-throughput genomic approaches to identify genetic traits that may characterize inherent survival capacities in C. gigas . Results Using digital gene expression (DGE), we analyzed the transcriptomes of hemocytes (immunocompetent cells) of oysters able or not able to survive infections by Vibrio species shown to be involved in summer mortalities. Hemocytes were nonlethally collected from oysters before Vibrio experimental infection, and two DGE libraries were generated from individuals that survived or did not survive. Exploration of DGE data and microfluidic qPCR analyses at individual level showed an extraordinary polymorphism in gene expressions, but also a set of hemocyte-expressed genes whose basal mRNA levels discriminate oyster capacity to survive infections by the pathogenic V. splendidus LGP32. Finally, we identified a signature of 14 genes that predicted oyster survival capacity. Their expressions are likely driven by distinct transcriptional regulation processes associated or not associated to gene copy number variation (CNV). Conclusions We provide here for the first time in oyster a gene expression survival signature that represents a useful tool for understanding mortality events and for assessing genetic traits of interest for disease resistance selection programs. |
format |
Article in Journal/Newspaper |
author |
Rosa, Rafael Diego de Lorgeril, Julien Tailliez, Patrick Bruno, Roman Piquemal, David Bachère, Evelyne |
author_facet |
Rosa, Rafael Diego de Lorgeril, Julien Tailliez, Patrick Bruno, Roman Piquemal, David Bachère, Evelyne |
author_sort |
Rosa, Rafael Diego |
title |
A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
title_short |
A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
title_full |
A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
title_fullStr |
A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
title_full_unstemmed |
A hemocyte gene expression signature correlated with predictive capacity of oysters to survive Vibrio infections |
title_sort |
hemocyte gene expression signature correlated with predictive capacity of oysters to survive vibrio infections |
publisher |
Springer Science and Business Media LLC |
publishDate |
2012 |
url |
http://dx.doi.org/10.1186/1471-2164-13-252 https://link.springer.com/content/pdf/10.1186/1471-2164-13-252.pdf |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
BMC Genomics volume 13, issue 1 ISSN 1471-2164 |
op_doi |
https://doi.org/10.1186/1471-2164-13-252 |
container_title |
BMC Genomics |
container_volume |
13 |
container_issue |
1 |
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1766394799310503936 |