Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar
Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportuni...
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crspringernat:10.1186/1471-2164-11-486 2023-05-15T15:28:43+02:00 Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar Yasuike, Motoshige de Boer, Johan von Schalburg, Kristian R Cooper, Glenn A McKinnel, Linda Messmer, Amber So, Stacy Davidson, William S Koop, Ben F 2010 http://dx.doi.org/10.1186/1471-2164-11-486 https://link.springer.com/content/pdf/10.1186/1471-2164-11-486.pdf en eng Springer Science and Business Media LLC BMC Genomics volume 11, issue 1 ISSN 1471-2164 Genetics Biotechnology journal-article 2010 crspringernat https://doi.org/10.1186/1471-2164-11-486 2022-01-04T14:05:36Z Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportunity to examine the mechanisms of genome diversity and genome evolution of the IgH loci in vertebrates. In this study, we defined the structure of these loci in Atlantic salmon, and sequenced 24 bacterial artificial chromosome (BAC) clones that were assembled into the IGH-A (1.1 Mb) and IGH-B (0.9 Mb) loci. In addition, over 7,000 cDNA clones from the IgH variable (V H ) region have been sequenced and analyzed. Results The present study shows that the genomic organization of the duplicated IgH loci in Atlantic salmon differs from that in other teleosts and other vertebrates. The loci possess multiple Cτ genes upstream of the Cμ region, with three of the Cτ genes being functional. Moreover, the duplicated loci possess over 300 V H segments which could be classified into 18 families. This is the largest number of V H families currently defined in any vertebrate. There were significant structural differences between the two loci, indicating that both IGH-A and -B loci have evolved independently in the short time after the recent genome duplication approximately 60 mya. Conclusions Our results indicate that the duplication of the IgH loci in Atlantic salmon significantly contributes to the increased diversity of the antibody repertoire, as compared with the single IgH locus in other vertebrates. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) BMC Genomics 11 1 486 |
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Springer Nature (via Crossref) |
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English |
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Genetics Biotechnology |
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Genetics Biotechnology Yasuike, Motoshige de Boer, Johan von Schalburg, Kristian R Cooper, Glenn A McKinnel, Linda Messmer, Amber So, Stacy Davidson, William S Koop, Ben F Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
topic_facet |
Genetics Biotechnology |
description |
Abstract Background The Atlantic salmon ( Salmo salar ) immunoglobulin heavy chain ( IgH ) locus possesses two parallel IgH isoloci ( IGH-A and IGH-B ), that are related to the genomic duplication event in the family Salmonidae. These duplicated IgH loci in Atlantic salmon provide a unique opportunity to examine the mechanisms of genome diversity and genome evolution of the IgH loci in vertebrates. In this study, we defined the structure of these loci in Atlantic salmon, and sequenced 24 bacterial artificial chromosome (BAC) clones that were assembled into the IGH-A (1.1 Mb) and IGH-B (0.9 Mb) loci. In addition, over 7,000 cDNA clones from the IgH variable (V H ) region have been sequenced and analyzed. Results The present study shows that the genomic organization of the duplicated IgH loci in Atlantic salmon differs from that in other teleosts and other vertebrates. The loci possess multiple Cτ genes upstream of the Cμ region, with three of the Cτ genes being functional. Moreover, the duplicated loci possess over 300 V H segments which could be classified into 18 families. This is the largest number of V H families currently defined in any vertebrate. There were significant structural differences between the two loci, indicating that both IGH-A and -B loci have evolved independently in the short time after the recent genome duplication approximately 60 mya. Conclusions Our results indicate that the duplication of the IgH loci in Atlantic salmon significantly contributes to the increased diversity of the antibody repertoire, as compared with the single IgH locus in other vertebrates. |
format |
Article in Journal/Newspaper |
author |
Yasuike, Motoshige de Boer, Johan von Schalburg, Kristian R Cooper, Glenn A McKinnel, Linda Messmer, Amber So, Stacy Davidson, William S Koop, Ben F |
author_facet |
Yasuike, Motoshige de Boer, Johan von Schalburg, Kristian R Cooper, Glenn A McKinnel, Linda Messmer, Amber So, Stacy Davidson, William S Koop, Ben F |
author_sort |
Yasuike, Motoshige |
title |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_short |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_full |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_fullStr |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_full_unstemmed |
Evolution of duplicated IgH loci in Atlantic salmon, Salmo salar |
title_sort |
evolution of duplicated igh loci in atlantic salmon, salmo salar |
publisher |
Springer Science and Business Media LLC |
publishDate |
2010 |
url |
http://dx.doi.org/10.1186/1471-2164-11-486 https://link.springer.com/content/pdf/10.1186/1471-2164-11-486.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Genomics volume 11, issue 1 ISSN 1471-2164 |
op_doi |
https://doi.org/10.1186/1471-2164-11-486 |
container_title |
BMC Genomics |
container_volume |
11 |
container_issue |
1 |
container_start_page |
486 |
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1766359053444841472 |