Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins
Abstract The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and...
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2021
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Online Access: | http://dx.doi.org/10.1038/s42003-021-02179-x http://www.nature.com/articles/s42003-021-02179-x.pdf http://www.nature.com/articles/s42003-021-02179-x |
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crspringernat:10.1038/s42003-021-02179-x 2023-05-15T18:33:31+02:00 Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve 2021 http://dx.doi.org/10.1038/s42003-021-02179-x http://www.nature.com/articles/s42003-021-02179-x.pdf http://www.nature.com/articles/s42003-021-02179-x en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Communications Biology volume 4, issue 1 ISSN 2399-3642 General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) journal-article 2021 crspringernat https://doi.org/10.1038/s42003-021-02179-x 2022-01-04T16:10:55Z Abstract The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood samples (n = 446) from known age animals representing nine odontocete species within 4 phylogenetic families to identify age associated CG dinucleotides (CpGs). The top CpGs were used to create a cross-validated OEAC clock which was highly correlated for individuals (r = 0.94) and for unique species (median r = 0.93). Finally, we applied the OEAC for estimating the age and sex of 22 wild Norwegian killer whales. DNA methylation patterns of age associated CpGs are highly conserved across odontocetes. These similarities allowed us to develop an odontocete epigenetic aging clock (OEAC) which can be used for species conservation efforts by provide a mechanism for estimating the age of free ranging odontocetes from either blood or skin samples. Article in Journal/Newspaper toothed whales Springer Nature (via Crossref) Communications Biology 4 1 |
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Springer Nature (via Crossref) |
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English |
topic |
General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) |
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General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
topic_facet |
General Agricultural and Biological Sciences General Biochemistry, Genetics and Molecular Biology Medicine (miscellaneous) |
description |
Abstract The development of a precise blood or skin tissue DNA Epigenetic Aging Clock for Odontocete (OEAC) would solve current age estimation inaccuracies for wild odontocetes. Therefore, we determined genome-wide DNA methylation profiles using a custom array (HorvathMammalMethyl40) across skin and blood samples (n = 446) from known age animals representing nine odontocete species within 4 phylogenetic families to identify age associated CG dinucleotides (CpGs). The top CpGs were used to create a cross-validated OEAC clock which was highly correlated for individuals (r = 0.94) and for unique species (median r = 0.93). Finally, we applied the OEAC for estimating the age and sex of 22 wild Norwegian killer whales. DNA methylation patterns of age associated CpGs are highly conserved across odontocetes. These similarities allowed us to develop an odontocete epigenetic aging clock (OEAC) which can be used for species conservation efforts by provide a mechanism for estimating the age of free ranging odontocetes from either blood or skin samples. |
format |
Article in Journal/Newspaper |
author |
Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve |
author_facet |
Robeck, Todd R. Fei, Zhe Lu, Ake T. Haghani, Amin Jourdain, Eve Zoller, Joseph A. Li, Caesar Z. Steinman, Karen J. DiRocco, Stacy Schmitt, Todd Osborn, Steve Van Bonn, Bill Katsumata, Etsuko Mergl, June Almunia, Javier Rodriguez, Magdalena Haulena, Martin Dold, Christopher Horvath, Steve |
author_sort |
Robeck, Todd R. |
title |
Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
title_short |
Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
title_full |
Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
title_fullStr |
Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
title_full_unstemmed |
Multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
title_sort |
multi-species and multi-tissue methylation clocks for age estimation in toothed whales and dolphins |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s42003-021-02179-x http://www.nature.com/articles/s42003-021-02179-x.pdf http://www.nature.com/articles/s42003-021-02179-x |
genre |
toothed whales |
genre_facet |
toothed whales |
op_source |
Communications Biology volume 4, issue 1 ISSN 2399-3642 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s42003-021-02179-x |
container_title |
Communications Biology |
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4 |
container_issue |
1 |
_version_ |
1766218129715757056 |