Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease
Abstract Amoebic Gill Disease (AGD), caused by the protozoan extracellular parasite Paramoeba perurans ( P. perurans ) is a disease affecting Atlantic salmon ( Salmo salar ). This study investigated the gill transcriptomic profile of pre-clinical AGD using RNA-sequencing (RNA-seq) technology. RNA-se...
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2021
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Online Access: | http://dx.doi.org/10.1038/s41598-021-99996-1 https://www.nature.com/articles/s41598-021-99996-1.pdf https://www.nature.com/articles/s41598-021-99996-1 |
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crspringernat:10.1038/s41598-021-99996-1 2023-05-15T15:31:36+02:00 Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease Talbot, Anita Gargan, Laura Moran, Grainne Prudent, Louis O’Connor, Ian Mirimin, Luca Carlsson, Jens MacCarthy, Eugene Department of Agriculture, Food and the Marine, Ireland 2021 http://dx.doi.org/10.1038/s41598-021-99996-1 https://www.nature.com/articles/s41598-021-99996-1.pdf https://www.nature.com/articles/s41598-021-99996-1 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 11, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2021 crspringernat https://doi.org/10.1038/s41598-021-99996-1 2022-01-04T15:13:00Z Abstract Amoebic Gill Disease (AGD), caused by the protozoan extracellular parasite Paramoeba perurans ( P. perurans ) is a disease affecting Atlantic salmon ( Salmo salar ). This study investigated the gill transcriptomic profile of pre-clinical AGD using RNA-sequencing (RNA-seq) technology. RNA-seq libraries generated at 0, 4, 7, 14 and 16 days post infection (dpi) identified 19,251 differentially expressed genes (DEGs) of which 56.2% were up-regulated. DEGs mapped to 224 Gene Ontology (GO) terms including 140 biological processes (BP), 45 cellular components (CC), and 39 molecular functions (MF). A total of 27 reference pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and 15 Reactome gene sets were identified. The RNA-seq data was validated using real-time, quantitative PCR (qPCR). A host immune response though the activation of complement and the acute phase genes was evident at 7 dpi, with a concurrent immune suppression involving cytokine signalling, notably in interleukins, interferon regulatory factors and tumour necrosis factor-alpha ( tnf- α) genes. Down-regulated gene expression with involvement in receptor signalling pathways (NOD-like, Toll-like and RIG-1) were also identified. The results of this study support the theory that P. perurans can evade immune surveillance during the initial stages of gill colonisation through interference of signal transduction pathways. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) Scientific Reports 11 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Multidisciplinary |
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Multidisciplinary Talbot, Anita Gargan, Laura Moran, Grainne Prudent, Louis O’Connor, Ian Mirimin, Luca Carlsson, Jens MacCarthy, Eugene Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
topic_facet |
Multidisciplinary |
description |
Abstract Amoebic Gill Disease (AGD), caused by the protozoan extracellular parasite Paramoeba perurans ( P. perurans ) is a disease affecting Atlantic salmon ( Salmo salar ). This study investigated the gill transcriptomic profile of pre-clinical AGD using RNA-sequencing (RNA-seq) technology. RNA-seq libraries generated at 0, 4, 7, 14 and 16 days post infection (dpi) identified 19,251 differentially expressed genes (DEGs) of which 56.2% were up-regulated. DEGs mapped to 224 Gene Ontology (GO) terms including 140 biological processes (BP), 45 cellular components (CC), and 39 molecular functions (MF). A total of 27 reference pathways in the Kyoto Encyclopedia of Genes and Genomes (KEGG) and 15 Reactome gene sets were identified. The RNA-seq data was validated using real-time, quantitative PCR (qPCR). A host immune response though the activation of complement and the acute phase genes was evident at 7 dpi, with a concurrent immune suppression involving cytokine signalling, notably in interleukins, interferon regulatory factors and tumour necrosis factor-alpha ( tnf- α) genes. Down-regulated gene expression with involvement in receptor signalling pathways (NOD-like, Toll-like and RIG-1) were also identified. The results of this study support the theory that P. perurans can evade immune surveillance during the initial stages of gill colonisation through interference of signal transduction pathways. |
author2 |
Department of Agriculture, Food and the Marine, Ireland |
format |
Article in Journal/Newspaper |
author |
Talbot, Anita Gargan, Laura Moran, Grainne Prudent, Louis O’Connor, Ian Mirimin, Luca Carlsson, Jens MacCarthy, Eugene |
author_facet |
Talbot, Anita Gargan, Laura Moran, Grainne Prudent, Louis O’Connor, Ian Mirimin, Luca Carlsson, Jens MacCarthy, Eugene |
author_sort |
Talbot, Anita |
title |
Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
title_short |
Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
title_full |
Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
title_fullStr |
Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
title_full_unstemmed |
Investigation of the transcriptomic response in Atlantic salmon (Salmo salar) gill exposed to Paramoeba perurans during early onset of disease |
title_sort |
investigation of the transcriptomic response in atlantic salmon (salmo salar) gill exposed to paramoeba perurans during early onset of disease |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s41598-021-99996-1 https://www.nature.com/articles/s41598-021-99996-1.pdf https://www.nature.com/articles/s41598-021-99996-1 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Scientific Reports volume 11, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-021-99996-1 |
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Scientific Reports |
container_volume |
11 |
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1 |
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1766362121750183936 |