Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic
Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N 2 ) fixing marine diazotrophs, knowledge gap...
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crspringernat:10.1038/s41598-021-84969-1 2023-05-15T17:30:25+02:00 Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas National Science Foundation,United States Agence Nationale de la Recherche 2021 http://dx.doi.org/10.1038/s41598-021-84969-1 http://www.nature.com/articles/s41598-021-84969-1.pdf http://www.nature.com/articles/s41598-021-84969-1 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 11, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2021 crspringernat https://doi.org/10.1038/s41598-021-84969-1 2022-01-04T10:13:46Z Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N 2 ) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N 2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N 2 fixation and net community production (NCP). Maximum N 2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae . Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N 2 fixation coastal sites. Article in Journal/Newspaper North Atlantic Springer Nature (via Crossref) Scientific Reports 11 1 |
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Multidisciplinary Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
topic_facet |
Multidisciplinary |
description |
Abstract Variation in the microbial cycling of nutrients and carbon in the ocean is an emergent property of complex planktonic communities. While recent findings have considerably expanded our understanding of the diversity and distribution of nitrogen (N 2 ) fixing marine diazotrophs, knowledge gaps remain regarding ecological interactions between diazotrophs and other community members. Using quantitative 16S and 18S V4 rDNA amplicon sequencing, we surveyed eukaryotic and prokaryotic microbial communities from samples collected in August 2016 and 2017 across the Western North Atlantic. Leveraging and significantly expanding an earlier published 2015 molecular dataset, we examined microbial community structure and ecological co-occurrence relationships associated with intense hotspots of N 2 fixation previously reported at sites off the Southern New England Shelf and Mid-Atlantic Bight. Overall, we observed a negative relationship between eukaryotic diversity and both N 2 fixation and net community production (NCP). Maximum N 2 fixation rates occurred at sites with high abundances of mixotrophic stramenopiles, notably Chrysophyceae . Network analysis revealed such stramenopiles to be keystone taxa alongside the haptophyte diazotroph host Braarudosphaera bigelowii and chlorophytes. Our findings highlight an intriguing relationship between marine stramenopiles and high N 2 fixation coastal sites. |
author2 |
National Science Foundation,United States Agence Nationale de la Recherche |
format |
Article in Journal/Newspaper |
author |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas |
author_facet |
Wang, Seaver Tang, Weiyi Delage, Erwan Gifford, Scott Whitby, Hannah González, Aridane G. Eveillard, Damien Planquette, Hélène Cassar, Nicolas |
author_sort |
Wang, Seaver |
title |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_short |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_fullStr |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_full_unstemmed |
Investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western North Atlantic |
title_sort |
investigating the microbial ecology of coastal hotspots of marine nitrogen fixation in the western north atlantic |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s41598-021-84969-1 http://www.nature.com/articles/s41598-021-84969-1.pdf http://www.nature.com/articles/s41598-021-84969-1 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Scientific Reports volume 11, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-021-84969-1 |
container_title |
Scientific Reports |
container_volume |
11 |
container_issue |
1 |
_version_ |
1766126795648663552 |