Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections
Abstract Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great...
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Online Access: | http://dx.doi.org/10.1038/s41598-021-03638-5 https://www.nature.com/articles/s41598-021-03638-5.pdf https://www.nature.com/articles/s41598-021-03638-5 |
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crspringernat:10.1038/s41598-021-03638-5 2023-05-15T16:23:00+02:00 Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections Störk, Theresa de le Roi, Madeleine Haverkamp, Ann-Kathrin Jesse, Sonja T. Peters, Martin Fast, Christine Gregor, Katharina M. Könenkamp, Laura Steffen, Imke Ludlow, Martin Beineke, Andreas Hansmann, Florian Wohlsein, Peter Osterhaus, Albert D. M. E. Baumgärtner, Wolfgang Deutsche Forschungsgemeinschaft Stiftung Tierärztliche Hochschule Hannover (TIHO) 2021 http://dx.doi.org/10.1038/s41598-021-03638-5 https://www.nature.com/articles/s41598-021-03638-5.pdf https://www.nature.com/articles/s41598-021-03638-5 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 11, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2021 crspringernat https://doi.org/10.1038/s41598-021-03638-5 2022-01-04T09:38:54Z Abstract Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great grey owl, and one kingfisher collected from 2011 to 2018 and positive for USUV by qRT-PCR were investigated. Macroscopically, most USUV infected birds showed splenomegaly and hepatomegaly. Histopathological lesions included necrosis and lymphohistiocytic inflammation within spleen, Bursa fabricii, liver, heart, brain, lung and intestine. Immunohistochemistry revealed USUV antigen positive cells in heart, spleen, pancreas, lung, brain, proventriculus/gizzard, Bursa fabricii, kidney, intestine, skeletal muscle, and liver. Analysis of viral genome allocated the virus to Europe 3 or Africa 2 lineage. This study investigated whether immunohistochemical detection of double-stranded ribonucleic acid (dsRNA) serves as an alternative tool to detect viral intermediates. Tissue samples of six animals with confirmed USUV infection by qRT-PCR but lacking viral antigen in liver and spleen, were further examined immunohistochemically. Two animals exhibited a positive signal for dsRNA. This could indicate either an early state of infection without sufficient formation of virus translation products, occurrence of another concurrent virus infection or endogenous dsRNA not related to infectious pathogens and should be investigated in more detail in future studies. Article in Journal/Newspaper great grey owl Springer Nature (via Crossref) Scientific Reports 11 1 |
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Springer Nature (via Crossref) |
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crspringernat |
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English |
topic |
Multidisciplinary |
spellingShingle |
Multidisciplinary Störk, Theresa de le Roi, Madeleine Haverkamp, Ann-Kathrin Jesse, Sonja T. Peters, Martin Fast, Christine Gregor, Katharina M. Könenkamp, Laura Steffen, Imke Ludlow, Martin Beineke, Andreas Hansmann, Florian Wohlsein, Peter Osterhaus, Albert D. M. E. Baumgärtner, Wolfgang Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
topic_facet |
Multidisciplinary |
description |
Abstract Usutu virus (USUV) is a zoonotic arbovirus causing avian mass mortalities. The first outbreak in North-Western Germany occurred in 2018. This retrospective analysis focused on combining virological and pathological findings in birds and immunohistochemistry. 25 common blackbirds, one great grey owl, and one kingfisher collected from 2011 to 2018 and positive for USUV by qRT-PCR were investigated. Macroscopically, most USUV infected birds showed splenomegaly and hepatomegaly. Histopathological lesions included necrosis and lymphohistiocytic inflammation within spleen, Bursa fabricii, liver, heart, brain, lung and intestine. Immunohistochemistry revealed USUV antigen positive cells in heart, spleen, pancreas, lung, brain, proventriculus/gizzard, Bursa fabricii, kidney, intestine, skeletal muscle, and liver. Analysis of viral genome allocated the virus to Europe 3 or Africa 2 lineage. This study investigated whether immunohistochemical detection of double-stranded ribonucleic acid (dsRNA) serves as an alternative tool to detect viral intermediates. Tissue samples of six animals with confirmed USUV infection by qRT-PCR but lacking viral antigen in liver and spleen, were further examined immunohistochemically. Two animals exhibited a positive signal for dsRNA. This could indicate either an early state of infection without sufficient formation of virus translation products, occurrence of another concurrent virus infection or endogenous dsRNA not related to infectious pathogens and should be investigated in more detail in future studies. |
author2 |
Deutsche Forschungsgemeinschaft Stiftung Tierärztliche Hochschule Hannover (TIHO) |
format |
Article in Journal/Newspaper |
author |
Störk, Theresa de le Roi, Madeleine Haverkamp, Ann-Kathrin Jesse, Sonja T. Peters, Martin Fast, Christine Gregor, Katharina M. Könenkamp, Laura Steffen, Imke Ludlow, Martin Beineke, Andreas Hansmann, Florian Wohlsein, Peter Osterhaus, Albert D. M. E. Baumgärtner, Wolfgang |
author_facet |
Störk, Theresa de le Roi, Madeleine Haverkamp, Ann-Kathrin Jesse, Sonja T. Peters, Martin Fast, Christine Gregor, Katharina M. Könenkamp, Laura Steffen, Imke Ludlow, Martin Beineke, Andreas Hansmann, Florian Wohlsein, Peter Osterhaus, Albert D. M. E. Baumgärtner, Wolfgang |
author_sort |
Störk, Theresa |
title |
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
title_short |
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
title_full |
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
title_fullStr |
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
title_full_unstemmed |
Analysis of avian Usutu virus infections in Germany from 2011 to 2018 with focus on dsRNA detection to demonstrate viral infections |
title_sort |
analysis of avian usutu virus infections in germany from 2011 to 2018 with focus on dsrna detection to demonstrate viral infections |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s41598-021-03638-5 https://www.nature.com/articles/s41598-021-03638-5.pdf https://www.nature.com/articles/s41598-021-03638-5 |
genre |
great grey owl |
genre_facet |
great grey owl |
op_source |
Scientific Reports volume 11, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-021-03638-5 |
container_title |
Scientific Reports |
container_volume |
11 |
container_issue |
1 |
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1766011159084793856 |