Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard
Abstract The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron mi...
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Springer Science and Business Media LLC
2021
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Online Access: | http://dx.doi.org/10.1038/s41598-021-01211-8 https://www.nature.com/articles/s41598-021-01211-8.pdf https://www.nature.com/articles/s41598-021-01211-8 |
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crspringernat:10.1038/s41598-021-01211-8 2023-05-15T15:06:50+02:00 Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn 2021 http://dx.doi.org/10.1038/s41598-021-01211-8 https://www.nature.com/articles/s41598-021-01211-8.pdf https://www.nature.com/articles/s41598-021-01211-8 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 11, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2021 crspringernat https://doi.org/10.1038/s41598-021-01211-8 2022-01-04T10:36:35Z Abstract The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Fluorescence microscopy using Calcofluor white stain supported the observations of parasitic fungi on the algal cells. Images in brightfield microscopy showed chytrid-like fungi penetrating the cells of both algal species. Parasites were found to colonize the cells of A. nordenskioeldii and hypnozygotes of S. nivaloides , while no fungi infected the cyst stages of S. nivaloides . The autofluorescence analysis revealed the ability of S. nivaloides to glow when excited with different wavelengths, while A. nordenskioeldii did not fluoresce. The hypnozygotes of S. nivaloides emitted brighter fluorescence than the cysts, and the most intense luminosity was observed in the UV range. The Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDS) spectroscopic analysis showed differences in the chemical composition between samples collected from three different sites. Samples dominated by cyst cells were characterized by the presence of an abundant polysaccharide envelope. Article in Journal/Newspaper Arctic glacier Svalbard Springer Nature (via Crossref) Arctic Svalbard Scientific Reports 11 1 |
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Open Polar |
collection |
Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
Multidisciplinary |
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Multidisciplinary Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
topic_facet |
Multidisciplinary |
description |
Abstract The results show the morphological analyses and spectroscopic studies of snow and glacier algae and their parasitic fungi in Svalbard (High Arctic). Fixed algal cells of two species, Sanguina nivaloides and Ancylonema nordenskioeldii, were imaged using light microscopy, scanning electron microscopy (SEM), and atomic force microscopy (AFM). Fluorescence microscopy using Calcofluor white stain supported the observations of parasitic fungi on the algal cells. Images in brightfield microscopy showed chytrid-like fungi penetrating the cells of both algal species. Parasites were found to colonize the cells of A. nordenskioeldii and hypnozygotes of S. nivaloides , while no fungi infected the cyst stages of S. nivaloides . The autofluorescence analysis revealed the ability of S. nivaloides to glow when excited with different wavelengths, while A. nordenskioeldii did not fluoresce. The hypnozygotes of S. nivaloides emitted brighter fluorescence than the cysts, and the most intense luminosity was observed in the UV range. The Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDS) spectroscopic analysis showed differences in the chemical composition between samples collected from three different sites. Samples dominated by cyst cells were characterized by the presence of an abundant polysaccharide envelope. |
format |
Article in Journal/Newspaper |
author |
Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn |
author_facet |
Fiołka, Marta J. Takeuchi, Nozomu Sofińska-Chmiel, Weronika Wójcik-Mieszawska, Sylwia Irvine-Fynn, Tristram Edwards, Arwyn |
author_sort |
Fiołka, Marta J. |
title |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_short |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_full |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_fullStr |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_full_unstemmed |
Morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of Svalbard |
title_sort |
morphological and spectroscopic analysis of snow and glacier algae and their parasitic fungi on different glaciers of svalbard |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1038/s41598-021-01211-8 https://www.nature.com/articles/s41598-021-01211-8.pdf https://www.nature.com/articles/s41598-021-01211-8 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic glacier Svalbard |
genre_facet |
Arctic glacier Svalbard |
op_source |
Scientific Reports volume 11, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-021-01211-8 |
container_title |
Scientific Reports |
container_volume |
11 |
container_issue |
1 |
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1766338388220182528 |