Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog
Abstract Wolf ( Canis lupus ) is a species included in appendices of CITES and is often encountered in cases of alleged poaching and trafficking of their products. When such crimes are suspected, those involved may attempt to evade legal action by claiming that the animals involved are domestic dogs...
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2020
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Online Access: | http://dx.doi.org/10.1038/s41598-020-70225-5 https://www.nature.com/articles/s41598-020-70225-5.pdf https://www.nature.com/articles/s41598-020-70225-5 |
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crspringernat:10.1038/s41598-020-70225-5 2023-05-15T15:50:36+02:00 Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog Jiang, Hong Hui Li, Bo Ma, Yue Bai, Su Ying Dahmer, Thomas D. Linacre, Adrian Xu, Yan Chun Fundamental Research Funds for the Central Universities of China Wildlife Conservation and Management Project of State Forestry and Grassland Administration of China 2020 http://dx.doi.org/10.1038/s41598-020-70225-5 https://www.nature.com/articles/s41598-020-70225-5.pdf https://www.nature.com/articles/s41598-020-70225-5 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 10, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2020 crspringernat https://doi.org/10.1038/s41598-020-70225-5 2022-01-04T14:08:37Z Abstract Wolf ( Canis lupus ) is a species included in appendices of CITES and is often encountered in cases of alleged poaching and trafficking of their products. When such crimes are suspected, those involved may attempt to evade legal action by claiming that the animals involved are domestic dogs ( C. l. familiaris ). To respond effectively to such claims, law enforcement agencies require reliable and robust methods to distinguish wolves from dogs. Reported molecular genetic methods are either unreliable (mitogenome sequence based), or operationally cumbersome and require much DNA (un-multiplexed microsatellites), or financially expensive (genome wide SNP genotyping). We report on the validation of a panel of 12 ancestral informative single nucleotide polymorphism (SNP) markers for discriminating wolves from dogs. A SNaPshot multiplex genotyping system was developed for the panel, and 97 Mongolian wolves ( C. l. chanco ) and 108 domestic dogs were used for validation. Results showed this panel had high genotyping success (0.991), reproducibility (1.00) and origin assignment accuracy (0.97 ± 0.05 for dogs and 1.00 ± 0.03 for wolves). Species-specificity testing suggested strong tolerance to DNA contamination across species, except for Canidae. The minimum DNA required for reliable genotyping was 6.25 pg/μl. The method and established gene frequency database are available to support identification of wolves and dogs by law enforcement agencies. Article in Journal/Newspaper Canis lupus Springer Nature (via Crossref) Scientific Reports 10 1 |
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Springer Nature (via Crossref) |
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English |
topic |
Multidisciplinary |
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Multidisciplinary Jiang, Hong Hui Li, Bo Ma, Yue Bai, Su Ying Dahmer, Thomas D. Linacre, Adrian Xu, Yan Chun Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
topic_facet |
Multidisciplinary |
description |
Abstract Wolf ( Canis lupus ) is a species included in appendices of CITES and is often encountered in cases of alleged poaching and trafficking of their products. When such crimes are suspected, those involved may attempt to evade legal action by claiming that the animals involved are domestic dogs ( C. l. familiaris ). To respond effectively to such claims, law enforcement agencies require reliable and robust methods to distinguish wolves from dogs. Reported molecular genetic methods are either unreliable (mitogenome sequence based), or operationally cumbersome and require much DNA (un-multiplexed microsatellites), or financially expensive (genome wide SNP genotyping). We report on the validation of a panel of 12 ancestral informative single nucleotide polymorphism (SNP) markers for discriminating wolves from dogs. A SNaPshot multiplex genotyping system was developed for the panel, and 97 Mongolian wolves ( C. l. chanco ) and 108 domestic dogs were used for validation. Results showed this panel had high genotyping success (0.991), reproducibility (1.00) and origin assignment accuracy (0.97 ± 0.05 for dogs and 1.00 ± 0.03 for wolves). Species-specificity testing suggested strong tolerance to DNA contamination across species, except for Canidae. The minimum DNA required for reliable genotyping was 6.25 pg/μl. The method and established gene frequency database are available to support identification of wolves and dogs by law enforcement agencies. |
author2 |
Fundamental Research Funds for the Central Universities of China Wildlife Conservation and Management Project of State Forestry and Grassland Administration of China |
format |
Article in Journal/Newspaper |
author |
Jiang, Hong Hui Li, Bo Ma, Yue Bai, Su Ying Dahmer, Thomas D. Linacre, Adrian Xu, Yan Chun |
author_facet |
Jiang, Hong Hui Li, Bo Ma, Yue Bai, Su Ying Dahmer, Thomas D. Linacre, Adrian Xu, Yan Chun |
author_sort |
Jiang, Hong Hui |
title |
Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
title_short |
Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
title_full |
Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
title_fullStr |
Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
title_full_unstemmed |
Forensic validation of a panel of 12 SNPs for identification of Mongolian wolf and dog |
title_sort |
forensic validation of a panel of 12 snps for identification of mongolian wolf and dog |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41598-020-70225-5 https://www.nature.com/articles/s41598-020-70225-5.pdf https://www.nature.com/articles/s41598-020-70225-5 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Scientific Reports volume 10, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-70225-5 |
container_title |
Scientific Reports |
container_volume |
10 |
container_issue |
1 |
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1766385594906181632 |