Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners
Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to vari...
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Springer Science and Business Media LLC
2020
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crspringernat:10.1038/s41598-020-66601-w 2023-05-15T15:27:37+02:00 Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd 2020 http://dx.doi.org/10.1038/s41598-020-66601-w http://www.nature.com/articles/s41598-020-66601-w.pdf http://www.nature.com/articles/s41598-020-66601-w en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 10, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2020 crspringernat https://doi.org/10.1038/s41598-020-66601-w 2022-01-04T07:10:31Z Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners. Article in Journal/Newspaper atlantic cod Barents Sea Springer Nature (via Crossref) Barents Sea Scientific Reports 10 1 |
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Springer Nature (via Crossref) |
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English |
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Multidisciplinary |
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Multidisciplinary Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
topic_facet |
Multidisciplinary |
description |
Abstract The collection and presentation of accurate reproductive data from wild fish has historically been somewhat problematic, especially for serially spawning species. Therefore, the aim of the current study was to develop a novel method of assessing female spawning status that is robust to variation in oocyte dynamics between specimens. Atlantic cod (Barents Sea stock) were used to develop the new ‘ultrametric’ method, that is based on the progressive depletion of the vitellogenic oocyte pool relative to the rather constant previtellogenic oocyte (PVO) pool. Fish were subsequently partitioned into one of four categories that accurately reflected changes in their oocyte size frequency distribution characteristics and gonadosomatic index throughout spawning. The ultrametric method overcomes difficulties associated with presence of bimodal oocyte distributions, oocyte tails, lack of clear hiatus region, and presence of free ova, and can be implemented at a single sampling point. Much of the workflow is fully automated, and the technique may circumvent the need for histological analysis depending on the desired outcome. The ultrametric method differs from the traditional autodiametric method in that PVOs can be separated by ultrasonication and then enumerated, and ovarian homogeneity is not a mandatory requirement per se. The method is designed for determinate spawners but might be extended to include indeterminate spawners. |
format |
Article in Journal/Newspaper |
author |
Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd |
author_facet |
Anderson, Kelli C. Alix, Maud Charitonidou, Katerina Thorsen, Anders Thorsheim, Grethe Ganias, Kostas Schmidt, Thassya C. dos Santos Kjesbu, Olav Sigurd |
author_sort |
Anderson, Kelli C. |
title |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_short |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_full |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_fullStr |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_full_unstemmed |
Development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
title_sort |
development of a new ‘ultrametric’ method for assessing spawning progression in female teleost serial spawners |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41598-020-66601-w http://www.nature.com/articles/s41598-020-66601-w.pdf http://www.nature.com/articles/s41598-020-66601-w |
geographic |
Barents Sea |
geographic_facet |
Barents Sea |
genre |
atlantic cod Barents Sea |
genre_facet |
atlantic cod Barents Sea |
op_source |
Scientific Reports volume 10, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-66601-w |
container_title |
Scientific Reports |
container_volume |
10 |
container_issue |
1 |
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1766358044951707648 |