Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number
Abstract The master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome ( sdY ), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY -...
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2020
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crspringernat:10.1038/s41598-020-66406-x 2023-05-15T15:30:38+02:00 Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number Brown, Morgan S. Evans, Brad S. Afonso, Luis O. B. 2020 http://dx.doi.org/10.1038/s41598-020-66406-x http://www.nature.com/articles/s41598-020-66406-x.pdf http://www.nature.com/articles/s41598-020-66406-x en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 10, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2020 crspringernat https://doi.org/10.1038/s41598-020-66406-x 2022-01-04T07:17:51Z Abstract The master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome ( sdY ), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY -predicted genotypic sex and phenotypic sex in these species is unclear. Using an established multiplex PCR test for exons 2 and 3 of sdY , we demonstrated that sdY -predicted genotypic sex was discordant with histologically evidenced phenotypic sex in 4% of 176 Tasmanian Atlantic salmon. All discordant individuals were phenotypic females presenting a male genotype. Using real-time qPCR assays that we developed and validated for exons 2, 3 and 4 of sdY , all genotype-phenotype discordant females were confirmed to possess sdY , albeit at a reduced number of copies when compared to phenotypic males. The real-time qPCR assays also demonstrated reduced levels of sdY in 30% of phenotypic females that the established multiplex PCR-based test indicated to be devoid of sdY . These findings suggest sdY may be reduced in copy number or mosaicked in the genomic DNA of sdY -positive phenotypic female Atlantic salmon and highlight the importance of understanding the effects of reduced sdY copies on the development of phenotypic sex. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) The ''Y'' ENVELOPE(-112.453,-112.453,57.591,57.591) Scientific Reports 10 1 |
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Springer Nature (via Crossref) |
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English |
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Multidisciplinary |
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Multidisciplinary Brown, Morgan S. Evans, Brad S. Afonso, Luis O. B. Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
topic_facet |
Multidisciplinary |
description |
Abstract The master sex determinant in rainbow trout (Oncorhynchus mykiss), sexually dimorphic on the Y chromosome ( sdY ), is strongly but not perfectly associated with male phenotype in several other species from the family Salmonidae. Currently, the cause and implications of discordance for sdY -predicted genotypic sex and phenotypic sex in these species is unclear. Using an established multiplex PCR test for exons 2 and 3 of sdY , we demonstrated that sdY -predicted genotypic sex was discordant with histologically evidenced phenotypic sex in 4% of 176 Tasmanian Atlantic salmon. All discordant individuals were phenotypic females presenting a male genotype. Using real-time qPCR assays that we developed and validated for exons 2, 3 and 4 of sdY , all genotype-phenotype discordant females were confirmed to possess sdY , albeit at a reduced number of copies when compared to phenotypic males. The real-time qPCR assays also demonstrated reduced levels of sdY in 30% of phenotypic females that the established multiplex PCR-based test indicated to be devoid of sdY . These findings suggest sdY may be reduced in copy number or mosaicked in the genomic DNA of sdY -positive phenotypic female Atlantic salmon and highlight the importance of understanding the effects of reduced sdY copies on the development of phenotypic sex. |
format |
Article in Journal/Newspaper |
author |
Brown, Morgan S. Evans, Brad S. Afonso, Luis O. B. |
author_facet |
Brown, Morgan S. Evans, Brad S. Afonso, Luis O. B. |
author_sort |
Brown, Morgan S. |
title |
Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
title_short |
Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
title_full |
Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
title_fullStr |
Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
title_full_unstemmed |
Discordance for genotypic sex in phenotypic female Atlantic salmon (Salmo salar) is related to a reduced sdY copy number |
title_sort |
discordance for genotypic sex in phenotypic female atlantic salmon (salmo salar) is related to a reduced sdy copy number |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41598-020-66406-x http://www.nature.com/articles/s41598-020-66406-x.pdf http://www.nature.com/articles/s41598-020-66406-x |
long_lat |
ENVELOPE(-112.453,-112.453,57.591,57.591) |
geographic |
The ''Y'' |
geographic_facet |
The ''Y'' |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Scientific Reports volume 10, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-66406-x |
container_title |
Scientific Reports |
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10 |
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1 |
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1766361076853637120 |