Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean
Abstract Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homol...
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2020
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Online Access: | http://dx.doi.org/10.1038/s41598-020-58023-5 http://www.nature.com/articles/s41598-020-58023-5.pdf http://www.nature.com/articles/s41598-020-58023-5 |
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crspringernat:10.1038/s41598-020-58023-5 2023-05-15T14:06:57+02:00 Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean Kim, Se-Hwan Jung, ByungHoon Hong, Soon Gyu Jung, Kwang-Hwan 2020 http://dx.doi.org/10.1038/s41598-020-58023-5 http://www.nature.com/articles/s41598-020-58023-5.pdf http://www.nature.com/articles/s41598-020-58023-5 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 10, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2020 crspringernat https://doi.org/10.1038/s41598-020-58023-5 2022-01-04T10:35:11Z Abstract Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by PCR from the seawater sample near King George Island, Antarctica. One was the same sequence as the first reported GPR (Green-light absorbing PR) from Monterey Bay. Another named HSG119 was a newly discovered sequence which shows high sequence similarity with BPR (Blue-light absorbing PR). HSG119 has an absorption maximum at 493 nm with broader spectrum at pH7.0 and it can pump protons out of the cell membrane. Interestingly, it showed a similar temperature dependence to GPR(Y200N) that isolated near the North pole. Article in Journal/Newspaper Antarc* Antarctica antartic* King George Island North Pole Springer Nature (via Crossref) King George Island North Pole Scientific Reports 10 1 |
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Springer Nature (via Crossref) |
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crspringernat |
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English |
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Multidisciplinary |
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Multidisciplinary Kim, Se-Hwan Jung, ByungHoon Hong, Soon Gyu Jung, Kwang-Hwan Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
topic_facet |
Multidisciplinary |
description |
Abstract Proteorhodopsin (PR) is discovered from marine bacteria and it has proton pumping activity from inside to outside of the cell using light energy. In general, PR classified into two groups by the maximum absorption spectra. In this study, we isolated the two of a full sequence of opsin homologues by PCR from the seawater sample near King George Island, Antarctica. One was the same sequence as the first reported GPR (Green-light absorbing PR) from Monterey Bay. Another named HSG119 was a newly discovered sequence which shows high sequence similarity with BPR (Blue-light absorbing PR). HSG119 has an absorption maximum at 493 nm with broader spectrum at pH7.0 and it can pump protons out of the cell membrane. Interestingly, it showed a similar temperature dependence to GPR(Y200N) that isolated near the North pole. |
format |
Article in Journal/Newspaper |
author |
Kim, Se-Hwan Jung, ByungHoon Hong, Soon Gyu Jung, Kwang-Hwan |
author_facet |
Kim, Se-Hwan Jung, ByungHoon Hong, Soon Gyu Jung, Kwang-Hwan |
author_sort |
Kim, Se-Hwan |
title |
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
title_short |
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
title_full |
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
title_fullStr |
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
title_full_unstemmed |
Temperature Dependency of Proton Pumping Activity for Marine Microbial Rhodopsin from Antartic Ocean |
title_sort |
temperature dependency of proton pumping activity for marine microbial rhodopsin from antartic ocean |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41598-020-58023-5 http://www.nature.com/articles/s41598-020-58023-5.pdf http://www.nature.com/articles/s41598-020-58023-5 |
geographic |
King George Island North Pole |
geographic_facet |
King George Island North Pole |
genre |
Antarc* Antarctica antartic* King George Island North Pole |
genre_facet |
Antarc* Antarctica antartic* King George Island North Pole |
op_source |
Scientific Reports volume 10, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-020-58023-5 |
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Scientific Reports |
container_volume |
10 |
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1 |
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1766278950383779840 |