Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea
Abstract Several mud volcanoes are active in the Canadian Beaufort Sea. In this study, we investigated vertical variations in methanotrophic communities in sediments of the mud volcano MV420 (420 m water depth) by analyzing geochemical properties, microbial lipids, and nucleic acid signatures. Three...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2019
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1038/s41598-019-53950-4 http://www.nature.com/articles/s41598-019-53950-4.pdf http://www.nature.com/articles/s41598-019-53950-4 |
id |
crspringernat:10.1038/s41598-019-53950-4 |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1038/s41598-019-53950-4 2023-05-15T15:40:20+02:00 Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea Lee, Dong-Hun Lee, Yung Mi Kim, Jung-Hyun Jin, Young Keun Paull, Charles Niemann, Helge Kim, Ji-Hoon Shin, Kyung-Hoon Korea Institute of Marine Science and Technology Promotion 2019 http://dx.doi.org/10.1038/s41598-019-53950-4 http://www.nature.com/articles/s41598-019-53950-4.pdf http://www.nature.com/articles/s41598-019-53950-4 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 9, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2019 crspringernat https://doi.org/10.1038/s41598-019-53950-4 2022-01-04T15:16:01Z Abstract Several mud volcanoes are active in the Canadian Beaufort Sea. In this study, we investigated vertical variations in methanotrophic communities in sediments of the mud volcano MV420 (420 m water depth) by analyzing geochemical properties, microbial lipids, and nucleic acid signatures. Three push cores were collected with a remotely operated vehicle from visually discriminative habitats that were devoid of megafauna and/microbial mats (DM) to the naked eye, covered with bacterial mats (BM), or colonized by siboglinid tubeworms (ST). All MV420 sites showed the presence of aerobic methane oxidation (MOx)- and anaerobic methane oxidation (AOM)-related lipid biomarkers (4α-methyl sterols and sn -2-hydroxyarchaeol, respectively), which were distinctly different in comparison with a reference site at which these compounds were not detected. Lipid biomarker results were in close agreement with 16S rRNA analyses, which revealed the presence of MOx-related bacteria ( Methylococcales ) and AOM-related archaea (ANME-2 and ANME-3) at the MV420 sites. 4α-methyl sterols derived from Methylococcales predominated in the surface layer at the BM site, which showed a moderate methane flux (0.04 mmol cm −2 y −1 ), while their occurrence was limited at the DM (0.06 mmol cm −2 y −1 ) and ST (0.01 mmol cm −2 y −1 ) sites. On the other hand, 13 C-depleted sn -2-hydroxyarchaeol potentially derived from ANME-2 and/or ANME-3 was abundant in down-core sediments at the ST site. Our study indicates that a niche diversification within this mud volcano system has shaped distinct methanotrophic communities due to availability of electron acceptors in association with varying degrees of methane flux and bioirrigation activity. Article in Journal/Newspaper Beaufort Sea Springer Nature (via Crossref) Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Multidisciplinary |
spellingShingle |
Multidisciplinary Lee, Dong-Hun Lee, Yung Mi Kim, Jung-Hyun Jin, Young Keun Paull, Charles Niemann, Helge Kim, Ji-Hoon Shin, Kyung-Hoon Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
topic_facet |
Multidisciplinary |
description |
Abstract Several mud volcanoes are active in the Canadian Beaufort Sea. In this study, we investigated vertical variations in methanotrophic communities in sediments of the mud volcano MV420 (420 m water depth) by analyzing geochemical properties, microbial lipids, and nucleic acid signatures. Three push cores were collected with a remotely operated vehicle from visually discriminative habitats that were devoid of megafauna and/microbial mats (DM) to the naked eye, covered with bacterial mats (BM), or colonized by siboglinid tubeworms (ST). All MV420 sites showed the presence of aerobic methane oxidation (MOx)- and anaerobic methane oxidation (AOM)-related lipid biomarkers (4α-methyl sterols and sn -2-hydroxyarchaeol, respectively), which were distinctly different in comparison with a reference site at which these compounds were not detected. Lipid biomarker results were in close agreement with 16S rRNA analyses, which revealed the presence of MOx-related bacteria ( Methylococcales ) and AOM-related archaea (ANME-2 and ANME-3) at the MV420 sites. 4α-methyl sterols derived from Methylococcales predominated in the surface layer at the BM site, which showed a moderate methane flux (0.04 mmol cm −2 y −1 ), while their occurrence was limited at the DM (0.06 mmol cm −2 y −1 ) and ST (0.01 mmol cm −2 y −1 ) sites. On the other hand, 13 C-depleted sn -2-hydroxyarchaeol potentially derived from ANME-2 and/or ANME-3 was abundant in down-core sediments at the ST site. Our study indicates that a niche diversification within this mud volcano system has shaped distinct methanotrophic communities due to availability of electron acceptors in association with varying degrees of methane flux and bioirrigation activity. |
author2 |
Korea Institute of Marine Science and Technology Promotion |
format |
Article in Journal/Newspaper |
author |
Lee, Dong-Hun Lee, Yung Mi Kim, Jung-Hyun Jin, Young Keun Paull, Charles Niemann, Helge Kim, Ji-Hoon Shin, Kyung-Hoon |
author_facet |
Lee, Dong-Hun Lee, Yung Mi Kim, Jung-Hyun Jin, Young Keun Paull, Charles Niemann, Helge Kim, Ji-Hoon Shin, Kyung-Hoon |
author_sort |
Lee, Dong-Hun |
title |
Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
title_short |
Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
title_full |
Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
title_fullStr |
Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
title_full_unstemmed |
Discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the Canadian Beaufort Sea |
title_sort |
discriminative biogeochemical signatures of methanotrophs in different chemosynthetic habitats at an active mud volcano in the canadian beaufort sea |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1038/s41598-019-53950-4 http://www.nature.com/articles/s41598-019-53950-4.pdf http://www.nature.com/articles/s41598-019-53950-4 |
genre |
Beaufort Sea |
genre_facet |
Beaufort Sea |
op_source |
Scientific Reports volume 9, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-53950-4 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766372550739230720 |