Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms
Abstract Most fish-allergic patients have anti-β-parvalbumin (β-PV) immunoglobulin E (IgE), which cross-reacts among fish species with variable clinical effects. Although the β-PV load is considered a determinant for allergenicity, fish species express distinct β-PV isoforms with unknown pathogenic...
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Online Access: | http://dx.doi.org/10.1038/s41598-019-52801-6 http://www.nature.com/articles/s41598-019-52801-6.pdf http://www.nature.com/articles/s41598-019-52801-6 |
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crspringernat:10.1038/s41598-019-52801-6 2023-05-15T16:18:58+02:00 Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms Pérez-Tavarez, Raquel Carrera, Mónica Pedrosa, María Quirce, Santiago Rodríguez-Pérez, Rosa Gasset, María Ministry of Economy and Competitiveness | Agencia Estatal de Investigación Ramón Areces Foundation Angulas Aguinaga 2019 http://dx.doi.org/10.1038/s41598-019-52801-6 http://www.nature.com/articles/s41598-019-52801-6.pdf http://www.nature.com/articles/s41598-019-52801-6 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 9, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2019 crspringernat https://doi.org/10.1038/s41598-019-52801-6 2022-01-04T09:44:10Z Abstract Most fish-allergic patients have anti-β-parvalbumin (β-PV) immunoglobulin E (IgE), which cross-reacts among fish species with variable clinical effects. Although the β-PV load is considered a determinant for allergenicity, fish species express distinct β-PV isoforms with unknown pathogenic contributions. To identify the role various parameters play in allergenicity, we have taken Gadus morhua and Scomber japonicus models, determined their β-PV isoform composition and analyzed the interaction of the IgE from fish-allergic patient sera with these different conformations. We found that each fish species contains a major and a minor isoform, with the total PV content four times higher in Gadus morhua than in Scomber japonicus . The isoforms showing the best IgE recognition displayed protease-sensitive globular folds, and if forming amyloids, they were not immunoreactive. Of the isoforms displaying stable globular folds, one was not recognized by IgE under any of the conditions, and the other formed highly immunoreactive amyloids. The results showed that Gadus morhua muscles are equipped with an isoform combination and content that ensures the IgE recognition of all PV folds, whereas the allergenic load of Scomber japonicus is under the control of proteolysis. We conclude that the consideration of isoform properties and content may improve the explanation of fish species allergenicity differences. Article in Journal/Newspaper Gadus morhua Springer Nature (via Crossref) Scientific Reports 9 1 |
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Springer Nature (via Crossref) |
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English |
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Multidisciplinary |
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Multidisciplinary Pérez-Tavarez, Raquel Carrera, Mónica Pedrosa, María Quirce, Santiago Rodríguez-Pérez, Rosa Gasset, María Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
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Multidisciplinary |
description |
Abstract Most fish-allergic patients have anti-β-parvalbumin (β-PV) immunoglobulin E (IgE), which cross-reacts among fish species with variable clinical effects. Although the β-PV load is considered a determinant for allergenicity, fish species express distinct β-PV isoforms with unknown pathogenic contributions. To identify the role various parameters play in allergenicity, we have taken Gadus morhua and Scomber japonicus models, determined their β-PV isoform composition and analyzed the interaction of the IgE from fish-allergic patient sera with these different conformations. We found that each fish species contains a major and a minor isoform, with the total PV content four times higher in Gadus morhua than in Scomber japonicus . The isoforms showing the best IgE recognition displayed protease-sensitive globular folds, and if forming amyloids, they were not immunoreactive. Of the isoforms displaying stable globular folds, one was not recognized by IgE under any of the conditions, and the other formed highly immunoreactive amyloids. The results showed that Gadus morhua muscles are equipped with an isoform combination and content that ensures the IgE recognition of all PV folds, whereas the allergenic load of Scomber japonicus is under the control of proteolysis. We conclude that the consideration of isoform properties and content may improve the explanation of fish species allergenicity differences. |
author2 |
Ministry of Economy and Competitiveness | Agencia Estatal de Investigación Ramón Areces Foundation Angulas Aguinaga |
format |
Article in Journal/Newspaper |
author |
Pérez-Tavarez, Raquel Carrera, Mónica Pedrosa, María Quirce, Santiago Rodríguez-Pérez, Rosa Gasset, María |
author_facet |
Pérez-Tavarez, Raquel Carrera, Mónica Pedrosa, María Quirce, Santiago Rodríguez-Pérez, Rosa Gasset, María |
author_sort |
Pérez-Tavarez, Raquel |
title |
Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
title_short |
Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
title_full |
Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
title_fullStr |
Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
title_full_unstemmed |
Reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
title_sort |
reconstruction of fish allergenicity from the content and structural traits of the component β-parvalbumin isoforms |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1038/s41598-019-52801-6 http://www.nature.com/articles/s41598-019-52801-6.pdf http://www.nature.com/articles/s41598-019-52801-6 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Scientific Reports volume 9, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-52801-6 |
container_title |
Scientific Reports |
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9 |
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1 |
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1766005251667656704 |