Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba
Abstract Antarctic krill ( Euphausia superba ) is a high latitude pelagic organism which plays a central role in the Southern Ocean ecosystem. E . superba shows daily and seasonal rhythms in physiology and behaviour, which are synchronized with the environmental cycles of its habitat. Recently, the...
Published in: | Scientific Reports |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2019
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1038/s41598-019-50282-1 http://www.nature.com/articles/s41598-019-50282-1.pdf http://www.nature.com/articles/s41598-019-50282-1 |
id |
crspringernat:10.1038/s41598-019-50282-1 |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1038/s41598-019-50282-1 2023-05-15T14:12:07+02:00 Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba Biscontin, Alberto Martini, Paolo Costa, Rodolfo Kramer, Achim Meyer, Bettina Kawaguchi, So Teschke, Mathias De Pittà, Cristiano 2019 http://dx.doi.org/10.1038/s41598-019-50282-1 http://www.nature.com/articles/s41598-019-50282-1.pdf http://www.nature.com/articles/s41598-019-50282-1 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Scientific Reports volume 9, issue 1 ISSN 2045-2322 Multidisciplinary journal-article 2019 crspringernat https://doi.org/10.1038/s41598-019-50282-1 2022-01-14T15:40:09Z Abstract Antarctic krill ( Euphausia superba ) is a high latitude pelagic organism which plays a central role in the Southern Ocean ecosystem. E . superba shows daily and seasonal rhythms in physiology and behaviour, which are synchronized with the environmental cycles of its habitat. Recently, the main components of the krill circadian machinery have been identified and characterized. However, the exact mechanisms through which the endogenous timing system operates the control and regulation of the overt rhythms remains only partially understood. Here we investigate the involvement of the circadian clock in the temporal orchestration of gene expression by using a newly developed version of a krill microarray platform. The analysis of transcriptome data from krill exposed to both light-dark cycles (LD 18:6) and constant darkness (DD), has led to the identification of 1,564 putative clock-controlled genes. A remarkably large proportion of such genes, including several clock components ( clock , period , cry2 , vrille , and slimb ), show oscillatory expression patterns in DD, with a periodicity shorter than 24 hours. Energy-storage pathways appear to be regulated by the endogenous clock in accordance with their ecological relevance in daily energy managing and overwintering. Our results provide the first representation of the krill circadian transcriptome under laboratory, free-running conditions. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean Springer Nature (via Crossref) Antarctic Southern Ocean The Antarctic Scientific Reports 9 1 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Multidisciplinary |
spellingShingle |
Multidisciplinary Biscontin, Alberto Martini, Paolo Costa, Rodolfo Kramer, Achim Meyer, Bettina Kawaguchi, So Teschke, Mathias De Pittà, Cristiano Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
topic_facet |
Multidisciplinary |
description |
Abstract Antarctic krill ( Euphausia superba ) is a high latitude pelagic organism which plays a central role in the Southern Ocean ecosystem. E . superba shows daily and seasonal rhythms in physiology and behaviour, which are synchronized with the environmental cycles of its habitat. Recently, the main components of the krill circadian machinery have been identified and characterized. However, the exact mechanisms through which the endogenous timing system operates the control and regulation of the overt rhythms remains only partially understood. Here we investigate the involvement of the circadian clock in the temporal orchestration of gene expression by using a newly developed version of a krill microarray platform. The analysis of transcriptome data from krill exposed to both light-dark cycles (LD 18:6) and constant darkness (DD), has led to the identification of 1,564 putative clock-controlled genes. A remarkably large proportion of such genes, including several clock components ( clock , period , cry2 , vrille , and slimb ), show oscillatory expression patterns in DD, with a periodicity shorter than 24 hours. Energy-storage pathways appear to be regulated by the endogenous clock in accordance with their ecological relevance in daily energy managing and overwintering. Our results provide the first representation of the krill circadian transcriptome under laboratory, free-running conditions. |
format |
Article in Journal/Newspaper |
author |
Biscontin, Alberto Martini, Paolo Costa, Rodolfo Kramer, Achim Meyer, Bettina Kawaguchi, So Teschke, Mathias De Pittà, Cristiano |
author_facet |
Biscontin, Alberto Martini, Paolo Costa, Rodolfo Kramer, Achim Meyer, Bettina Kawaguchi, So Teschke, Mathias De Pittà, Cristiano |
author_sort |
Biscontin, Alberto |
title |
Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
title_short |
Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
title_full |
Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
title_fullStr |
Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
title_full_unstemmed |
Analysis of the circadian transcriptome of the Antarctic krill Euphausia superba |
title_sort |
analysis of the circadian transcriptome of the antarctic krill euphausia superba |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1038/s41598-019-50282-1 http://www.nature.com/articles/s41598-019-50282-1.pdf http://www.nature.com/articles/s41598-019-50282-1 |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
genre_facet |
Antarc* Antarctic Antarctic Krill Euphausia superba Southern Ocean |
op_source |
Scientific Reports volume 9, issue 1 ISSN 2045-2322 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41598-019-50282-1 |
container_title |
Scientific Reports |
container_volume |
9 |
container_issue |
1 |
_version_ |
1766284384269238272 |