Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment
Abstract Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade th...
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Online Access: | http://dx.doi.org/10.1038/s41396-020-00817-6 http://www.nature.com/articles/s41396-020-00817-6.pdf http://www.nature.com/articles/s41396-020-00817-6 |
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crspringernat:10.1038/s41396-020-00817-6 2023-05-15T18:28:14+02:00 Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander Austrian Science Fund 2020 http://dx.doi.org/10.1038/s41396-020-00817-6 http://www.nature.com/articles/s41396-020-00817-6.pdf http://www.nature.com/articles/s41396-020-00817-6 en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY The ISME Journal volume 15, issue 3, page 833-847 ISSN 1751-7362 1751-7370 Ecology, Evolution, Behavior and Systematics Microbiology journal-article 2020 crspringernat https://doi.org/10.1038/s41396-020-00817-6 2022-01-14T15:44:10Z Abstract Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain poorly resolved. Here, we revealed identities, trophic interactions, and genomic features of bacteria that degraded 13 C-labeled proteins and lipids in cold anoxic microcosms containing sulfidic subarctic marine sediment. Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within 5 days. DNA-stable isotope probing (SIP) suggested Psychrilyobacter atlanticus was an important primary degrader of proteins, and Psychromonas members were important primary degraders of both proteins and lipids. Closely related Psychromonas populations, as represented by distinct 16S rRNA gene variants, differentially utilized either proteins or lipids. DNA-SIP also showed 13 C-labeling of various Deltaproteobacteria within 10 days, indicating trophic transfer of carbon to putative sulfate-reducers. Metagenome-assembled genomes revealed the primary hydrolyzers encoded secreted peptidases or lipases, and enzymes for catabolism of protein or lipid degradation products. Psychromonas species are prevalent in diverse marine sediments, suggesting they are important players in organic carbon processing in situ. Together, this study provides new insights into the identities, functions, and genomes of bacteria that actively degrade abundant necromass macromolecules in the seafloor. Article in Journal/Newspaper Subarctic Springer Nature (via Crossref) The ISME Journal 15 3 833 847 |
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Ecology, Evolution, Behavior and Systematics Microbiology |
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Ecology, Evolution, Behavior and Systematics Microbiology Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
topic_facet |
Ecology, Evolution, Behavior and Systematics Microbiology |
description |
Abstract Microorganisms in marine sediments play major roles in marine biogeochemical cycles by mineralizing substantial quantities of organic matter from decaying cells. Proteins and lipids are abundant components of necromass, yet the taxonomic identities of microorganisms that actively degrade them remain poorly resolved. Here, we revealed identities, trophic interactions, and genomic features of bacteria that degraded 13 C-labeled proteins and lipids in cold anoxic microcosms containing sulfidic subarctic marine sediment. Supplemented proteins and lipids were rapidly fermented to various volatile fatty acids within 5 days. DNA-stable isotope probing (SIP) suggested Psychrilyobacter atlanticus was an important primary degrader of proteins, and Psychromonas members were important primary degraders of both proteins and lipids. Closely related Psychromonas populations, as represented by distinct 16S rRNA gene variants, differentially utilized either proteins or lipids. DNA-SIP also showed 13 C-labeling of various Deltaproteobacteria within 10 days, indicating trophic transfer of carbon to putative sulfate-reducers. Metagenome-assembled genomes revealed the primary hydrolyzers encoded secreted peptidases or lipases, and enzymes for catabolism of protein or lipid degradation products. Psychromonas species are prevalent in diverse marine sediments, suggesting they are important players in organic carbon processing in situ. Together, this study provides new insights into the identities, functions, and genomes of bacteria that actively degrade abundant necromass macromolecules in the seafloor. |
author2 |
Austrian Science Fund |
format |
Article in Journal/Newspaper |
author |
Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander |
author_facet |
Pelikan, Claus Wasmund, Kenneth Glombitza, Clemens Hausmann, Bela Herbold, Craig W. Flieder, Mathias Loy, Alexander |
author_sort |
Pelikan, Claus |
title |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_short |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_full |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_fullStr |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_full_unstemmed |
Anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
title_sort |
anaerobic bacterial degradation of protein and lipid macromolecules in subarctic marine sediment |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1038/s41396-020-00817-6 http://www.nature.com/articles/s41396-020-00817-6.pdf http://www.nature.com/articles/s41396-020-00817-6 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
The ISME Journal volume 15, issue 3, page 833-847 ISSN 1751-7362 1751-7370 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1038/s41396-020-00817-6 |
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The ISME Journal |
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15 |
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3 |
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833 |
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847 |
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1766210628589977600 |