Clinal variation in growth cessation and FTL2 expression in Siberian spruce
Abstract Forest trees exhibit strong patterns of local adaptation in phenological traits along latitudinal gradients. Previous studies in spruce have shown that variation at genes from the photoperiodic pathway and the circadian clock are associated to these clines but it has been difficult to find...
Published in: | Tree Genetics & Genomes |
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Springer Science and Business Media LLC
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Online Access: | http://dx.doi.org/10.1007/s11295-019-1389-7 http://link.springer.com/content/pdf/10.1007/s11295-019-1389-7.pdf http://link.springer.com/article/10.1007/s11295-019-1389-7/fulltext.html |
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crspringernat:10.1007/s11295-019-1389-7 2023-05-15T17:48:49+02:00 Clinal variation in growth cessation and FTL2 expression in Siberian spruce Li, Lili Chen, Jun Lascoux, Martin Svenska Forskningsrådet Formas 2019 http://dx.doi.org/10.1007/s11295-019-1389-7 http://link.springer.com/content/pdf/10.1007/s11295-019-1389-7.pdf http://link.springer.com/article/10.1007/s11295-019-1389-7/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Tree Genetics & Genomes volume 15, issue 6 ISSN 1614-2942 1614-2950 Horticulture Genetics Molecular Biology Forestry journal-article 2019 crspringernat https://doi.org/10.1007/s11295-019-1389-7 2022-01-04T15:44:59Z Abstract Forest trees exhibit strong patterns of local adaptation in phenological traits along latitudinal gradients. Previous studies in spruce have shown that variation at genes from the photoperiodic pathway and the circadian clock are associated to these clines but it has been difficult to find solid evidence of selection for some of these genes. Here, we used growth cessation, gene expression, and single nucleotide polymorphism (SNP) data at two major candidate loci, FLOWERING LOCUS T/TERMINAL FLOWER1-Like2 ( FTL2 ) and GIGANTEA ( GI ), as well as at background loci from a latitudinal gradient in Siberian spruce ( Picea obovata ) populations along the Ob River to test for clinal variation in growth cessation and at the two candidate genes. As in previous studies, there was a strong latitudinal cline in growth cessation that was accompanied by a significant cline in the expression of FTL2 . Expression of FTL2 was significantly associated with allele frequencies at some of the GI ’s SNPs. However, the cline in allele frequency at candidate genes was not as steep as in a Norway spruce cline and in a parallel Siberian spruce cline studied previously and nonsignificant when a correction for population structure was applied. A McDonald-Kreitman test did not detect decisive evidence of selection on GI ( p value = 0.07) and could not be applied to FTL2 because of limited polymorphism. Nonetheless, polymorphisms contributed more to the increased neutrality index of Po GI than to that of control loci. Finally, comparing the results of two previously published studies to our new dataset led to the identification of strong candidate SNPs for local adaptation in FTL2 promoter and GI . Article in Journal/Newspaper ob river Springer Nature (via Crossref) Norway Tree Genetics & Genomes 15 6 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Horticulture Genetics Molecular Biology Forestry |
spellingShingle |
Horticulture Genetics Molecular Biology Forestry Li, Lili Chen, Jun Lascoux, Martin Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
topic_facet |
Horticulture Genetics Molecular Biology Forestry |
description |
Abstract Forest trees exhibit strong patterns of local adaptation in phenological traits along latitudinal gradients. Previous studies in spruce have shown that variation at genes from the photoperiodic pathway and the circadian clock are associated to these clines but it has been difficult to find solid evidence of selection for some of these genes. Here, we used growth cessation, gene expression, and single nucleotide polymorphism (SNP) data at two major candidate loci, FLOWERING LOCUS T/TERMINAL FLOWER1-Like2 ( FTL2 ) and GIGANTEA ( GI ), as well as at background loci from a latitudinal gradient in Siberian spruce ( Picea obovata ) populations along the Ob River to test for clinal variation in growth cessation and at the two candidate genes. As in previous studies, there was a strong latitudinal cline in growth cessation that was accompanied by a significant cline in the expression of FTL2 . Expression of FTL2 was significantly associated with allele frequencies at some of the GI ’s SNPs. However, the cline in allele frequency at candidate genes was not as steep as in a Norway spruce cline and in a parallel Siberian spruce cline studied previously and nonsignificant when a correction for population structure was applied. A McDonald-Kreitman test did not detect decisive evidence of selection on GI ( p value = 0.07) and could not be applied to FTL2 because of limited polymorphism. Nonetheless, polymorphisms contributed more to the increased neutrality index of Po GI than to that of control loci. Finally, comparing the results of two previously published studies to our new dataset led to the identification of strong candidate SNPs for local adaptation in FTL2 promoter and GI . |
author2 |
Svenska Forskningsrådet Formas |
format |
Article in Journal/Newspaper |
author |
Li, Lili Chen, Jun Lascoux, Martin |
author_facet |
Li, Lili Chen, Jun Lascoux, Martin |
author_sort |
Li, Lili |
title |
Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
title_short |
Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
title_full |
Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
title_fullStr |
Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
title_full_unstemmed |
Clinal variation in growth cessation and FTL2 expression in Siberian spruce |
title_sort |
clinal variation in growth cessation and ftl2 expression in siberian spruce |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1007/s11295-019-1389-7 http://link.springer.com/content/pdf/10.1007/s11295-019-1389-7.pdf http://link.springer.com/article/10.1007/s11295-019-1389-7/fulltext.html |
geographic |
Norway |
geographic_facet |
Norway |
genre |
ob river |
genre_facet |
ob river |
op_source |
Tree Genetics & Genomes volume 15, issue 6 ISSN 1614-2942 1614-2950 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s11295-019-1389-7 |
container_title |
Tree Genetics & Genomes |
container_volume |
15 |
container_issue |
6 |
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1766154965030535168 |