Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms
Abstract The simultaneous quantification of several transcripts via multiplex PCR can accelerate research in fish physiological responses to diet and enable the development of superior aquafeeds for farmed fish. We designed two multiplex PCR panels that included assays for 40 biomarker genes represe...
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2020
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crspringernat:10.1007/s10126-020-09972-5 2023-05-15T15:31:07+02:00 Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms Caballero-Solares, Albert Xue, Xi Cleveland, Beth M. Foroutani, Maryam Beheshti Parrish, Christopher C. Taylor, Richard G. Rise, Matthew L. Genome Canada Genome Atlantic Research and Development Corporation of Newfoundland and Labrador Atlantic Canada Opportunities Agency Natural Sciences and Engineering Research Council of Canada 2020 http://dx.doi.org/10.1007/s10126-020-09972-5 https://link.springer.com/content/pdf/10.1007/s10126-020-09972-5.pdf https://link.springer.com/article/10.1007/s10126-020-09972-5/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Marine Biotechnology volume 22, issue 4, page 511-525 ISSN 1436-2228 1436-2236 Applied Microbiology and Biotechnology journal-article 2020 crspringernat https://doi.org/10.1007/s10126-020-09972-5 2022-01-04T09:23:39Z Abstract The simultaneous quantification of several transcripts via multiplex PCR can accelerate research in fish physiological responses to diet and enable the development of superior aquafeeds for farmed fish. We designed two multiplex PCR panels that included assays for 40 biomarker genes representing key aspects of fish physiology (growth, metabolism, oxidative stress, and inflammation) and 3 normalizer genes. We used both panels to assess the physiological effects of replacing fish meal and fish oil by terrestrial alternatives on Atlantic salmon smolts. In a 14-week trial, we tested three diets based on marine ingredients (MAR), animal by-products and vegetable oil (ABP), and plant protein and vegetable oil (VEG). Dietary treatments affected the expression of genes involved in hepatic glucose and lipid metabolism (e.g., srebp1, elovl2 ), cell redox status (e.g., txna , prdx1b ), and inflammation (e.g., pgds , 5loxa ). At the multivariate level, gene expression profiles were more divergent between fish fed the marine and terrestrial diets (MAR vs. ABP/VEG) than between the two terrestrial diets (ABP vs. VEG). Liver ARA was inversely related to glucose metabolism ( gck )- and growth ( igfbp-5b1 , htra1b )-related biomarkers and hepatosomatic index. Liver DHA and EPA levels correlated negatively with elovl2 , whereas ARA levels correlated positively with fadsd5 . Lower hepatic EPA/ARA in ABP-fed fish correlated with the increased expression of biomarkers related to mitochondrial function ( fabp3a ), oxidative stress ( txna , prdx1b ), and inflammation ( pgds , 5loxa ). The analysis of hepatic biomarker gene expression via multiplex PCR revealed potential physiological impacts and nutrient-gene interactions in Atlantic salmon fed lower levels of marine-sourced nutrients. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) Marine Biotechnology 22 4 511 525 |
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Springer Nature (via Crossref) |
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English |
topic |
Applied Microbiology and Biotechnology |
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Applied Microbiology and Biotechnology Caballero-Solares, Albert Xue, Xi Cleveland, Beth M. Foroutani, Maryam Beheshti Parrish, Christopher C. Taylor, Richard G. Rise, Matthew L. Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
topic_facet |
Applied Microbiology and Biotechnology |
description |
Abstract The simultaneous quantification of several transcripts via multiplex PCR can accelerate research in fish physiological responses to diet and enable the development of superior aquafeeds for farmed fish. We designed two multiplex PCR panels that included assays for 40 biomarker genes representing key aspects of fish physiology (growth, metabolism, oxidative stress, and inflammation) and 3 normalizer genes. We used both panels to assess the physiological effects of replacing fish meal and fish oil by terrestrial alternatives on Atlantic salmon smolts. In a 14-week trial, we tested three diets based on marine ingredients (MAR), animal by-products and vegetable oil (ABP), and plant protein and vegetable oil (VEG). Dietary treatments affected the expression of genes involved in hepatic glucose and lipid metabolism (e.g., srebp1, elovl2 ), cell redox status (e.g., txna , prdx1b ), and inflammation (e.g., pgds , 5loxa ). At the multivariate level, gene expression profiles were more divergent between fish fed the marine and terrestrial diets (MAR vs. ABP/VEG) than between the two terrestrial diets (ABP vs. VEG). Liver ARA was inversely related to glucose metabolism ( gck )- and growth ( igfbp-5b1 , htra1b )-related biomarkers and hepatosomatic index. Liver DHA and EPA levels correlated negatively with elovl2 , whereas ARA levels correlated positively with fadsd5 . Lower hepatic EPA/ARA in ABP-fed fish correlated with the increased expression of biomarkers related to mitochondrial function ( fabp3a ), oxidative stress ( txna , prdx1b ), and inflammation ( pgds , 5loxa ). The analysis of hepatic biomarker gene expression via multiplex PCR revealed potential physiological impacts and nutrient-gene interactions in Atlantic salmon fed lower levels of marine-sourced nutrients. |
author2 |
Genome Canada Genome Atlantic Research and Development Corporation of Newfoundland and Labrador Atlantic Canada Opportunities Agency Natural Sciences and Engineering Research Council of Canada |
format |
Article in Journal/Newspaper |
author |
Caballero-Solares, Albert Xue, Xi Cleveland, Beth M. Foroutani, Maryam Beheshti Parrish, Christopher C. Taylor, Richard G. Rise, Matthew L. |
author_facet |
Caballero-Solares, Albert Xue, Xi Cleveland, Beth M. Foroutani, Maryam Beheshti Parrish, Christopher C. Taylor, Richard G. Rise, Matthew L. |
author_sort |
Caballero-Solares, Albert |
title |
Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
title_short |
Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
title_full |
Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
title_fullStr |
Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
title_full_unstemmed |
Diet-Induced Physiological Responses in the Liver of Atlantic Salmon (Salmo salar) Inferred Using Multiplex PCR Platforms |
title_sort |
diet-induced physiological responses in the liver of atlantic salmon (salmo salar) inferred using multiplex pcr platforms |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s10126-020-09972-5 https://link.springer.com/content/pdf/10.1007/s10126-020-09972-5.pdf https://link.springer.com/article/10.1007/s10126-020-09972-5/fulltext.html |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Marine Biotechnology volume 22, issue 4, page 511-525 ISSN 1436-2228 1436-2236 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10126-020-09972-5 |
container_title |
Marine Biotechnology |
container_volume |
22 |
container_issue |
4 |
container_start_page |
511 |
op_container_end_page |
525 |
_version_ |
1766361613446676480 |