Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls
Abstract Objective Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate...
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2020
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crspringernat:10.1007/s10067-020-05502-9 2023-05-15T17:45:08+02:00 Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls Renman, Emma Brink, Mikael Ärlestig, Lisbeth Rantapää-Dahlqvist, Solbritt Lejon, Kristina Vetenskapsrådet Stiftelsen Konung Gustaf V:s 80-årsfond Reumatikerförbundet Umeå Universitet 2020 http://dx.doi.org/10.1007/s10067-020-05502-9 https://link.springer.com/content/pdf/10.1007/s10067-020-05502-9.pdf https://link.springer.com/article/10.1007/s10067-020-05502-9/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Clinical Rheumatology volume 40, issue 6, page 2387-2394 ISSN 0770-3198 1434-9949 General Medicine Rheumatology journal-article 2020 crspringernat https://doi.org/10.1007/s10067-020-05502-9 2022-01-04T13:00:34Z Abstract Objective Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs. Method MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied. Results Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p ( p = 0.020), miR-26b-5p ( p = 0.018), miR-142-3p ( p = 0.005), and miR-155 ( p = 0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression. Conclusions We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed. Key Points • Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls. • In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. Article in Journal/Newspaper Northern Sweden Springer Nature (via Crossref) Clinical Rheumatology 40 6 2387 2394 |
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English |
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General Medicine Rheumatology |
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General Medicine Rheumatology Renman, Emma Brink, Mikael Ärlestig, Lisbeth Rantapää-Dahlqvist, Solbritt Lejon, Kristina Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
topic_facet |
General Medicine Rheumatology |
description |
Abstract Objective Recent studies have demonstrated an altered expression of certain microRNAs in patients with rheumatoid arthritis (RA) as well as their first-degree relatives (FDRs) compared to healthy controls (HCs), suggesting a role of microRNA in the progression of the disease. To corroborate this, a set of well-characterized RA families originating from northern Sweden were analyzed for differential expression of a selected set of microRNAs. Method MicroRNA was isolated from frozen peripheral blood cells obtained from 21 different families and included 26 RA patients, 22 FDRs, and 21 HCs. Expression of the selected microRNAs miR-22-3p, miR-26b-5p, miR-34a-3p, miR-103a-3p, miR-142-3p, miR-146a-5p, miR-155, miR-346, and miR-451a was determined by a two-step quantitative real-time polymerase chain reaction (qRT-PCR). Statistical analysis including clinical variables was applied. Results Out of the nine selected microRNAs that previously have been linked to RA, we confirmed four after adjusting for age and gender, i.e., miR-22-3p ( p = 0.020), miR-26b-5p ( p = 0.018), miR-142-3p ( p = 0.005), and miR-155 ( p = 0.033). Moreover, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. In addition, analysis of the effect of corticosteroid use showed modulation of miR-103a-3p expression. Conclusions We confirm that microRNAs seem to be involved in the development of RA, and that the expression pattern in FDR is partly overlapping with RA patients. The contribution of single microRNAs in relation to the complex network including all microRNAs and other molecules is still to be revealed. Key Points • Expression levels of miR-22-3p, miR-26b-5p, miR-142-3p, and miR-155 were significantly altered in RA patients compared to those in controls. • In first-degree relatives, a significant trend with an intermediate microRNA expression in FDR was observed for the same four microRNAs. |
author2 |
Vetenskapsrådet Stiftelsen Konung Gustaf V:s 80-årsfond Reumatikerförbundet Umeå Universitet |
format |
Article in Journal/Newspaper |
author |
Renman, Emma Brink, Mikael Ärlestig, Lisbeth Rantapää-Dahlqvist, Solbritt Lejon, Kristina |
author_facet |
Renman, Emma Brink, Mikael Ärlestig, Lisbeth Rantapää-Dahlqvist, Solbritt Lejon, Kristina |
author_sort |
Renman, Emma |
title |
Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
title_short |
Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
title_full |
Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
title_fullStr |
Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
title_full_unstemmed |
Dysregulated microRNA expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
title_sort |
dysregulated microrna expression in rheumatoid arthritis families—a comparison between rheumatoid arthritis patients, their first-degree relatives, and healthy controls |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s10067-020-05502-9 https://link.springer.com/content/pdf/10.1007/s10067-020-05502-9.pdf https://link.springer.com/article/10.1007/s10067-020-05502-9/fulltext.html |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_source |
Clinical Rheumatology volume 40, issue 6, page 2387-2394 ISSN 0770-3198 1434-9949 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s10067-020-05502-9 |
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Clinical Rheumatology |
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40 |
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6 |
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2387 |
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2394 |
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1766147915157340160 |