Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us?
Abstract During the last decade, paleoproteomics allowed us to open a direct window into the biological past, improving our understanding of the phylogenetic relationships of extant and extinct species, past human diseases, and reconstruction of the human diet. In particular, meta-proteomic studies,...
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crspringernat:10.1007/s00726-021-03061-0 2023-05-15T18:45:08+02:00 Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? Cucina, Annamaria Cunsolo, Vincenzo Di Francesco, Antonella Saletti, Rosaria Zilberstein, Gleb Zilberstein, Svetlana Tikhonov, Alexei Bublichenko, Andrey G. Righetti, Pier Giorgio Foti, Salvatore University of Catania Università degli Studi di Catania 2021 http://dx.doi.org/10.1007/s00726-021-03061-0 https://link.springer.com/content/pdf/10.1007/s00726-021-03061-0.pdf https://link.springer.com/article/10.1007/s00726-021-03061-0/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Amino Acids volume 53, issue 10, page 1507-1521 ISSN 0939-4451 1438-2199 Organic Chemistry Clinical Biochemistry Biochemistry journal-article 2021 crspringernat https://doi.org/10.1007/s00726-021-03061-0 2022-01-04T10:14:13Z Abstract During the last decade, paleoproteomics allowed us to open a direct window into the biological past, improving our understanding of the phylogenetic relationships of extant and extinct species, past human diseases, and reconstruction of the human diet. In particular, meta-proteomic studies, mainly carried out on ancient human dental calculus, provided insights into past oral microbial communities and ancient diets. On the contrary, very few investigations regard the analysis of ancient gut microbiota, which may enable a greater understanding of how microorganisms and their hosts have co-evolved and spread under the influence of changing diet practices and habitat. In this respect, this paper reports the results of the first-ever meta-proteomic analysis carried out on a gut tissue sample some 40,000 years old. Proteins were extracted by applying EVA (ethylene–vinyl acetate) films to the surface of the gut sample of a woolly mammoth ( Mammuthus primigenus ), discovered in 1972 close to the Shandrin River (Yakutia, Russia), and then investigated via a shotgun MS-based approach. Proteomic and peptidomic analysis allowed in-depth exploration of its meta-proteome composition. The results were validated through the level of deamidation and other diagenetic chemical modifications of the sample peptides, which were used to discriminate the “original” endogenous peptides from contaminant ones. Overall, the results of the meta-proteomic analysis here reported agreeing with the previous paleobotanical studies and with the reconstructed habitat of the Shandrin mammoth and provided insight into its diet. The data have been deposited to the ProteomeXchange with identifier < PXD025518 > . Article in Journal/Newspaper Yakutia Springer Nature (via Crossref) Shandrin ENVELOPE(151.056,151.056,70.922,70.922) Amino Acids |
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Springer Nature (via Crossref) |
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English |
topic |
Organic Chemistry Clinical Biochemistry Biochemistry |
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Organic Chemistry Clinical Biochemistry Biochemistry Cucina, Annamaria Cunsolo, Vincenzo Di Francesco, Antonella Saletti, Rosaria Zilberstein, Gleb Zilberstein, Svetlana Tikhonov, Alexei Bublichenko, Andrey G. Righetti, Pier Giorgio Foti, Salvatore Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
topic_facet |
Organic Chemistry Clinical Biochemistry Biochemistry |
description |
Abstract During the last decade, paleoproteomics allowed us to open a direct window into the biological past, improving our understanding of the phylogenetic relationships of extant and extinct species, past human diseases, and reconstruction of the human diet. In particular, meta-proteomic studies, mainly carried out on ancient human dental calculus, provided insights into past oral microbial communities and ancient diets. On the contrary, very few investigations regard the analysis of ancient gut microbiota, which may enable a greater understanding of how microorganisms and their hosts have co-evolved and spread under the influence of changing diet practices and habitat. In this respect, this paper reports the results of the first-ever meta-proteomic analysis carried out on a gut tissue sample some 40,000 years old. Proteins were extracted by applying EVA (ethylene–vinyl acetate) films to the surface of the gut sample of a woolly mammoth ( Mammuthus primigenus ), discovered in 1972 close to the Shandrin River (Yakutia, Russia), and then investigated via a shotgun MS-based approach. Proteomic and peptidomic analysis allowed in-depth exploration of its meta-proteome composition. The results were validated through the level of deamidation and other diagenetic chemical modifications of the sample peptides, which were used to discriminate the “original” endogenous peptides from contaminant ones. Overall, the results of the meta-proteomic analysis here reported agreeing with the previous paleobotanical studies and with the reconstructed habitat of the Shandrin mammoth and provided insight into its diet. The data have been deposited to the ProteomeXchange with identifier < PXD025518 > . |
author2 |
University of Catania Università degli Studi di Catania |
format |
Article in Journal/Newspaper |
author |
Cucina, Annamaria Cunsolo, Vincenzo Di Francesco, Antonella Saletti, Rosaria Zilberstein, Gleb Zilberstein, Svetlana Tikhonov, Alexei Bublichenko, Andrey G. Righetti, Pier Giorgio Foti, Salvatore |
author_facet |
Cucina, Annamaria Cunsolo, Vincenzo Di Francesco, Antonella Saletti, Rosaria Zilberstein, Gleb Zilberstein, Svetlana Tikhonov, Alexei Bublichenko, Andrey G. Righetti, Pier Giorgio Foti, Salvatore |
author_sort |
Cucina, Annamaria |
title |
Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
title_short |
Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
title_full |
Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
title_fullStr |
Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
title_full_unstemmed |
Meta-proteomic analysis of the Shandrin mammoth by EVA technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
title_sort |
meta-proteomic analysis of the shandrin mammoth by eva technology and high-resolution mass spectrometry: what is its gut microbiota telling us? |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1007/s00726-021-03061-0 https://link.springer.com/content/pdf/10.1007/s00726-021-03061-0.pdf https://link.springer.com/article/10.1007/s00726-021-03061-0/fulltext.html |
long_lat |
ENVELOPE(151.056,151.056,70.922,70.922) |
geographic |
Shandrin |
geographic_facet |
Shandrin |
genre |
Yakutia |
genre_facet |
Yakutia |
op_source |
Amino Acids volume 53, issue 10, page 1507-1521 ISSN 0939-4451 1438-2199 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00726-021-03061-0 |
container_title |
Amino Acids |
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1766236098419228672 |