Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas
Abstract Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid–base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial...
Published in: | Journal of Comparative Physiology B |
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Format: | Article in Journal/Newspaper |
Language: | English |
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Springer Science and Business Media LLC
2020
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Online Access: | http://dx.doi.org/10.1007/s00360-020-01303-3 https://link.springer.com/content/pdf/10.1007/s00360-020-01303-3.pdf https://link.springer.com/article/10.1007/s00360-020-01303-3/fulltext.html |
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crspringernat:10.1007/s00360-020-01303-3 2023-05-15T15:57:48+02:00 Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas Ramesh, Kirti Hu, Marian Y. Melzner, Frank Bleich, Markus Himmerkus, Nina Seventh Framework Programme Emmy-Noether Program University of Gothenburg 2020 http://dx.doi.org/10.1007/s00360-020-01303-3 https://link.springer.com/content/pdf/10.1007/s00360-020-01303-3.pdf https://link.springer.com/article/10.1007/s00360-020-01303-3/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Journal of Comparative Physiology B volume 190, issue 6, page 691-700 ISSN 0174-1578 1432-136X Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology journal-article 2020 crspringernat https://doi.org/10.1007/s00360-020-01303-3 2022-01-04T12:06:31Z Abstract Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid–base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial cells from the Pacific oyster, Crassostrea gigas and utilise live cell imaging in combination with the fluorescent dye, BCECF-AM to study intracellular pH (pH i ) regulation. To elucidate the involvement of various ion transport mechanisms, modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid–base proteins were used. Diminished pH recovery in the absence of Na + and under inhibition of sodium/hydrogen exchangers (NHEs) implicate the involvement of a sodium dependent cellular proton extrusion mechanism. In addition, pH recovery was reduced under inhibition of carbonic anhydrases. These data provide the foundation for a better understanding of acid–base regulation underlying the physiology of calcification in bivalves. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Springer Nature (via Crossref) Pacific Journal of Comparative Physiology B 190 6 691 700 |
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Open Polar |
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Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology |
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Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology Ramesh, Kirti Hu, Marian Y. Melzner, Frank Bleich, Markus Himmerkus, Nina Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
topic_facet |
Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology |
description |
Abstract Shell formation and repair occurs under the control of mantle epithelial cells in bivalve molluscs. However, limited information is available on the precise acid–base regulatory machinery present within these cells, which are fundamental to calcification. Here, we isolate mantle epithelial cells from the Pacific oyster, Crassostrea gigas and utilise live cell imaging in combination with the fluorescent dye, BCECF-AM to study intracellular pH (pH i ) regulation. To elucidate the involvement of various ion transport mechanisms, modified seawater solutions (low sodium, low bicarbonate) and specific inhibitors for acid–base proteins were used. Diminished pH recovery in the absence of Na + and under inhibition of sodium/hydrogen exchangers (NHEs) implicate the involvement of a sodium dependent cellular proton extrusion mechanism. In addition, pH recovery was reduced under inhibition of carbonic anhydrases. These data provide the foundation for a better understanding of acid–base regulation underlying the physiology of calcification in bivalves. |
author2 |
Seventh Framework Programme Emmy-Noether Program University of Gothenburg |
format |
Article in Journal/Newspaper |
author |
Ramesh, Kirti Hu, Marian Y. Melzner, Frank Bleich, Markus Himmerkus, Nina |
author_facet |
Ramesh, Kirti Hu, Marian Y. Melzner, Frank Bleich, Markus Himmerkus, Nina |
author_sort |
Ramesh, Kirti |
title |
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
title_short |
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
title_full |
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
title_fullStr |
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
title_full_unstemmed |
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas |
title_sort |
intracellular ph regulation in mantle epithelial cells of the pacific oyster, crassostrea gigas |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s00360-020-01303-3 https://link.springer.com/content/pdf/10.1007/s00360-020-01303-3.pdf https://link.springer.com/article/10.1007/s00360-020-01303-3/fulltext.html |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Journal of Comparative Physiology B volume 190, issue 6, page 691-700 ISSN 0174-1578 1432-136X |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00360-020-01303-3 |
container_title |
Journal of Comparative Physiology B |
container_volume |
190 |
container_issue |
6 |
container_start_page |
691 |
op_container_end_page |
700 |
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1766393507295002624 |