Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species
Abstract Inward rectifier K + (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod ( Boreogadus saida ) and navaga ( Eleginus nawaga ) of the Arctic Ocean and burbot ( Lota lota...
Published in: | Journal of Comparative Physiology B |
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Online Access: | http://dx.doi.org/10.1007/s00360-019-01241-9 http://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://link.springer.com/article/10.1007/s00360-019-01241-9/fulltext.html |
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crspringernat:10.1007/s00360-019-01241-9 2023-05-15T14:54:53+02:00 Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species Hassinen, Minna Korajoki, Hanna Abramochkin, Denis Krivosheya, Pavel Vornanen, Matti Academy of Finland 2019 http://dx.doi.org/10.1007/s00360-019-01241-9 http://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://link.springer.com/article/10.1007/s00360-019-01241-9/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Journal of Comparative Physiology B volume 189, issue 6, page 735-749 ISSN 0174-1578 1432-136X Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology journal-article 2019 crspringernat https://doi.org/10.1007/s00360-019-01241-9 2022-01-04T16:08:25Z Abstract Inward rectifier K + (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod ( Boreogadus saida ) and navaga ( Eleginus nawaga ) of the Arctic Ocean and burbot ( Lota lota ) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout ( Salmo trutta fario ), arctic char ( Salvelinus alpinus ), roach ( Rutilus rutilus ), perch ( Perca fluviatilis ) and pike ( Esox lucius ), and zebrafish ( Danio rerio ), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. Article in Journal/Newspaper Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota Springer Nature (via Crossref) Arctic Arctic Ocean Navaga ENVELOPE(162.106,162.106,59.229,59.229) Journal of Comparative Physiology B 189 6 735 749 |
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Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology |
spellingShingle |
Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology Hassinen, Minna Korajoki, Hanna Abramochkin, Denis Krivosheya, Pavel Vornanen, Matti Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
topic_facet |
Endocrinology Animal Science and Zoology Biochemistry Ecology, Evolution, Behavior and Systematics Physiology |
description |
Abstract Inward rectifier K + (Kir2) channels are critical for electrical excitability of cardiac myocytes. Here, we examine expression of Kir2 channels in the heart of three Gadiformes species, polar cod ( Boreogadus saida ) and navaga ( Eleginus nawaga ) of the Arctic Ocean and burbot ( Lota lota ) of the temperate lakes to find out the role of Kir2 channels in cardiac adaptation to cold. Five boreal freshwater species: brown trout ( Salmo trutta fario ), arctic char ( Salvelinus alpinus ), roach ( Rutilus rutilus ), perch ( Perca fluviatilis ) and pike ( Esox lucius ), and zebrafish ( Danio rerio ), were included for comparison. Transcript expression of genes encoding Kir2.1a, − 2.1b, − 2.2a, − 2.2b and − 2.4 was studied from atrium and ventricle of thermally acclimated or acclimatized fish by quantitative PCR. Kir2 composition in the polar cod was more diverse than in other species in that all Kir2 isoforms were relatively highly expressed. Kir2 composition of navaga and burbot differed from that of the polar cod as well as from those of other species. The relative expression of Kir2.2 transcripts, especially Kir2.2b, was higher in both atrium and ventricle of navaga and burbot (56–89% from the total Kir2 pool) than in other species (0.1–11%). Thermal acclimation induced only small changes in cardiac Kir2 transcript expression in Gadiformes species. However, Kir2.2b transcripts were upregulated in cold-acclimated navaga and burbot hearts. All in all, the cardiac Kir2 composition seems to be dependent on both phylogenetic position and thermal preference of the fish. |
author2 |
Academy of Finland |
format |
Article in Journal/Newspaper |
author |
Hassinen, Minna Korajoki, Hanna Abramochkin, Denis Krivosheya, Pavel Vornanen, Matti |
author_facet |
Hassinen, Minna Korajoki, Hanna Abramochkin, Denis Krivosheya, Pavel Vornanen, Matti |
author_sort |
Hassinen, Minna |
title |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_short |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_fullStr |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_full_unstemmed |
Transcript expression of inward rectifier potassium channels of Kir2 subfamily in Arctic marine and freshwater fish species |
title_sort |
transcript expression of inward rectifier potassium channels of kir2 subfamily in arctic marine and freshwater fish species |
publisher |
Springer Science and Business Media LLC |
publishDate |
2019 |
url |
http://dx.doi.org/10.1007/s00360-019-01241-9 http://link.springer.com/content/pdf/10.1007/s00360-019-01241-9.pdf http://link.springer.com/article/10.1007/s00360-019-01241-9/fulltext.html |
long_lat |
ENVELOPE(162.106,162.106,59.229,59.229) |
geographic |
Arctic Arctic Ocean Navaga |
geographic_facet |
Arctic Arctic Ocean Navaga |
genre |
Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
genre_facet |
Arctic Arctic Ocean Boreogadus saida Burbot Esox lucius Lota lota polar cod Salvelinus alpinus lota |
op_source |
Journal of Comparative Physiology B volume 189, issue 6, page 735-749 ISSN 0174-1578 1432-136X |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00360-019-01241-9 |
container_title |
Journal of Comparative Physiology B |
container_volume |
189 |
container_issue |
6 |
container_start_page |
735 |
op_container_end_page |
749 |
_version_ |
1766326630957973504 |