Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex
Abstract In the Southern Ocean (SO), iron (Fe) limitation strongly inhibits phytoplankton growth and generally decreases their primary productivity. Diatoms are a key component in the carbon (C) cycle, by taking up large amounts of anthropogenic CO 2 through the biological carbon pump. In this study...
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2021
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crspringernat:10.1007/s00300-020-02785-1 2023-05-15T14:07:05+02:00 Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex Bozzato, Deborah Jakob, Torsten Wilhelm, Christian Trimborn, Scarlett Deutsche Forschungsgemeinschaft HGF Young Investigators Group EcoTrace 2021 http://dx.doi.org/10.1007/s00300-020-02785-1 http://link.springer.com/content/pdf/10.1007/s00300-020-02785-1.pdf http://link.springer.com/article/10.1007/s00300-020-02785-1/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Polar Biology volume 44, issue 2, page 275-287 ISSN 0722-4060 1432-2056 General Agricultural and Biological Sciences journal-article 2021 crspringernat https://doi.org/10.1007/s00300-020-02785-1 2022-01-04T08:33:11Z Abstract In the Southern Ocean (SO), iron (Fe) limitation strongly inhibits phytoplankton growth and generally decreases their primary productivity. Diatoms are a key component in the carbon (C) cycle, by taking up large amounts of anthropogenic CO 2 through the biological carbon pump. In this study, we investigated the effects of Fe availability (no Fe and 4 nM FeCl 3 addition) on the physiology of Chaetoceros cf. simplex , an ecologically relevant SO diatom. Our results are the first combining oxygen evolution and uptake rates with particulate organic carbon (POC) build up, pigments, photophysiological parameters and intracellular trace metal (TM) quotas in an Fe-deficient Antarctic diatom. Decreases in both oxygen evolution (through photosynthesis, P) and uptake (respiration, R) coincided with a lowered growth rate of Fe-deficient cells. In addition, cells displayed reduced electron transport rates (ETR) and chlorophyll a (Chla) content, resulting in reduced cellular POC formation. Interestingly, no differences were observed in non-photochemical quenching (NPQ) or in the ratio of gross photosynthesis to respiration (GP:R). Furthermore, TM quotas were measured, which represent an important and rarely quantified parameter in previous studies. Cellular quotas of manganese, zinc, cobalt and copper remained unchanged while Fe quotas of Fe-deficient cells were reduced by 60% compared with High Fe cells. Based on our data, Fe-deficient Chaetoceros cf. simplex cells were able to efficiently acclimate to low Fe conditions, reducing their intracellular Fe concentrations, the number of functional reaction centers of photosystem II (RCII) and photosynthetic rates, thus avoiding light absorption rather than dissipating the energy through NPQ. Our results demonstrate how Chaetoceros cf. simplex can adapt their physiology to lowered assimilatory metabolism by decreasing respiratory losses. Article in Journal/Newspaper Antarc* Antarctic Polar Biology Southern Ocean Springer Nature (via Crossref) Antarctic Southern Ocean The Antarctic Polar Biology 44 2 275 287 |
institution |
Open Polar |
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Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
General Agricultural and Biological Sciences |
spellingShingle |
General Agricultural and Biological Sciences Bozzato, Deborah Jakob, Torsten Wilhelm, Christian Trimborn, Scarlett Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
topic_facet |
General Agricultural and Biological Sciences |
description |
Abstract In the Southern Ocean (SO), iron (Fe) limitation strongly inhibits phytoplankton growth and generally decreases their primary productivity. Diatoms are a key component in the carbon (C) cycle, by taking up large amounts of anthropogenic CO 2 through the biological carbon pump. In this study, we investigated the effects of Fe availability (no Fe and 4 nM FeCl 3 addition) on the physiology of Chaetoceros cf. simplex , an ecologically relevant SO diatom. Our results are the first combining oxygen evolution and uptake rates with particulate organic carbon (POC) build up, pigments, photophysiological parameters and intracellular trace metal (TM) quotas in an Fe-deficient Antarctic diatom. Decreases in both oxygen evolution (through photosynthesis, P) and uptake (respiration, R) coincided with a lowered growth rate of Fe-deficient cells. In addition, cells displayed reduced electron transport rates (ETR) and chlorophyll a (Chla) content, resulting in reduced cellular POC formation. Interestingly, no differences were observed in non-photochemical quenching (NPQ) or in the ratio of gross photosynthesis to respiration (GP:R). Furthermore, TM quotas were measured, which represent an important and rarely quantified parameter in previous studies. Cellular quotas of manganese, zinc, cobalt and copper remained unchanged while Fe quotas of Fe-deficient cells were reduced by 60% compared with High Fe cells. Based on our data, Fe-deficient Chaetoceros cf. simplex cells were able to efficiently acclimate to low Fe conditions, reducing their intracellular Fe concentrations, the number of functional reaction centers of photosystem II (RCII) and photosynthetic rates, thus avoiding light absorption rather than dissipating the energy through NPQ. Our results demonstrate how Chaetoceros cf. simplex can adapt their physiology to lowered assimilatory metabolism by decreasing respiratory losses. |
author2 |
Deutsche Forschungsgemeinschaft HGF Young Investigators Group EcoTrace |
format |
Article in Journal/Newspaper |
author |
Bozzato, Deborah Jakob, Torsten Wilhelm, Christian Trimborn, Scarlett |
author_facet |
Bozzato, Deborah Jakob, Torsten Wilhelm, Christian Trimborn, Scarlett |
author_sort |
Bozzato, Deborah |
title |
Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
title_short |
Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
title_full |
Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
title_fullStr |
Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
title_full_unstemmed |
Effects of iron limitation on carbon balance and photophysiology of the Antarctic diatom Chaetoceros cf. simplex |
title_sort |
effects of iron limitation on carbon balance and photophysiology of the antarctic diatom chaetoceros cf. simplex |
publisher |
Springer Science and Business Media LLC |
publishDate |
2021 |
url |
http://dx.doi.org/10.1007/s00300-020-02785-1 http://link.springer.com/content/pdf/10.1007/s00300-020-02785-1.pdf http://link.springer.com/article/10.1007/s00300-020-02785-1/fulltext.html |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Polar Biology Southern Ocean |
genre_facet |
Antarc* Antarctic Polar Biology Southern Ocean |
op_source |
Polar Biology volume 44, issue 2, page 275-287 ISSN 0722-4060 1432-2056 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00300-020-02785-1 |
container_title |
Polar Biology |
container_volume |
44 |
container_issue |
2 |
container_start_page |
275 |
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287 |
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1766279001191481344 |