Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance
Abstract Despite cold adaptation, Antarctic fish show lower growth than expected from the van’t Hoff’s Q 10 rule. Protein synthesis is one of the main energy-consuming processes, which is downregulated under energy deficiency. Considering the effect of temperature on growth performance, we tested if...
Published in: | Polar Biology |
---|---|
Main Authors: | , , , |
Other Authors: | |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Springer Science and Business Media LLC
2020
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1007/s00300-020-02752-w https://link.springer.com/content/pdf/10.1007/s00300-020-02752-w.pdf https://link.springer.com/article/10.1007/s00300-020-02752-w/fulltext.html |
id |
crspringernat:10.1007/s00300-020-02752-w |
---|---|
record_format |
openpolar |
spelling |
crspringernat:10.1007/s00300-020-02752-w 2023-05-15T14:06:02+02:00 Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance Lannig, Gisela Tillmann, Anette Howald, Sarah Stapp, Laura S. Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) 2020 http://dx.doi.org/10.1007/s00300-020-02752-w https://link.springer.com/content/pdf/10.1007/s00300-020-02752-w.pdf https://link.springer.com/article/10.1007/s00300-020-02752-w/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Polar Biology volume 43, issue 11, page 1887-1898 ISSN 0722-4060 1432-2056 General Agricultural and Biological Sciences journal-article 2020 crspringernat https://doi.org/10.1007/s00300-020-02752-w 2022-01-04T11:40:13Z Abstract Despite cold adaptation, Antarctic fish show lower growth than expected from the van’t Hoff’s Q 10 rule. Protein synthesis is one of the main energy-consuming processes, which is downregulated under energy deficiency. Considering the effect of temperature on growth performance, we tested if temperature-dependent cellular energy allocation to protein synthesis correlates with temperature-dependent whole-animal growth and thus thermal tolerance. Cell respiration and energy expenditure for protein synthesis were determined in hepatocytes of the circumpolar-distributed Antarctic eelpout Pachycara brachycephalum after warm acclimation (0 °C vs 5 °C) and, of two notothenioids the sub-Antarctic Lepidonotothen squamifrons and the high-Antarctic icefish Chionodraco hamatus . We used intermittent-flow respirometry to analyse cellular response to acute warming from 5 to 10 °C ( P. brachycephalum ) and from 1 to 5 °C ( L. squamifrons, C. hamatus ). Warming-induced rise in respiration was similar between 0- and 5 °C-acclimated P. brachycephalum and between L. squamifrons and C. hamatus . Irrespective of acclimation, warming decreased energy expenditure for protein synthesis in P. brachycephalum , which corresponds to reduced whole-animal growth at temperatures > 5 °C. Warming doubled energy expenditure for protein synthesis in L. squamifrons but had no effect on C. hamatus indicating that L. squamifrons might benefit from warmer waters. The species-specific temperature effect on energy expenditure for protein synthesis is discussed to mirror thermal sensitivity of whole-animal growth performance, thereby paralleling the degree of cold adaptation. Clearly more data are necessary including measurements at narrower temperature steps particularly for C. hamatus and an increased species’ number per ecotype to reinforce presented link between cellular and whole-animal thermal sensitivity. Article in Journal/Newspaper Antarc* Antarctic Icefish Polar Biology Springer Nature (via Crossref) Antarctic Polar Biology 43 11 1887 1898 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
General Agricultural and Biological Sciences |
spellingShingle |
General Agricultural and Biological Sciences Lannig, Gisela Tillmann, Anette Howald, Sarah Stapp, Laura S. Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
topic_facet |
General Agricultural and Biological Sciences |
description |
Abstract Despite cold adaptation, Antarctic fish show lower growth than expected from the van’t Hoff’s Q 10 rule. Protein synthesis is one of the main energy-consuming processes, which is downregulated under energy deficiency. Considering the effect of temperature on growth performance, we tested if temperature-dependent cellular energy allocation to protein synthesis correlates with temperature-dependent whole-animal growth and thus thermal tolerance. Cell respiration and energy expenditure for protein synthesis were determined in hepatocytes of the circumpolar-distributed Antarctic eelpout Pachycara brachycephalum after warm acclimation (0 °C vs 5 °C) and, of two notothenioids the sub-Antarctic Lepidonotothen squamifrons and the high-Antarctic icefish Chionodraco hamatus . We used intermittent-flow respirometry to analyse cellular response to acute warming from 5 to 10 °C ( P. brachycephalum ) and from 1 to 5 °C ( L. squamifrons, C. hamatus ). Warming-induced rise in respiration was similar between 0- and 5 °C-acclimated P. brachycephalum and between L. squamifrons and C. hamatus . Irrespective of acclimation, warming decreased energy expenditure for protein synthesis in P. brachycephalum , which corresponds to reduced whole-animal growth at temperatures > 5 °C. Warming doubled energy expenditure for protein synthesis in L. squamifrons but had no effect on C. hamatus indicating that L. squamifrons might benefit from warmer waters. The species-specific temperature effect on energy expenditure for protein synthesis is discussed to mirror thermal sensitivity of whole-animal growth performance, thereby paralleling the degree of cold adaptation. Clearly more data are necessary including measurements at narrower temperature steps particularly for C. hamatus and an increased species’ number per ecotype to reinforce presented link between cellular and whole-animal thermal sensitivity. |
author2 |
Alfred-Wegener-Institut, Helmholtz-Zentrum für Polar- und Meeresforschung (AWI) |
format |
Article in Journal/Newspaper |
author |
Lannig, Gisela Tillmann, Anette Howald, Sarah Stapp, Laura S. |
author_facet |
Lannig, Gisela Tillmann, Anette Howald, Sarah Stapp, Laura S. |
author_sort |
Lannig, Gisela |
title |
Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
title_short |
Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
title_full |
Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
title_fullStr |
Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
title_full_unstemmed |
Thermal sensitivity of cell metabolism of different Antarctic fish species mirrors organism temperature tolerance |
title_sort |
thermal sensitivity of cell metabolism of different antarctic fish species mirrors organism temperature tolerance |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s00300-020-02752-w https://link.springer.com/content/pdf/10.1007/s00300-020-02752-w.pdf https://link.springer.com/article/10.1007/s00300-020-02752-w/fulltext.html |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Icefish Polar Biology |
genre_facet |
Antarc* Antarctic Icefish Polar Biology |
op_source |
Polar Biology volume 43, issue 11, page 1887-1898 ISSN 0722-4060 1432-2056 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00300-020-02752-w |
container_title |
Polar Biology |
container_volume |
43 |
container_issue |
11 |
container_start_page |
1887 |
op_container_end_page |
1898 |
_version_ |
1766277797098029056 |