Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery
Abstract Ballan wrasse ( Labrus bergylta , Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon ( Salmo salar , Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleos...
Published in: | Current Microbiology |
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Springer Science and Business Media LLC
2020
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Online Access: | http://dx.doi.org/10.1007/s00284-020-02286-8 http://link.springer.com/content/pdf/10.1007/s00284-020-02286-8.pdf http://link.springer.com/article/10.1007/s00284-020-02286-8/fulltext.html |
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crspringernat:10.1007/s00284-020-02286-8 2023-05-15T15:32:59+02:00 Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P. University of Stirling 2020 http://dx.doi.org/10.1007/s00284-020-02286-8 http://link.springer.com/content/pdf/10.1007/s00284-020-02286-8.pdf http://link.springer.com/article/10.1007/s00284-020-02286-8/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Current Microbiology volume 78, issue 1, page 114-124 ISSN 0343-8651 1432-0991 Applied Microbiology and Biotechnology General Medicine Microbiology journal-article 2020 crspringernat https://doi.org/10.1007/s00284-020-02286-8 2022-01-04T15:49:07Z Abstract Ballan wrasse ( Labrus bergylta , Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon ( Salmo salar , Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs. Article in Journal/Newspaper Atlantic salmon Salmo salar Springer Nature (via Crossref) Ballan ENVELOPE(12.203,12.203,65.945,65.945) Current Microbiology 78 1 114 124 |
institution |
Open Polar |
collection |
Springer Nature (via Crossref) |
op_collection_id |
crspringernat |
language |
English |
topic |
Applied Microbiology and Biotechnology General Medicine Microbiology |
spellingShingle |
Applied Microbiology and Biotechnology General Medicine Microbiology Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P. Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
topic_facet |
Applied Microbiology and Biotechnology General Medicine Microbiology |
description |
Abstract Ballan wrasse ( Labrus bergylta , Ascanius 1767) are cleaner fish cultured in northern Europe to remove sea lice from farmed Atlantic salmon ( Salmo salar , Linnaeus 1758). Despite increasing appreciation for the importance of the microbiota on the phenotypes of vertebrates including teleosts, the microbiota of wrasse eggs has yet to be described. Therefore, the aim of this present study was to describe the bacterial component of the microbiota of ballan wrasse eggs shortly after spawning and at 5 days, once the eggs had undergone a routine incubation protocol that included surface disinfection steps in a common holding tank. Triplicate egg samples were collected from each of three spawning tanks and analysis of 16S rRNA gene sequences revealed that 88.6% of reads could be identified to 186 taxonomic families. At Day 0, reads corresponding to members of the Vibrionaceae, Colwelliaceae and Rubritaleaceae families were detected at greatest relative abundances. Bacterial communities of eggs varied more greatly between tanks than between samples deriving from the same tank. At Day 5, there was a consistent reduction in 16S rRNA gene sequence richness across the tanks. Even though the eggs from the different tanks were incubated in a common holding tank, the bacterial communities of the eggs from the different tanks had diverged to become increasingly dissimilar. This suggests that the disinfection and incubation exerted differential effects of the microbiota of the eggs from each tank and that the influence of the tank water on the composition of the egg microbiota was lower than expected. This first comprehensive description of the ballan wrasse egg bacterial community is an initial step to understand the role and function of the microbiota on the phenotype of this fish. In future, mass DNA sequencing methods may be applied in hatcheries to screen for pathogens and as a tool to assess the health status of eggs. |
author2 |
University of Stirling |
format |
Article in Journal/Newspaper |
author |
Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P. |
author_facet |
Bone, Aileen Bekaert, Michaël Papadopoulou, Athina McMillan, Stuart Adams, Alexandra Davie, Andrew Desbois, Andrew P. |
author_sort |
Bone, Aileen |
title |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_short |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_full |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_fullStr |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_full_unstemmed |
Bacterial Communities of Ballan Wrasse (Labrus bergylta) Eggs at a Commercial Marine Hatchery |
title_sort |
bacterial communities of ballan wrasse (labrus bergylta) eggs at a commercial marine hatchery |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s00284-020-02286-8 http://link.springer.com/content/pdf/10.1007/s00284-020-02286-8.pdf http://link.springer.com/article/10.1007/s00284-020-02286-8/fulltext.html |
long_lat |
ENVELOPE(12.203,12.203,65.945,65.945) |
geographic |
Ballan |
geographic_facet |
Ballan |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Current Microbiology volume 78, issue 1, page 114-124 ISSN 0343-8651 1432-0991 |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00284-020-02286-8 |
container_title |
Current Microbiology |
container_volume |
78 |
container_issue |
1 |
container_start_page |
114 |
op_container_end_page |
124 |
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1766363465389178880 |