Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria
Abstract Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial b...
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Springer Science and Business Media LLC
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crspringernat:10.1007/s00248-020-01614-6 2023-05-15T14:06:02+02:00 Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria Baeza, Nicolas Mercade, Elena Ministerio de Ciencia, Innovación y Universidades Departament d'Innovació, Universitats i Empresa, Generalitat de Catalunya 2020 http://dx.doi.org/10.1007/s00248-020-01614-6 https://link.springer.com/content/pdf/10.1007/s00248-020-01614-6.pdf https://link.springer.com/article/10.1007/s00248-020-01614-6/fulltext.html en eng Springer Science and Business Media LLC https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 CC-BY Microbial Ecology volume 81, issue 3, page 645-656 ISSN 0095-3628 1432-184X Soil Science Ecology Ecology, Evolution, Behavior and Systematics journal-article 2020 crspringernat https://doi.org/10.1007/s00248-020-01614-6 2022-01-04T15:36:19Z Abstract Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial behavior and control biofilm formation when necessary. We used a collection of cold-adapted Antarctic Gram-negative bacteria to study whether their ability to form biofilms is associated with a capacity to produce membrane vesicles and secrete extracellular ATP. In most of the studied strains, no correlation was found between biofilm formation and these two factors. Only Shewanella vesiculosa M7 T secreted high levels of extracellular ATP, and its membrane vesicles caused a significant increase in the speed and amount of biofilm formation. In this strain, an important portion of the exogenous ATP was contained in membrane vesicles, where it was protected from apyrase treatment. These results confirm that ATP influences biofilm formation. Although the role of extracellular ATP in prokaryotes is still not well understood, the metabolic cost of its production suggests it has an important function, such as a role in biofilm formation. Thus, the liberation of extracellular ATP through membrane vesicles and its function deserve further study. Article in Journal/Newspaper Antarc* Antarctic Springer Nature (via Crossref) Antarctic Microbial Ecology 81 3 645 656 |
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Open Polar |
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Springer Nature (via Crossref) |
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crspringernat |
language |
English |
topic |
Soil Science Ecology Ecology, Evolution, Behavior and Systematics |
spellingShingle |
Soil Science Ecology Ecology, Evolution, Behavior and Systematics Baeza, Nicolas Mercade, Elena Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
topic_facet |
Soil Science Ecology Ecology, Evolution, Behavior and Systematics |
description |
Abstract Biofilms offer a safe environment that favors bacterial survival; for this reason, most pathogenic and environmental bacteria live integrated in biofilm communities. The development of biofilms is complex and involves many factors, which need to be studied in order to understand bacterial behavior and control biofilm formation when necessary. We used a collection of cold-adapted Antarctic Gram-negative bacteria to study whether their ability to form biofilms is associated with a capacity to produce membrane vesicles and secrete extracellular ATP. In most of the studied strains, no correlation was found between biofilm formation and these two factors. Only Shewanella vesiculosa M7 T secreted high levels of extracellular ATP, and its membrane vesicles caused a significant increase in the speed and amount of biofilm formation. In this strain, an important portion of the exogenous ATP was contained in membrane vesicles, where it was protected from apyrase treatment. These results confirm that ATP influences biofilm formation. Although the role of extracellular ATP in prokaryotes is still not well understood, the metabolic cost of its production suggests it has an important function, such as a role in biofilm formation. Thus, the liberation of extracellular ATP through membrane vesicles and its function deserve further study. |
author2 |
Ministerio de Ciencia, Innovación y Universidades Departament d'Innovació, Universitats i Empresa, Generalitat de Catalunya |
format |
Article in Journal/Newspaper |
author |
Baeza, Nicolas Mercade, Elena |
author_facet |
Baeza, Nicolas Mercade, Elena |
author_sort |
Baeza, Nicolas |
title |
Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
title_short |
Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
title_full |
Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
title_fullStr |
Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
title_full_unstemmed |
Relationship Between Membrane Vesicles, Extracellular ATP and Biofilm Formation in Antarctic Gram-Negative Bacteria |
title_sort |
relationship between membrane vesicles, extracellular atp and biofilm formation in antarctic gram-negative bacteria |
publisher |
Springer Science and Business Media LLC |
publishDate |
2020 |
url |
http://dx.doi.org/10.1007/s00248-020-01614-6 https://link.springer.com/content/pdf/10.1007/s00248-020-01614-6.pdf https://link.springer.com/article/10.1007/s00248-020-01614-6/fulltext.html |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Microbial Ecology volume 81, issue 3, page 645-656 ISSN 0095-3628 1432-184X |
op_rights |
https://creativecommons.org/licenses/by/4.0 https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.1007/s00248-020-01614-6 |
container_title |
Microbial Ecology |
container_volume |
81 |
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3 |
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645 |
op_container_end_page |
656 |
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1766277790372462592 |