Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae
The bush dog (Speothos venaticus, 2n = 74) is a near threatened species taxonomically classified among South American canids. We revised the bush dog karyotype and performed a comparative sequence analysis of satellite and satellite-like DNAs in 6 canids: the bush dog, domestic dog (Canis familiaris...
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Online Access: | http://dx.doi.org/10.1159/000503082 https://karger.com/cgr/article-pdf/159/2/88/3897439/000503082.pdf |
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crskarger:10.1159/000503082 2024-06-09T07:42:47+00:00 Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Fröhlich, Jan Vodicka, Roman Rubes, Jiri 2019 http://dx.doi.org/10.1159/000503082 https://karger.com/cgr/article-pdf/159/2/88/3897439/000503082.pdf en eng S. Karger AG https://karger.com/pages/terms-and-conditions https://karger.com/pages/terms-and-conditions Cytogenetic and Genome Research volume 159, issue 2, page 88-96 ISSN 1424-8581 1424-859X journal-article 2019 crskarger https://doi.org/10.1159/000503082 2024-05-15T13:29:44Z The bush dog (Speothos venaticus, 2n = 74) is a near threatened species taxonomically classified among South American canids. We revised the bush dog karyotype and performed a comparative sequence analysis of satellite and satellite-like DNAs in 6 canids: the bush dog, domestic dog (Canis familiaris, 2n = 78), grey wolf (C. lupus, 2n = 78), Chinese raccoon dog (Nyctereutes procyonoides procyonoides, 2n = 54+B), red fox (Vulpes vulpes, 2n = 34+B), and arctic fox (V. lagopus, 2n = 48-50) to specify the species position among Canidae. Using FISH with painting and BAC probes, we found that the distribution of canid evolutionarily conserved chromosome segments in the bush dog karyotype is similar to that of the domestic dog and grey wolf. The bush dog karyotype differs by 2 acrocentric chromosome pairs formed by tandem fusions of the canine (29;34) and (26;35) orthologues. An interstitial signal of the telomeric probe was observed in the (26;35) fusion site in the bush dog indicating a recent evolutionary origin of this rearrangement. Sequences and hybridisation patterns of satellite DNAs were compared, and a phylogenetic tree of the 6 canid species was constructed which confirmed the bush dog position close to the wolf-like canids, and apart from the raccoon dog and foxes. Article in Journal/Newspaper Arctic Fox Arctic Karger Arctic Cytogenetic and Genome Research 159 2 88 96 |
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English |
description |
The bush dog (Speothos venaticus, 2n = 74) is a near threatened species taxonomically classified among South American canids. We revised the bush dog karyotype and performed a comparative sequence analysis of satellite and satellite-like DNAs in 6 canids: the bush dog, domestic dog (Canis familiaris, 2n = 78), grey wolf (C. lupus, 2n = 78), Chinese raccoon dog (Nyctereutes procyonoides procyonoides, 2n = 54+B), red fox (Vulpes vulpes, 2n = 34+B), and arctic fox (V. lagopus, 2n = 48-50) to specify the species position among Canidae. Using FISH with painting and BAC probes, we found that the distribution of canid evolutionarily conserved chromosome segments in the bush dog karyotype is similar to that of the domestic dog and grey wolf. The bush dog karyotype differs by 2 acrocentric chromosome pairs formed by tandem fusions of the canine (29;34) and (26;35) orthologues. An interstitial signal of the telomeric probe was observed in the (26;35) fusion site in the bush dog indicating a recent evolutionary origin of this rearrangement. Sequences and hybridisation patterns of satellite DNAs were compared, and a phylogenetic tree of the 6 canid species was constructed which confirmed the bush dog position close to the wolf-like canids, and apart from the raccoon dog and foxes. |
format |
Article in Journal/Newspaper |
author |
Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Fröhlich, Jan Vodicka, Roman Rubes, Jiri |
spellingShingle |
Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Fröhlich, Jan Vodicka, Roman Rubes, Jiri Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
author_facet |
Vozdova, Miluse Kubickova, Svatava Cernohorska, Halina Fröhlich, Jan Vodicka, Roman Rubes, Jiri |
author_sort |
Vozdova, Miluse |
title |
Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
title_short |
Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
title_full |
Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
title_fullStr |
Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
title_full_unstemmed |
Comparative Study of the Bush Dog (Speothos venaticus) Karyotype and Analysis of Satellite DNA Sequences and Their Chromosome Distribution in Six Species of Canidae |
title_sort |
comparative study of the bush dog (speothos venaticus) karyotype and analysis of satellite dna sequences and their chromosome distribution in six species of canidae |
publisher |
S. Karger AG |
publishDate |
2019 |
url |
http://dx.doi.org/10.1159/000503082 https://karger.com/cgr/article-pdf/159/2/88/3897439/000503082.pdf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Fox Arctic |
genre_facet |
Arctic Fox Arctic |
op_source |
Cytogenetic and Genome Research volume 159, issue 2, page 88-96 ISSN 1424-8581 1424-859X |
op_rights |
https://karger.com/pages/terms-and-conditions https://karger.com/pages/terms-and-conditions |
op_doi |
https://doi.org/10.1159/000503082 |
container_title |
Cytogenetic and Genome Research |
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159 |
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2 |
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88 |
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96 |
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1801371494444433408 |