Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous t...
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Online Access: | http://dx.doi.org/10.1159/000354882 https://www.karger.com/Article/Pdf/354882 |
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crskarger:10.1159/000354882 2024-10-13T14:10:46+00:00 Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences Symonová, R. Flajšhans, M. Sember, A. Havelka, M. Gela, D. Kořínková, T. Rodina, M. Rábová, M. Ráb, P. 2013 http://dx.doi.org/10.1159/000354882 https://www.karger.com/Article/Pdf/354882 en eng S. Karger AG https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses Cytogenetic and Genome Research volume 141, issue 2-3, page 153-162 ISSN 1424-8581 1424-859X journal-article 2013 crskarger https://doi.org/10.1159/000354882 2024-09-18T04:07:00Z We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid Siberian sturgeon A. baerii male (∼360 chromosomes), respectively. The ploidy levels of progenies were analyzed by karyotyping and flow cytometry. We found that the species-specific regions were surprisingly identifiable only on some micro- and small(er) macrochromosomes in hybrid metaphases. We hypothesize that these distinguishable regions are represented by species-specific repetitive sequences driven by more dynamic molecular evolutionary mechanisms. On larger chromosomes, GISH faintly visualized only blocks of pericentromeric and telomeric repetitive sequences, remaining regions were equally shared by both parental species. We concluded that the interspecies hybridization producing viable and even fertile progeny is enabled by the fact that genomes of the species involved are likely divergent at the level of the repetitive sequences only and probably highly conserved in the coding sequences. These small differences of coding sequences are in concordance with previous estimations of relatedness of examined species producing artificial as well as natural hybrids. CGH and GISH represent a challenge in sturgeon cytogenetics as a valuable though technically not simple tool to discriminate chromosomes of parental species in hybrids. The potentials and drawbacks of CGH and GISH application in sturgeons are discussed and further experimental possibilities are proposed. Article in Journal/Newspaper Siberian sturgeon Karger Cytogenetic and Genome Research 141 2-3 153 162 |
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Open Polar |
collection |
Karger |
op_collection_id |
crskarger |
language |
English |
description |
We applied comparative genomic hybridization (CGH) and genomic in situ hybridization (GISH) to examine genomes of artificially produced sturgeon hybrids between sterlet, Acipenser ruthenus female (∼120 chromosomes) or Russian sturgeon, A. gueldenstaedtii female (∼240 chromosomes) and a spontaneous triploid Siberian sturgeon A. baerii male (∼360 chromosomes), respectively. The ploidy levels of progenies were analyzed by karyotyping and flow cytometry. We found that the species-specific regions were surprisingly identifiable only on some micro- and small(er) macrochromosomes in hybrid metaphases. We hypothesize that these distinguishable regions are represented by species-specific repetitive sequences driven by more dynamic molecular evolutionary mechanisms. On larger chromosomes, GISH faintly visualized only blocks of pericentromeric and telomeric repetitive sequences, remaining regions were equally shared by both parental species. We concluded that the interspecies hybridization producing viable and even fertile progeny is enabled by the fact that genomes of the species involved are likely divergent at the level of the repetitive sequences only and probably highly conserved in the coding sequences. These small differences of coding sequences are in concordance with previous estimations of relatedness of examined species producing artificial as well as natural hybrids. CGH and GISH represent a challenge in sturgeon cytogenetics as a valuable though technically not simple tool to discriminate chromosomes of parental species in hybrids. The potentials and drawbacks of CGH and GISH application in sturgeons are discussed and further experimental possibilities are proposed. |
format |
Article in Journal/Newspaper |
author |
Symonová, R. Flajšhans, M. Sember, A. Havelka, M. Gela, D. Kořínková, T. Rodina, M. Rábová, M. Ráb, P. |
spellingShingle |
Symonová, R. Flajšhans, M. Sember, A. Havelka, M. Gela, D. Kořínková, T. Rodina, M. Rábová, M. Ráb, P. Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
author_facet |
Symonová, R. Flajšhans, M. Sember, A. Havelka, M. Gela, D. Kořínková, T. Rodina, M. Rábová, M. Ráb, P. |
author_sort |
Symonová, R. |
title |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_short |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_full |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_fullStr |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_full_unstemmed |
Molecular Cytogenetics in Artificial Hybrid and Highly Polyploid Sturgeons: An Evolutionary Story Narrated by Repetitive Sequences |
title_sort |
molecular cytogenetics in artificial hybrid and highly polyploid sturgeons: an evolutionary story narrated by repetitive sequences |
publisher |
S. Karger AG |
publishDate |
2013 |
url |
http://dx.doi.org/10.1159/000354882 https://www.karger.com/Article/Pdf/354882 |
genre |
Siberian sturgeon |
genre_facet |
Siberian sturgeon |
op_source |
Cytogenetic and Genome Research volume 141, issue 2-3, page 153-162 ISSN 1424-8581 1424-859X |
op_rights |
https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses |
op_doi |
https://doi.org/10.1159/000354882 |
container_title |
Cytogenetic and Genome Research |
container_volume |
141 |
container_issue |
2-3 |
container_start_page |
153 |
op_container_end_page |
162 |
_version_ |
1812818232003788800 |