Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis
A cytogenetic analysis was carried out using conventional staining, banding techniques and fluorescence in situ hybridization (FISH) in Italian populations of brown trout ( Salmo truttacomplex). All individuals analysed, belonging to the Atlantic (At), Marmoratus (Ma), Adriatic (Ad) and Mediterranea...
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Language: | English |
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S. Karger AG
2009
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Online Access: | http://dx.doi.org/10.1159/000200088 https://www.karger.com/Article/Pdf/200088 |
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crskarger:10.1159/000200088 2024-06-16T07:38:50+00:00 Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis Caputo, V. Giovannotti, M. Nisi Cerioni, P. Splendiani, A. Olmo, E. 2009 http://dx.doi.org/10.1159/000200088 https://www.karger.com/Article/Pdf/200088 en eng S. Karger AG https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses Cytogenetic and Genome Research volume 124, issue 1, page 51-62 ISSN 1424-8581 1424-859X journal-article 2009 crskarger https://doi.org/10.1159/000200088 2024-05-22T12:59:25Z A cytogenetic analysis was carried out using conventional staining, banding techniques and fluorescence in situ hybridization (FISH) in Italian populations of brown trout ( Salmo truttacomplex). All individuals analysed, belonging to the Atlantic (At), Marmoratus (Ma), Adriatic (Ad) and Mediterranean (Me) lineages, showed remarkable karyotype uniformity, with diploid complement of 2n = 80 chromosomes, arm number (NF) of 102 and invariable karyotype composition. Such uniformity was also observed with respect to the location of 5S rDNA and the active, i.e. silver-positive NOR sites. On the contrary, FISH with 28S ribosomal probe and fluorescent staining with CMA3 revealed that inactive NOR sites are more numerous in Ad and Me than in At and Ma lineages. A centromeric sequence was successfully isolated from Salmo trutta individuals by polymerase chain reaction (PCR)-based cloning, using primers designed from published Atlantic salmon (Salmo salar) satellite DNA sequences. This sequence had high AT content (65.3%) and short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellite repeats. The isolated satellite DNA clones were localized with FISH in the centromeric regions of the brown trout chromosomes, showing lineage-specific patterns. Because it is well known that AT-rich sequences can induce a pronounced DNA curvature, which in turn would promote faster and higher chromatin spiralization, it may be hypothesised that the wide distribution of this satellite in the S. trutta genome may have played a role in its karyotype stability. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within salmonids. Article in Journal/Newspaper Atlantic salmon Salmo salar Karger Cytogenetic and Genome Research 124 1 51 62 |
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English |
description |
A cytogenetic analysis was carried out using conventional staining, banding techniques and fluorescence in situ hybridization (FISH) in Italian populations of brown trout ( Salmo truttacomplex). All individuals analysed, belonging to the Atlantic (At), Marmoratus (Ma), Adriatic (Ad) and Mediterranean (Me) lineages, showed remarkable karyotype uniformity, with diploid complement of 2n = 80 chromosomes, arm number (NF) of 102 and invariable karyotype composition. Such uniformity was also observed with respect to the location of 5S rDNA and the active, i.e. silver-positive NOR sites. On the contrary, FISH with 28S ribosomal probe and fluorescent staining with CMA3 revealed that inactive NOR sites are more numerous in Ad and Me than in At and Ma lineages. A centromeric sequence was successfully isolated from Salmo trutta individuals by polymerase chain reaction (PCR)-based cloning, using primers designed from published Atlantic salmon (Salmo salar) satellite DNA sequences. This sequence had high AT content (65.3%) and short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellite repeats. The isolated satellite DNA clones were localized with FISH in the centromeric regions of the brown trout chromosomes, showing lineage-specific patterns. Because it is well known that AT-rich sequences can induce a pronounced DNA curvature, which in turn would promote faster and higher chromatin spiralization, it may be hypothesised that the wide distribution of this satellite in the S. trutta genome may have played a role in its karyotype stability. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within salmonids. |
format |
Article in Journal/Newspaper |
author |
Caputo, V. Giovannotti, M. Nisi Cerioni, P. Splendiani, A. Olmo, E. |
spellingShingle |
Caputo, V. Giovannotti, M. Nisi Cerioni, P. Splendiani, A. Olmo, E. Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
author_facet |
Caputo, V. Giovannotti, M. Nisi Cerioni, P. Splendiani, A. Olmo, E. |
author_sort |
Caputo, V. |
title |
Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
title_short |
Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
title_full |
Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
title_fullStr |
Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
title_full_unstemmed |
Chromosomal study of native and hatchery trouts from Italy ( Salmo trutta complex, Salmonidae): conventional and FISH analysis |
title_sort |
chromosomal study of native and hatchery trouts from italy ( salmo trutta complex, salmonidae): conventional and fish analysis |
publisher |
S. Karger AG |
publishDate |
2009 |
url |
http://dx.doi.org/10.1159/000200088 https://www.karger.com/Article/Pdf/200088 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Cytogenetic and Genome Research volume 124, issue 1, page 51-62 ISSN 1424-8581 1424-859X |
op_rights |
https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses |
op_doi |
https://doi.org/10.1159/000200088 |
container_title |
Cytogenetic and Genome Research |
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124 |
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1 |
container_start_page |
51 |
op_container_end_page |
62 |
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1802005562156646400 |