Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells
Background: Equine insect bite hypersensitivity (IBH) is an immediate-type hypersensitivity reaction provoked by insect-derived allergens. Icelandic horses living in Iceland do not have IBH due to absence of relevant insects, but acquire it at high frequency after being imported to mainland Europe....
Published in: | International Archives of Allergy and Immunology |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
S. Karger AG
2007
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1159/000106459 https://www.karger.com/Article/Pdf/106459 |
id |
crskarger:10.1159/000106459 |
---|---|
record_format |
openpolar |
spelling |
crskarger:10.1159/000106459 2024-06-23T07:53:57+00:00 Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells Hamza, E. Doherr, M.G. Bertoni, G. Jungi, T.W. Marti, E. 2007 http://dx.doi.org/10.1159/000106459 https://www.karger.com/Article/Pdf/106459 en eng S. Karger AG https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses International Archives of Allergy and Immunology volume 144, issue 4, page 325-337 ISSN 1018-2438 1423-0097 journal-article 2007 crskarger https://doi.org/10.1159/000106459 2024-06-12T04:07:24Z Background: Equine insect bite hypersensitivity (IBH) is an immediate-type hypersensitivity reaction provoked by insect-derived allergens. Icelandic horses living in Iceland do not have IBH due to absence of relevant insects, but acquire it at high frequency after being imported to mainland Europe. In contrast, their offspring born in mainland Europe has reduced IBH incidence. T helper 1 (Th1) and Th2 cells and cytokines were determined in Icelandic horses born in Iceland and on the continent and which either have IBH or are healthy. Methods: Peripheral blood mononuclear cells (PBMC) from these horses were stimulated for 18 h dur- ing summer and winter with polyclonal T cell stimuli, IBH allergen(s) or irrelevant allergen(s). Cells were analysed by flow cytometry for interferon-γ (IFN-γ) and interleukin-4 (IL-4); RNA was analysed for IFN-γ, IL-4, IL-5 and IL-13 mRNA. Results: During summer, but not during winter, IBH PBMC stimulated polyclonally showed reduced IFN-γ mRNA and IFN-γ-producing cells when compared with those of healthy horses, regardless of origin. PBMC stimulated polyclonally or with IBH allergen showed increased IL-4 mRNA levels and higher numbers of IL-4-producing cells when born in Iceland or showing IBH symptoms. IL-5 and IL-13 mRNA were modulated neither by disease nor by origin. Abrogation of IL-4 production in healthy horses born in mainland Europe may be due, at least in part, to IL-10. There was an increased level of IL-10 in supernatants from PBMC of healthy horses born in mainland Europe and stimulated polyclonally or with IBH allergen. Conclusions: Modulation of IBH incidence is governed by altered Th1/Th2 ratio, which might be influenced by IL-10. Article in Journal/Newspaper Iceland Karger International Archives of Allergy and Immunology 144 4 325 337 |
institution |
Open Polar |
collection |
Karger |
op_collection_id |
crskarger |
language |
English |
description |
Background: Equine insect bite hypersensitivity (IBH) is an immediate-type hypersensitivity reaction provoked by insect-derived allergens. Icelandic horses living in Iceland do not have IBH due to absence of relevant insects, but acquire it at high frequency after being imported to mainland Europe. In contrast, their offspring born in mainland Europe has reduced IBH incidence. T helper 1 (Th1) and Th2 cells and cytokines were determined in Icelandic horses born in Iceland and on the continent and which either have IBH or are healthy. Methods: Peripheral blood mononuclear cells (PBMC) from these horses were stimulated for 18 h dur- ing summer and winter with polyclonal T cell stimuli, IBH allergen(s) or irrelevant allergen(s). Cells were analysed by flow cytometry for interferon-γ (IFN-γ) and interleukin-4 (IL-4); RNA was analysed for IFN-γ, IL-4, IL-5 and IL-13 mRNA. Results: During summer, but not during winter, IBH PBMC stimulated polyclonally showed reduced IFN-γ mRNA and IFN-γ-producing cells when compared with those of healthy horses, regardless of origin. PBMC stimulated polyclonally or with IBH allergen showed increased IL-4 mRNA levels and higher numbers of IL-4-producing cells when born in Iceland or showing IBH symptoms. IL-5 and IL-13 mRNA were modulated neither by disease nor by origin. Abrogation of IL-4 production in healthy horses born in mainland Europe may be due, at least in part, to IL-10. There was an increased level of IL-10 in supernatants from PBMC of healthy horses born in mainland Europe and stimulated polyclonally or with IBH allergen. Conclusions: Modulation of IBH incidence is governed by altered Th1/Th2 ratio, which might be influenced by IL-10. |
format |
Article in Journal/Newspaper |
author |
Hamza, E. Doherr, M.G. Bertoni, G. Jungi, T.W. Marti, E. |
spellingShingle |
Hamza, E. Doherr, M.G. Bertoni, G. Jungi, T.W. Marti, E. Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
author_facet |
Hamza, E. Doherr, M.G. Bertoni, G. Jungi, T.W. Marti, E. |
author_sort |
Hamza, E. |
title |
Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
title_short |
Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
title_full |
Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
title_fullStr |
Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
title_full_unstemmed |
Modulation of Allergy Incidence in Icelandic Horses Is Associated with a Change in IL-4-Producing T Cells |
title_sort |
modulation of allergy incidence in icelandic horses is associated with a change in il-4-producing t cells |
publisher |
S. Karger AG |
publishDate |
2007 |
url |
http://dx.doi.org/10.1159/000106459 https://www.karger.com/Article/Pdf/106459 |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
International Archives of Allergy and Immunology volume 144, issue 4, page 325-337 ISSN 1018-2438 1423-0097 |
op_rights |
https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses |
op_doi |
https://doi.org/10.1159/000106459 |
container_title |
International Archives of Allergy and Immunology |
container_volume |
144 |
container_issue |
4 |
container_start_page |
325 |
op_container_end_page |
337 |
_version_ |
1802645867979603968 |