Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua)
The aim of the present study was to explore the electrophysiological properties of pituitary cells from Atlantic cod (Gadus morhua), as a basis for future studies of the signaling pathways involved in the control of pituitary secretion in this species. Primary cultures of pituitary cells from maturi...
Published in: | Neuroendocrinology |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
S. Karger AG
2007
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1159/000103867 https://www.karger.com/Article/Pdf/103867 |
id |
crskarger:10.1159/000103867 |
---|---|
record_format |
openpolar |
spelling |
crskarger:10.1159/000103867 2024-06-16T07:38:33+00:00 Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) Haug, Trude M. Hodne, Kjetil Weltzien, Finn-Arne Sand, Olav 2007 http://dx.doi.org/10.1159/000103867 https://www.karger.com/Article/Pdf/103867 en eng S. Karger AG https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses Neuroendocrinology volume 86, issue 1, page 38-47 ISSN 0028-3835 1423-0194 journal-article 2007 crskarger https://doi.org/10.1159/000103867 2024-05-22T13:00:08Z The aim of the present study was to explore the electrophysiological properties of pituitary cells from Atlantic cod (Gadus morhua), as a basis for future studies of the signaling pathways involved in the control of pituitary secretion in this species. Primary cultures of pituitary cells from maturing Atlantic cod were prepared by trypsin treatment and mechanical dispersion. Electrophysiological recordings were performed using the perforated patch clamp method. A subpopulation of large cells were selected for recordings. Spontaneous action potentials were observed in about 30% of the cells. The action potentials displayed a fast initial spike followed by a prolonged plateau. Correspondingly, the inward current elicited by depolarizing steps consisted of both a transient, tetrodotoxin-sensitive Na + component and a nifedipine-sensitive Ca 2+ component that was sustained when Ba 2+ replaced Ca 2+ as current carrier. The outward current was partially blocked both by 5 m M tetraethylammonium and 10 m M 4-aminopyridine. The voltage-activated ion channels present in these cells largely correspond to the ion channels of pituitary cells in other teleosts (goldfish, Carassius auratus, and tilapia, Oreochromis mossambicus) and mammals, although differences exist regarding the shape and duration of action potentials. Article in Journal/Newspaper atlantic cod Gadus morhua Karger Neuroendocrinology 86 1 38 47 |
institution |
Open Polar |
collection |
Karger |
op_collection_id |
crskarger |
language |
English |
description |
The aim of the present study was to explore the electrophysiological properties of pituitary cells from Atlantic cod (Gadus morhua), as a basis for future studies of the signaling pathways involved in the control of pituitary secretion in this species. Primary cultures of pituitary cells from maturing Atlantic cod were prepared by trypsin treatment and mechanical dispersion. Electrophysiological recordings were performed using the perforated patch clamp method. A subpopulation of large cells were selected for recordings. Spontaneous action potentials were observed in about 30% of the cells. The action potentials displayed a fast initial spike followed by a prolonged plateau. Correspondingly, the inward current elicited by depolarizing steps consisted of both a transient, tetrodotoxin-sensitive Na + component and a nifedipine-sensitive Ca 2+ component that was sustained when Ba 2+ replaced Ca 2+ as current carrier. The outward current was partially blocked both by 5 m M tetraethylammonium and 10 m M 4-aminopyridine. The voltage-activated ion channels present in these cells largely correspond to the ion channels of pituitary cells in other teleosts (goldfish, Carassius auratus, and tilapia, Oreochromis mossambicus) and mammals, although differences exist regarding the shape and duration of action potentials. |
format |
Article in Journal/Newspaper |
author |
Haug, Trude M. Hodne, Kjetil Weltzien, Finn-Arne Sand, Olav |
spellingShingle |
Haug, Trude M. Hodne, Kjetil Weltzien, Finn-Arne Sand, Olav Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
author_facet |
Haug, Trude M. Hodne, Kjetil Weltzien, Finn-Arne Sand, Olav |
author_sort |
Haug, Trude M. |
title |
Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
title_short |
Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
title_full |
Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
title_fullStr |
Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
title_full_unstemmed |
Electrophysiological Properties of Pituitary Cells in Primary Culture from Atlantic Cod (Gadus morhua) |
title_sort |
electrophysiological properties of pituitary cells in primary culture from atlantic cod (gadus morhua) |
publisher |
S. Karger AG |
publishDate |
2007 |
url |
http://dx.doi.org/10.1159/000103867 https://www.karger.com/Article/Pdf/103867 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Neuroendocrinology volume 86, issue 1, page 38-47 ISSN 0028-3835 1423-0194 |
op_rights |
https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses |
op_doi |
https://doi.org/10.1159/000103867 |
container_title |
Neuroendocrinology |
container_volume |
86 |
container_issue |
1 |
container_start_page |
38 |
op_container_end_page |
47 |
_version_ |
1802005228619300864 |