Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla)
To investigate the ability of hypothalamic and peripheral factors to directly regulate growth hormone (GH) release in a primitive teleost, the European eel ( Anguilla anguilla L.), we used primary cultures of dispersed pituitary cells. When cultured for 12 days in a serum-free medium, pituitary cell...
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Online Access: | http://dx.doi.org/10.1159/000054327 https://www.karger.com/Article/Pdf/54327 |
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crskarger:10.1159/000054327 2024-06-16T07:33:27+00:00 Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) Rousseau, Karine Huang, Yung-Sen Le Belle, Nadine Vidal, Bernadette Marchelidon, Jacques Epelbaum, Jacques Dufour, Sylvie 1998 http://dx.doi.org/10.1159/000054327 https://www.karger.com/Article/Pdf/54327 en eng S. Karger AG https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses Neuroendocrinology volume 67, issue 4, page 301-309 ISSN 0028-3835 1423-0194 journal-article 1998 crskarger https://doi.org/10.1159/000054327 2024-05-22T12:59:21Z To investigate the ability of hypothalamic and peripheral factors to directly regulate growth hormone (GH) release in a primitive teleost, the European eel ( Anguilla anguilla L.), we used primary cultures of dispersed pituitary cells. When cultured for 12 days in a serum-free medium, pituitary cells continuously released large amounts of GH, which exceeded the initial cellular content. Somatotropin-release inhibiting hormone (SRIH-14) dose-dependently inhibited GH release (EC 50 0.75 n M) up to a maximal inhibitory effect of 95%. No desensitization of somatotropes to SRIH was observed over the 12 days of culture. Use of receptor subtype-selective SRIH agonists suggests the existence on eel somatotropes of SRIH receptor(s) related to the mammalian sst 2 /sst 3 /sst 5 class rather than to the sst 1 /sst 4 class. Insulin-like growth factor 1 (IGF1) dose-dependently inhibited GH release (EC 50 0.03 n M) up to a maximal inhibitory effect of 85%, without desensitization. IGF1 and IGF2 were equipotent in inhibiting GH release, whereas insulin was 1,000 times less active, suggesting the implication of a receptor related to the mammalian IGF type 1 receptor. These results indicate that eel somatotropes are active in vitro without any specific additional factors, and suggest the existence of a dominant inhibitory control of GH release in vivo. Two potential candidates for this chronic negative regulation are a neurohormone, SRIH and a circulating factor, IGF1. These data underline the early evolutionary origin of the molecular and functional SRIH-GH-IGF1 neuroendocrine axis in vertebrates. Article in Journal/Newspaper Anguilla anguilla Karger Neuroendocrinology 67 4 301 309 |
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language |
English |
description |
To investigate the ability of hypothalamic and peripheral factors to directly regulate growth hormone (GH) release in a primitive teleost, the European eel ( Anguilla anguilla L.), we used primary cultures of dispersed pituitary cells. When cultured for 12 days in a serum-free medium, pituitary cells continuously released large amounts of GH, which exceeded the initial cellular content. Somatotropin-release inhibiting hormone (SRIH-14) dose-dependently inhibited GH release (EC 50 0.75 n M) up to a maximal inhibitory effect of 95%. No desensitization of somatotropes to SRIH was observed over the 12 days of culture. Use of receptor subtype-selective SRIH agonists suggests the existence on eel somatotropes of SRIH receptor(s) related to the mammalian sst 2 /sst 3 /sst 5 class rather than to the sst 1 /sst 4 class. Insulin-like growth factor 1 (IGF1) dose-dependently inhibited GH release (EC 50 0.03 n M) up to a maximal inhibitory effect of 85%, without desensitization. IGF1 and IGF2 were equipotent in inhibiting GH release, whereas insulin was 1,000 times less active, suggesting the implication of a receptor related to the mammalian IGF type 1 receptor. These results indicate that eel somatotropes are active in vitro without any specific additional factors, and suggest the existence of a dominant inhibitory control of GH release in vivo. Two potential candidates for this chronic negative regulation are a neurohormone, SRIH and a circulating factor, IGF1. These data underline the early evolutionary origin of the molecular and functional SRIH-GH-IGF1 neuroendocrine axis in vertebrates. |
format |
Article in Journal/Newspaper |
author |
Rousseau, Karine Huang, Yung-Sen Le Belle, Nadine Vidal, Bernadette Marchelidon, Jacques Epelbaum, Jacques Dufour, Sylvie |
spellingShingle |
Rousseau, Karine Huang, Yung-Sen Le Belle, Nadine Vidal, Bernadette Marchelidon, Jacques Epelbaum, Jacques Dufour, Sylvie Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
author_facet |
Rousseau, Karine Huang, Yung-Sen Le Belle, Nadine Vidal, Bernadette Marchelidon, Jacques Epelbaum, Jacques Dufour, Sylvie |
author_sort |
Rousseau, Karine |
title |
Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
title_short |
Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
title_full |
Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
title_fullStr |
Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
title_full_unstemmed |
Long-Term Inhibitory Effects of Somatostatin and Insulin-Like Growth Factor 1 on Growth Hormone Release by Serum-Free Primary Culture of Pituitary Cells from European Eel (Anguilla anguilla) |
title_sort |
long-term inhibitory effects of somatostatin and insulin-like growth factor 1 on growth hormone release by serum-free primary culture of pituitary cells from european eel (anguilla anguilla) |
publisher |
S. Karger AG |
publishDate |
1998 |
url |
http://dx.doi.org/10.1159/000054327 https://www.karger.com/Article/Pdf/54327 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Neuroendocrinology volume 67, issue 4, page 301-309 ISSN 0028-3835 1423-0194 |
op_rights |
https://www.karger.com/Services/SiteLicenses https://www.karger.com/Services/SiteLicenses |
op_doi |
https://doi.org/10.1159/000054327 |
container_title |
Neuroendocrinology |
container_volume |
67 |
container_issue |
4 |
container_start_page |
301 |
op_container_end_page |
309 |
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1802004517350277120 |