Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease
Gills from Atlantic salmon with experimentally induced amoebic gill disease ( Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invagination...
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crsagepubl:10.1354/vp.44-5-663 2024-10-06T13:47:20+00:00 Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease Lovy, J. Becker, J. A. Speare, D. J. Wadowska, D. W. Wright, G. M. Powell, M. D. 2007 http://dx.doi.org/10.1354/vp.44-5-663 https://journals.sagepub.com/doi/pdf/10.1354/vp.44-5-663 https://journals.sagepub.com/doi/full-xml/10.1354/vp.44-5-663 en eng SAGE Publications https://journals.sagepub.com/page/policies/text-and-data-mining-license Veterinary Pathology volume 44, issue 5, page 663-671 ISSN 0300-9858 1544-2217 journal-article 2007 crsagepubl https://doi.org/10.1354/vp.44-5-663 2024-09-10T04:27:46Z Gills from Atlantic salmon with experimentally induced amoebic gill disease ( Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations of epithelial cells. The amoebae had various densities along the plasma membrane and contained electron-dense deposits within their cytoplasm. Surface epithelial cells sloughed from the gills and had features consistent with apoptosis, including rounded shape, loss of surface microridges, and hypercondensation of nuclear chromatin. Affected areas of gills had fusion of secondary lamellae with interlamellar spaces occupied by mitotic epithelial cells and eosinophils. Eosinophils contained abundant fusiform-shaped granules that measured approximately 1 μm long and 360 nm wide. The granule consisted of an electron-dense matrix with a central inclusion that was less electron-dense, consisting of particulate and fibrillar material. In many instances, the central inclusion appeared empty and 90% of the eosinophils had morphology suggestive of piecemeal degranulation. Also observed within affected areas were a few neutrophils, mucous cells releasing mucus, and a small number of dendritic-like cells. Article in Journal/Newspaper Atlantic salmon Salmo salar SAGE Publications Veterinary Pathology 44 5 663 671 |
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English |
description |
Gills from Atlantic salmon with experimentally induced amoebic gill disease ( Neoparamoeba spp.) were examined with transmission electron microscopy to assess pathology and host-cell responses. Amoebae were found either on the surface epithelium or with pseudopodia extending deeply into invaginations of epithelial cells. The amoebae had various densities along the plasma membrane and contained electron-dense deposits within their cytoplasm. Surface epithelial cells sloughed from the gills and had features consistent with apoptosis, including rounded shape, loss of surface microridges, and hypercondensation of nuclear chromatin. Affected areas of gills had fusion of secondary lamellae with interlamellar spaces occupied by mitotic epithelial cells and eosinophils. Eosinophils contained abundant fusiform-shaped granules that measured approximately 1 μm long and 360 nm wide. The granule consisted of an electron-dense matrix with a central inclusion that was less electron-dense, consisting of particulate and fibrillar material. In many instances, the central inclusion appeared empty and 90% of the eosinophils had morphology suggestive of piecemeal degranulation. Also observed within affected areas were a few neutrophils, mucous cells releasing mucus, and a small number of dendritic-like cells. |
format |
Article in Journal/Newspaper |
author |
Lovy, J. Becker, J. A. Speare, D. J. Wadowska, D. W. Wright, G. M. Powell, M. D. |
spellingShingle |
Lovy, J. Becker, J. A. Speare, D. J. Wadowska, D. W. Wright, G. M. Powell, M. D. Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
author_facet |
Lovy, J. Becker, J. A. Speare, D. J. Wadowska, D. W. Wright, G. M. Powell, M. D. |
author_sort |
Lovy, J. |
title |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
title_short |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
title_full |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
title_fullStr |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
title_full_unstemmed |
Ultrastructural Examination of the Host Cellular Response in the Gills of Atlantic Salmon, Salmo salar, with Amoebic Gill Disease |
title_sort |
ultrastructural examination of the host cellular response in the gills of atlantic salmon, salmo salar, with amoebic gill disease |
publisher |
SAGE Publications |
publishDate |
2007 |
url |
http://dx.doi.org/10.1354/vp.44-5-663 https://journals.sagepub.com/doi/pdf/10.1354/vp.44-5-663 https://journals.sagepub.com/doi/full-xml/10.1354/vp.44-5-663 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Veterinary Pathology volume 44, issue 5, page 663-671 ISSN 0300-9858 1544-2217 |
op_rights |
https://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1354/vp.44-5-663 |
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Veterinary Pathology |
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44 |
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5 |
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663 |
op_container_end_page |
671 |
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1812175560039727104 |