Secondary Metabolites from a Strain of Alternaria tenuissima Isolated from Northern Manitoba Soil

• Published in: Natural Product Communications
• Main Authors: Anyanwu, Chukwudi S., Sorensen, John L.
• Format: Article in Journal/Newspaper
• Language: English
• Published: SAGE Publications 2015
• Subjects: Complementary and alternative medicine; Plant Science; Drug Discovery; Pharmacology; General Medicine; Wapusk national park
• Online Access: http://dx.doi.org/10.1177/1934578x1501000112; http://journals.sagepub.com/doi/pdf/10.1177/1934578X1501000112

Deoxyphomalone (1), dimethyl 4-methyl-2,6-pyridinedicarboxylate (2), stemphyperylenol (3), and N-methyl-2-pyrrolidone (4) were isolated from the fermentation broth of a strain of the fungus, Alternaria tenuissima. This fungus was isolated from the soil underlying the lichen, Peltigera didactyla, which had been collected from Wapusk National Park in Northern Manitoba. The structures of the compounds were determined by comprehensive analysis of their spectroscopic data including FT-IR, 1D and 2D NMR spectroscopy and mass spectrometry; and their bioactivities were tested against E.coli cells. The taxonomic identity of the fungus was confirmed by ITS sequencing of its ribosomal DNA.