Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B)
Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betuli...
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crsagepubl:10.1177/1534735418757912 2024-09-30T14:38:03+00:00 Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) Géry, Antoine Dubreule, Christelle André, Véronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David 2018 http://dx.doi.org/10.1177/1534735418757912 http://journals.sagepub.com/doi/pdf/10.1177/1534735418757912 http://journals.sagepub.com/doi/full-xml/10.1177/1534735418757912 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Integrative Cancer Therapies volume 17, issue 3, page 832-843 ISSN 1534-7354 1552-695X journal-article 2018 crsagepubl https://doi.org/10.1177/1534735418757912 2024-09-17T04:41:13Z Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells. Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line). Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure). Article in Journal/Newspaper khanty SAGE Publications Canada Integrative Cancer Therapies 17 3 832 843 |
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Background: Inonotus obliquus, also known as Chaga, is a parasitic fungus growing on birches and used in traditional medicine (especially by Khanty people) to treat various health problems. In this study, we aimed to quantify the 3 metabolites frequently cited in literature, that is, betulin, betulinic acid, and inotodiol in the Chaga recently discovered in forests located in Normandy (France), and to compare their concentrations with Ukrainian and Canadian Chaga. This study also explores the cytotoxicity of the French Chaga against cancer-derived cells and transformed cells. Methods: A quantification method by HPLC-MS-MS (high-performance liquid chromatography–tandem mass spectrometry) of betulin, betulinic acid, and inotodiol was developed to study the French Chaga and compare the concentration of these metabolites with extracts provided from Chaga growing in Canada and Ukraine. This method was also used to identify and quantify those 3 compounds in other traditional preparations of Chaga (aqueous extract, infusion, and decoction). Among these preparations, the aqueous extract that contains betulin, betulinic acid, and inotodiol was chosen to evaluate and compare its cytotoxic activity toward human lung adenocarcinoma cells (A549 line) and human bronchial epithelial cells (BEAS-2B line). Results: French Chaga contains betulin and betulinic acid at higher levels than in other Chaga, whereas the concentration of inotodiol is greater in the Canadian Chaga. Moreover, the results highlighted a cytotoxic activity of the Chaga’s aqueous extract after 48 and 72 hours of exposure with a higher effect on cancer-derived cells A549 than on normal transformed cells BEAS-2B ( P = 0.025 after 48 hours of exposure and P = 0.004 after 72 hours of exposure). |
format |
Article in Journal/Newspaper |
author |
Géry, Antoine Dubreule, Christelle André, Véronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David |
spellingShingle |
Géry, Antoine Dubreule, Christelle André, Véronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
author_facet |
Géry, Antoine Dubreule, Christelle André, Véronique Rioult, Jean-Philippe Bouchart, Valérie Heutte, Natacha Eldin de Pécoulas, Philippe Krivomaz, Tetyana Garon, David |
author_sort |
Géry, Antoine |
title |
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_short |
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_full |
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_fullStr |
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_full_unstemmed |
Chaga ( Inonotus obliquus), a Future Potential Medicinal Fungus in Oncology? A Chemical Study and a Comparison of the Cytotoxicity Against Human Lung Adenocarcinoma Cells (A549) and Human Bronchial Epithelial Cells (BEAS-2B) |
title_sort |
chaga ( inonotus obliquus), a future potential medicinal fungus in oncology? a chemical study and a comparison of the cytotoxicity against human lung adenocarcinoma cells (a549) and human bronchial epithelial cells (beas-2b) |
publisher |
SAGE Publications |
publishDate |
2018 |
url |
http://dx.doi.org/10.1177/1534735418757912 http://journals.sagepub.com/doi/pdf/10.1177/1534735418757912 http://journals.sagepub.com/doi/full-xml/10.1177/1534735418757912 |
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Canada |
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Canada |
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khanty |
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khanty |
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Integrative Cancer Therapies volume 17, issue 3, page 832-843 ISSN 1534-7354 1552-695X |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/1534735418757912 |
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Integrative Cancer Therapies |
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17 |
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3 |
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832 |
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843 |
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