Secondary Palate Development in the Dog ( Canis lupus familiaris )
Objective: To investigate the gestational timing of morphologic events in normal canine secondary palate development as a baseline for studies in dog models of isolated cleft palate (CP). Methods: Beagle and beagle/cocker spaniel-hybrid fetal dogs were obtained by cesarean-section on various days of...
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Online Access: | http://dx.doi.org/10.1177/1055665620943771 http://journals.sagepub.com/doi/pdf/10.1177/1055665620943771 http://journals.sagepub.com/doi/full-xml/10.1177/1055665620943771 |
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crsagepubl:10.1177/1055665620943771 2024-05-12T08:02:14+00:00 Secondary Palate Development in the Dog ( Canis lupus familiaris ) Freiberger, Katharina Hemker, Shelby McAnally, Ryan King, Rachel Meyers-Wallen, Vicki N. Schutte, Brian C. Fyfe, John C. National Institute of Child Health and Human Development The Herndon Canine Disease Research Fund, Baker Institute for Animal Health, Cornell University, Ithaca, NY 2020 http://dx.doi.org/10.1177/1055665620943771 http://journals.sagepub.com/doi/pdf/10.1177/1055665620943771 http://journals.sagepub.com/doi/full-xml/10.1177/1055665620943771 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license The Cleft Palate-Craniofacial Journal volume 58, issue 2, page 230-236 ISSN 1055-6656 1545-1569 Otorhinolaryngology Oral Surgery journal-article 2020 crsagepubl https://doi.org/10.1177/1055665620943771 2024-04-18T08:32:50Z Objective: To investigate the gestational timing of morphologic events in normal canine secondary palate development as a baseline for studies in dog models of isolated cleft palate (CP). Methods: Beagle and beagle/cocker spaniel-hybrid fetal dogs were obtained by cesarean-section on various days of gestation, timed from the initial rise of serum progesterone concentration. Morphology of fetal heads was determined by examining serial coronal sections. Results: On gestational day 35 (d35), the palatal shelves pointed ventrally alongside the tongue. On d36, palatal shelves were elongated and elevated to a horizontal position above the tongue but were not touching. On d37, palatine shelves and vomer were touching, but the medial epithelial seam (MES) between the apposed shelves remained. Immunostaining with epithelial protein markers showed that the MES gradually dissolved and was replaced by mesenchyme during d37-d44, and palate fusion was complete by d44. Examination of remnant MES suggested that fusion of palatal shelves began in mid-palate and moved rostrally and caudally. Conclusion: Palate development occurs in dogs in the steps described in humans and mice, but palate closure occurs at an intermediate time in gestation. These normative data will form the basis of future studies to determine pathophysiologic mechanisms in dog models of CP. Added clinical significance is the enhancement of dogs as a large animal model to test new approaches for palate repair, with the obvious advantage of achieving full maturity within 2 years rather than 2 decades. Article in Journal/Newspaper Canis lupus SAGE Publications The Cleft Palate-Craniofacial Journal 58 2 230 236 |
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SAGE Publications |
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language |
English |
topic |
Otorhinolaryngology Oral Surgery |
spellingShingle |
Otorhinolaryngology Oral Surgery Freiberger, Katharina Hemker, Shelby McAnally, Ryan King, Rachel Meyers-Wallen, Vicki N. Schutte, Brian C. Fyfe, John C. Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
topic_facet |
Otorhinolaryngology Oral Surgery |
description |
Objective: To investigate the gestational timing of morphologic events in normal canine secondary palate development as a baseline for studies in dog models of isolated cleft palate (CP). Methods: Beagle and beagle/cocker spaniel-hybrid fetal dogs were obtained by cesarean-section on various days of gestation, timed from the initial rise of serum progesterone concentration. Morphology of fetal heads was determined by examining serial coronal sections. Results: On gestational day 35 (d35), the palatal shelves pointed ventrally alongside the tongue. On d36, palatal shelves were elongated and elevated to a horizontal position above the tongue but were not touching. On d37, palatine shelves and vomer were touching, but the medial epithelial seam (MES) between the apposed shelves remained. Immunostaining with epithelial protein markers showed that the MES gradually dissolved and was replaced by mesenchyme during d37-d44, and palate fusion was complete by d44. Examination of remnant MES suggested that fusion of palatal shelves began in mid-palate and moved rostrally and caudally. Conclusion: Palate development occurs in dogs in the steps described in humans and mice, but palate closure occurs at an intermediate time in gestation. These normative data will form the basis of future studies to determine pathophysiologic mechanisms in dog models of CP. Added clinical significance is the enhancement of dogs as a large animal model to test new approaches for palate repair, with the obvious advantage of achieving full maturity within 2 years rather than 2 decades. |
author2 |
National Institute of Child Health and Human Development The Herndon Canine Disease Research Fund, Baker Institute for Animal Health, Cornell University, Ithaca, NY |
format |
Article in Journal/Newspaper |
author |
Freiberger, Katharina Hemker, Shelby McAnally, Ryan King, Rachel Meyers-Wallen, Vicki N. Schutte, Brian C. Fyfe, John C. |
author_facet |
Freiberger, Katharina Hemker, Shelby McAnally, Ryan King, Rachel Meyers-Wallen, Vicki N. Schutte, Brian C. Fyfe, John C. |
author_sort |
Freiberger, Katharina |
title |
Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
title_short |
Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
title_full |
Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
title_fullStr |
Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
title_full_unstemmed |
Secondary Palate Development in the Dog ( Canis lupus familiaris ) |
title_sort |
secondary palate development in the dog ( canis lupus familiaris ) |
publisher |
SAGE Publications |
publishDate |
2020 |
url |
http://dx.doi.org/10.1177/1055665620943771 http://journals.sagepub.com/doi/pdf/10.1177/1055665620943771 http://journals.sagepub.com/doi/full-xml/10.1177/1055665620943771 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
The Cleft Palate-Craniofacial Journal volume 58, issue 2, page 230-236 ISSN 1055-6656 1545-1569 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/1055665620943771 |
container_title |
The Cleft Palate-Craniofacial Journal |
container_volume |
58 |
container_issue |
2 |
container_start_page |
230 |
op_container_end_page |
236 |
_version_ |
1798844325484822528 |