Immunohistochemical Identification of Infectious Pancreatic Necrosis Virus in Paraffin-Embedded Tissues of Atlantic Salmon ( Salmo Salar)

• Published in: Journal of Veterinary Diagnostic Investigation
• Main Authors: Evensen, Øystein, Rimstad, Espen
• Format: Article in Journal/Newspaper
• Language: English
• Published: SAGE Publications 1990
• Subjects: Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1177/104063879000200406; http://journals.sagepub.com/doi/pdf/10.1177/104063879000200406

Infectious pancreatic necrosis virus serotype Sp was identified by immunohistochemistry in formaldehyde-fixed and paraffin-embedded tissue of Atlantic salmon ( Salmo salar). The immunoreaction was present in degenerating and necrotic cells in exocrine pancreatic cells. Cross reactions were observed with rabbit antisera against serotypes Sp, Ab, and VR-299 in neutralization tests and western blotting. Immunohistochemically, only Sp antiserum produced positive immunostaining to Sp antigens, whereas antisera to serotypes Ab and VR-299 were negative.