Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon
Desmozoon lepeophtherii is a microsporidian associated with gill disease in farmed Atlantic salmon ( Salmo salar). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individ...
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crsagepubl:10.1177/1040638719887707 2023-05-15T15:29:17+02:00 Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon Herrero, Ana Padrós, Francesc Pflaum, Sara Matthews, Chris del-Pozo, Jorge Rodger, Hamish D. Dagleish, Mark P. Thompson, Kim D. 2019 http://dx.doi.org/10.1177/1040638719887707 http://journals.sagepub.com/doi/pdf/10.1177/1040638719887707 http://journals.sagepub.com/doi/full-xml/10.1177/1040638719887707 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 32, issue 1, page 142-146 ISSN 1040-6387 1943-4936 General Veterinary journal-article 2019 crsagepubl https://doi.org/10.1177/1040638719887707 2022-08-12T11:31:33Z Desmozoon lepeophtherii is a microsporidian associated with gill disease in farmed Atlantic salmon ( Salmo salar). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect D. lepeophtherii spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eosin (H&E), Gram–Twort (GT), calcofluor white (CW), and immunohistochemistry (IHC). Using CW as a benchmark to calculate a relative ratio, IHC consistently detected more spores than CW (median: 1.3), followed by GT (median: 0.2) and H&E (median: 0.1). IHC detected significantly more spores than GT ( p < 0.05) and H&E ( p < 0.05), and GT more than H&E ( p < 0.05). We found significant underestimation of numbers of microsporidia spores in gill disease in Atlantic salmon using conventional histochemical stains and recommend the use of CW or IHC to detect the parasite in tissue sections. Article in Journal/Newspaper Atlantic salmon Salmo salar SAGE Publications (via Crossref) Journal of Veterinary Diagnostic Investigation 32 1 142 146 |
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General Veterinary |
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General Veterinary Herrero, Ana Padrós, Francesc Pflaum, Sara Matthews, Chris del-Pozo, Jorge Rodger, Hamish D. Dagleish, Mark P. Thompson, Kim D. Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
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General Veterinary |
description |
Desmozoon lepeophtherii is a microsporidian associated with gill disease in farmed Atlantic salmon ( Salmo salar). Detection of the parasite in histologic tissue sections is challenging using common histochemical stains given that the small, widely distributed parasite spores typically occur individually or in small clusters. We compared the ability of 4 histologic methods to detect D. lepeophtherii spores in serial sections of Atlantic salmon gill tissue: hematoxylin and eosin (H&E), Gram–Twort (GT), calcofluor white (CW), and immunohistochemistry (IHC). Using CW as a benchmark to calculate a relative ratio, IHC consistently detected more spores than CW (median: 1.3), followed by GT (median: 0.2) and H&E (median: 0.1). IHC detected significantly more spores than GT ( p < 0.05) and H&E ( p < 0.05), and GT more than H&E ( p < 0.05). We found significant underestimation of numbers of microsporidia spores in gill disease in Atlantic salmon using conventional histochemical stains and recommend the use of CW or IHC to detect the parasite in tissue sections. |
format |
Article in Journal/Newspaper |
author |
Herrero, Ana Padrós, Francesc Pflaum, Sara Matthews, Chris del-Pozo, Jorge Rodger, Hamish D. Dagleish, Mark P. Thompson, Kim D. |
author_facet |
Herrero, Ana Padrós, Francesc Pflaum, Sara Matthews, Chris del-Pozo, Jorge Rodger, Hamish D. Dagleish, Mark P. Thompson, Kim D. |
author_sort |
Herrero, Ana |
title |
Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
title_short |
Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
title_full |
Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
title_fullStr |
Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
title_full_unstemmed |
Comparison of histologic methods for the detection of Desmozoon lepeophtherii spores in the gills of Atlantic salmon |
title_sort |
comparison of histologic methods for the detection of desmozoon lepeophtherii spores in the gills of atlantic salmon |
publisher |
SAGE Publications |
publishDate |
2019 |
url |
http://dx.doi.org/10.1177/1040638719887707 http://journals.sagepub.com/doi/pdf/10.1177/1040638719887707 http://journals.sagepub.com/doi/full-xml/10.1177/1040638719887707 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Veterinary Diagnostic Investigation volume 32, issue 1, page 142-146 ISSN 1040-6387 1943-4936 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/1040638719887707 |
container_title |
Journal of Veterinary Diagnostic Investigation |
container_volume |
32 |
container_issue |
1 |
container_start_page |
142 |
op_container_end_page |
146 |
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1766359669882748928 |