Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos)
A 33-year-old brown bear ( Ursus arctos) was evaluated for chronic cough, partial anorexia, and lethargy in early fall of 2009. Radiographs revealed a generalized increase in interstitial density with focal lung field consolidation and air bronchograms more prevalent in the cranial lung lobes. Trach...
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crsagepubl:10.1177/1040638711406976 2024-10-06T13:53:18+00:00 Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) Highland, Margaret A. Chaturvedi, Sudha Perez, Michael Steinberg, Howard Wallace, Roberta 2011 http://dx.doi.org/10.1177/1040638711406976 https://journals.sagepub.com/doi/pdf/10.1177/1040638711406976 https://journals.sagepub.com/doi/full-xml/10.1177/1040638711406976 en eng SAGE Publications https://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 23, issue 4, page 764-769 ISSN 1040-6387 1943-4936 journal-article 2011 crsagepubl https://doi.org/10.1177/1040638711406976 2024-09-10T04:23:42Z A 33-year-old brown bear ( Ursus arctos) was evaluated for chronic cough, partial anorexia, and lethargy in early fall of 2009. Radiographs revealed a generalized increase in interstitial density with focal lung field consolidation and air bronchograms more prevalent in the cranial lung lobes. Tracheal sputum and wash fluid grew mixed bacteria and 2 species of Candida on bacterial and fungal cultures, respectively. Serum was negative for antibodies to Aspergillus, Blastomyces, Coccidioides, and Histoplasma by semiquantitative radial immunodiffusion. Antimicrobial and antifungal treatment was administered. The bear died 1 month after entering hibernation. Gross necropsy revealed coalescent nodules and sheets of firm tan tissue covering pleural surfaces of the thoracic cavity and within pulmonary parenchyma, enlarged mesenteric lymph nodes, and intestinal ulcerations. Histopathology revealed granulomatous inflammation with intrahistiocytic yeast, consistent with Histoplasma organisms, in lung, diaphragm, mesenteric lymph nodes, intestine, and adrenal glands. Molecular analysis performed on DNA isolated from lung tissue, including conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region for the ribosomal RNA gene complex and real-time PCR targeting the gene encoding a unique region of M specific protein, identified the organism to be 100% identical to Histoplasma capsulatum with an average of 4.9 × 10 7 gene copies per gram of tissue. The present report describes histologic and molecular techniques for diagnosing histoplasmosis. Article in Journal/Newspaper Ursus arctos SAGE Publications Journal of Veterinary Diagnostic Investigation 23 4 764 769 |
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English |
description |
A 33-year-old brown bear ( Ursus arctos) was evaluated for chronic cough, partial anorexia, and lethargy in early fall of 2009. Radiographs revealed a generalized increase in interstitial density with focal lung field consolidation and air bronchograms more prevalent in the cranial lung lobes. Tracheal sputum and wash fluid grew mixed bacteria and 2 species of Candida on bacterial and fungal cultures, respectively. Serum was negative for antibodies to Aspergillus, Blastomyces, Coccidioides, and Histoplasma by semiquantitative radial immunodiffusion. Antimicrobial and antifungal treatment was administered. The bear died 1 month after entering hibernation. Gross necropsy revealed coalescent nodules and sheets of firm tan tissue covering pleural surfaces of the thoracic cavity and within pulmonary parenchyma, enlarged mesenteric lymph nodes, and intestinal ulcerations. Histopathology revealed granulomatous inflammation with intrahistiocytic yeast, consistent with Histoplasma organisms, in lung, diaphragm, mesenteric lymph nodes, intestine, and adrenal glands. Molecular analysis performed on DNA isolated from lung tissue, including conventional polymerase chain reaction (PCR) targeting the internal transcribed spacer region for the ribosomal RNA gene complex and real-time PCR targeting the gene encoding a unique region of M specific protein, identified the organism to be 100% identical to Histoplasma capsulatum with an average of 4.9 × 10 7 gene copies per gram of tissue. The present report describes histologic and molecular techniques for diagnosing histoplasmosis. |
format |
Article in Journal/Newspaper |
author |
Highland, Margaret A. Chaturvedi, Sudha Perez, Michael Steinberg, Howard Wallace, Roberta |
spellingShingle |
Highland, Margaret A. Chaturvedi, Sudha Perez, Michael Steinberg, Howard Wallace, Roberta Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
author_facet |
Highland, Margaret A. Chaturvedi, Sudha Perez, Michael Steinberg, Howard Wallace, Roberta |
author_sort |
Highland, Margaret A. |
title |
Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
title_short |
Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
title_full |
Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
title_fullStr |
Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
title_full_unstemmed |
Histologic and molecular identification of disseminated Histoplasma capsulatum in a captive brown bear ( Ursus arctos) |
title_sort |
histologic and molecular identification of disseminated histoplasma capsulatum in a captive brown bear ( ursus arctos) |
publisher |
SAGE Publications |
publishDate |
2011 |
url |
http://dx.doi.org/10.1177/1040638711406976 https://journals.sagepub.com/doi/pdf/10.1177/1040638711406976 https://journals.sagepub.com/doi/full-xml/10.1177/1040638711406976 |
genre |
Ursus arctos |
genre_facet |
Ursus arctos |
op_source |
Journal of Veterinary Diagnostic Investigation volume 23, issue 4, page 764-769 ISSN 1040-6387 1943-4936 |
op_rights |
https://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/1040638711406976 |
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Journal of Veterinary Diagnostic Investigation |
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23 |
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4 |
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764 |
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769 |
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1812181987826335744 |