Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal ( Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origi...
Published in: | Journal of Veterinary Diagnostic Investigation |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SAGE Publications
2010
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1177/104063871002200603 http://journals.sagepub.com/doi/pdf/10.1177/104063871002200603 http://journals.sagepub.com/doi/full-xml/10.1177/104063871002200603 |
id |
crsagepubl:10.1177/104063871002200603 |
---|---|
record_format |
openpolar |
spelling |
crsagepubl:10.1177/104063871002200603 2024-09-09T19:43:46+00:00 Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals Meegan, Jenny Field, Cara Sidor, Inga Romano, Tracy Casinghino, Sandra Smith, Cynthia R. Kashinsky, Lizabeth Fair, Patricia A. Bossart, Gregory Wells, Randall Dunn, J. Lawrence 2010 http://dx.doi.org/10.1177/104063871002200603 http://journals.sagepub.com/doi/pdf/10.1177/104063871002200603 http://journals.sagepub.com/doi/full-xml/10.1177/104063871002200603 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 22, issue 6, page 856-862 ISSN 1040-6387 1943-4936 journal-article 2010 crsagepubl https://doi.org/10.1177/104063871002200603 2024-08-27T04:24:13Z A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal ( Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin Brucella. Based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. For pinniped samples, specificity and sensitivity values were 77% and 67%, respectively. Hawaiian monk seal ( Monachus schauinslandi; n = 28) and bottlenose dolphin ( Tursiops truncatus; n = 48) serum samples were tested, and the results were compared with several other assays designed to detect Brucella abortus antibodies. The comparison testing revealed the marine-origin cELISA to be more sensitive than the B. abortus tests by the detection of additional positive serum samples. The newly developed cELISA is an effective serologic method for detection of the presence of antibodies against marine-origin Brucella sp. in marine mammals. Article in Journal/Newspaper harbor seal Phoca vitulina SAGE Publications Journal of Veterinary Diagnostic Investigation 22 6 856 862 |
institution |
Open Polar |
collection |
SAGE Publications |
op_collection_id |
crsagepubl |
language |
English |
description |
A competitive enzyme-linked immunosorbent assay (cELISA) was developed by using a whole-cell antigen from a marine Brucella sp. isolated from a harbor seal ( Phoca vitulina). The assay was designed to screen sera from multiple marine mammal species for the presence of antibodies against marine-origin Brucella. Based on comparisons with culture-confirmed cases, specificity and sensitivity for cetacean samples tested were 73% and 100%, respectively. For pinniped samples, specificity and sensitivity values were 77% and 67%, respectively. Hawaiian monk seal ( Monachus schauinslandi; n = 28) and bottlenose dolphin ( Tursiops truncatus; n = 48) serum samples were tested, and the results were compared with several other assays designed to detect Brucella abortus antibodies. The comparison testing revealed the marine-origin cELISA to be more sensitive than the B. abortus tests by the detection of additional positive serum samples. The newly developed cELISA is an effective serologic method for detection of the presence of antibodies against marine-origin Brucella sp. in marine mammals. |
format |
Article in Journal/Newspaper |
author |
Meegan, Jenny Field, Cara Sidor, Inga Romano, Tracy Casinghino, Sandra Smith, Cynthia R. Kashinsky, Lizabeth Fair, Patricia A. Bossart, Gregory Wells, Randall Dunn, J. Lawrence |
spellingShingle |
Meegan, Jenny Field, Cara Sidor, Inga Romano, Tracy Casinghino, Sandra Smith, Cynthia R. Kashinsky, Lizabeth Fair, Patricia A. Bossart, Gregory Wells, Randall Dunn, J. Lawrence Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
author_facet |
Meegan, Jenny Field, Cara Sidor, Inga Romano, Tracy Casinghino, Sandra Smith, Cynthia R. Kashinsky, Lizabeth Fair, Patricia A. Bossart, Gregory Wells, Randall Dunn, J. Lawrence |
author_sort |
Meegan, Jenny |
title |
Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
title_short |
Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
title_full |
Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
title_fullStr |
Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
title_full_unstemmed |
Development, Validation, and Utilization of a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies against Brucella Species in Marine Mammals |
title_sort |
development, validation, and utilization of a competitive enzyme-linked immunosorbent assay for the detection of antibodies against brucella species in marine mammals |
publisher |
SAGE Publications |
publishDate |
2010 |
url |
http://dx.doi.org/10.1177/104063871002200603 http://journals.sagepub.com/doi/pdf/10.1177/104063871002200603 http://journals.sagepub.com/doi/full-xml/10.1177/104063871002200603 |
genre |
harbor seal Phoca vitulina |
genre_facet |
harbor seal Phoca vitulina |
op_source |
Journal of Veterinary Diagnostic Investigation volume 22, issue 6, page 856-862 ISSN 1040-6387 1943-4936 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/104063871002200603 |
container_title |
Journal of Veterinary Diagnostic Investigation |
container_volume |
22 |
container_issue |
6 |
container_start_page |
856 |
op_container_end_page |
862 |
_version_ |
1809913460491812864 |