Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus
Infectious salmon anemia (ISA) is a severe disease primarily affecting commercially farmed Atlantic salmon ( Salmo salar) in seawater. The disease has been reported in portions of Canada, the United Kingdom, the Faroe Islands, and the United States. Infectious salmon anemia virus (ISAV), the causati...
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Online Access: | http://dx.doi.org/10.1177/104063870501700209 http://journals.sagepub.com/doi/pdf/10.1177/104063870501700209 |
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crsagepubl:10.1177/104063870501700209 2023-05-15T15:31:07+02:00 Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus Rolland, Jill B. Bouchard, Deborah Coll, John Winton, James R. 2005 http://dx.doi.org/10.1177/104063870501700209 http://journals.sagepub.com/doi/pdf/10.1177/104063870501700209 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 17, issue 2, page 151-157 ISSN 1040-6387 1943-4936 General Veterinary journal-article 2005 crsagepubl https://doi.org/10.1177/104063870501700209 2022-04-14T04:52:43Z Infectious salmon anemia (ISA) is a severe disease primarily affecting commercially farmed Atlantic salmon ( Salmo salar) in seawater. The disease has been reported in portions of Canada, the United Kingdom, the Faroe Islands, and the United States. Infectious salmon anemia virus (ISAV), the causative agent of ISA, has also been isolated from several asymptomatic marine and salmonid fish species. Diagnostic assays for the detection of ISAV include virus isolation in cell culture, a reverse transcriptase–PCR, an enzyme-linked immunosorbent assay, and an indirect fluorescent antibody test. Virus isolation is considered the gold standard, and 5 salmonid cell lines are known to support growth of ISAV. In this study, the relative performance of the salmon head kidney 1 (SHK-1), Atlantic salmon kidney (ASK), and CHSE-214 cell lines in detecting ISAV was evaluated using samples from both experimentally and naturally infected Atlantic salmon. Interlaboratory comparisons were conducted using a quality control–quality assurance ring test. Both the ASK and SHK-1 cell lines performed well in detecting ISAV, although the SHK-1 line was more variable in its sensitivity to infection and somewhat slower in the appearance of cytopathic effect. Relative to the SHK-1 and ASK lines, the CHSE-214 cell line performed poorly. Although the ASK line appeared to represent a good alternative to the more commonly used SHK-1 line, use of a single cell line for diagnostic assays may increase the potential for false-negative results. Thus, the SHK-1 and ASK cell lines can be used in combination to provide enhanced ability to detect ISAV. Article in Journal/Newspaper Atlantic salmon Faroe Islands Salmo salar SAGE Publications (via Crossref) Canada Faroe Islands Journal of Veterinary Diagnostic Investigation 17 2 151 157 |
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SAGE Publications (via Crossref) |
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English |
topic |
General Veterinary |
spellingShingle |
General Veterinary Rolland, Jill B. Bouchard, Deborah Coll, John Winton, James R. Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
topic_facet |
General Veterinary |
description |
Infectious salmon anemia (ISA) is a severe disease primarily affecting commercially farmed Atlantic salmon ( Salmo salar) in seawater. The disease has been reported in portions of Canada, the United Kingdom, the Faroe Islands, and the United States. Infectious salmon anemia virus (ISAV), the causative agent of ISA, has also been isolated from several asymptomatic marine and salmonid fish species. Diagnostic assays for the detection of ISAV include virus isolation in cell culture, a reverse transcriptase–PCR, an enzyme-linked immunosorbent assay, and an indirect fluorescent antibody test. Virus isolation is considered the gold standard, and 5 salmonid cell lines are known to support growth of ISAV. In this study, the relative performance of the salmon head kidney 1 (SHK-1), Atlantic salmon kidney (ASK), and CHSE-214 cell lines in detecting ISAV was evaluated using samples from both experimentally and naturally infected Atlantic salmon. Interlaboratory comparisons were conducted using a quality control–quality assurance ring test. Both the ASK and SHK-1 cell lines performed well in detecting ISAV, although the SHK-1 line was more variable in its sensitivity to infection and somewhat slower in the appearance of cytopathic effect. Relative to the SHK-1 and ASK lines, the CHSE-214 cell line performed poorly. Although the ASK line appeared to represent a good alternative to the more commonly used SHK-1 line, use of a single cell line for diagnostic assays may increase the potential for false-negative results. Thus, the SHK-1 and ASK cell lines can be used in combination to provide enhanced ability to detect ISAV. |
format |
Article in Journal/Newspaper |
author |
Rolland, Jill B. Bouchard, Deborah Coll, John Winton, James R. |
author_facet |
Rolland, Jill B. Bouchard, Deborah Coll, John Winton, James R. |
author_sort |
Rolland, Jill B. |
title |
Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
title_short |
Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
title_full |
Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
title_fullStr |
Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
title_full_unstemmed |
Combined Use of the ASK and SHK-1 Cell Lines to Enhance the Detection of Infectious Salmon Anemia Virus |
title_sort |
combined use of the ask and shk-1 cell lines to enhance the detection of infectious salmon anemia virus |
publisher |
SAGE Publications |
publishDate |
2005 |
url |
http://dx.doi.org/10.1177/104063870501700209 http://journals.sagepub.com/doi/pdf/10.1177/104063870501700209 |
geographic |
Canada Faroe Islands |
geographic_facet |
Canada Faroe Islands |
genre |
Atlantic salmon Faroe Islands Salmo salar |
genre_facet |
Atlantic salmon Faroe Islands Salmo salar |
op_source |
Journal of Veterinary Diagnostic Investigation volume 17, issue 2, page 151-157 ISSN 1040-6387 1943-4936 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/104063870501700209 |
container_title |
Journal of Veterinary Diagnostic Investigation |
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17 |
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2 |
container_start_page |
151 |
op_container_end_page |
157 |
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1766361610801119232 |