Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat
A digestion assay was validated for the detection of Trichinella larvae in walrus ( Odobenus rosmarus) meat, and appropriate samples for testing were determined using tissues from infected walruses harvested for food. Examination of muscles from 3 walruses showed that the tongue consistently contain...
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Online Access: | http://dx.doi.org/10.1177/104063870301500217 https://journals.sagepub.com/doi/pdf/10.1177/104063870301500217 |
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crsagepubl:10.1177/104063870301500217 2024-09-30T14:31:20+00:00 Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat Leclair, Daniel Forbes, Lorry B. Suppa, Sandy Gajadhar, Alvin A. 2003 http://dx.doi.org/10.1177/104063870301500217 https://journals.sagepub.com/doi/pdf/10.1177/104063870301500217 en eng SAGE Publications https://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 15, issue 2, page 188-191 ISSN 1040-6387 1943-4936 journal-article 2003 crsagepubl https://doi.org/10.1177/104063870301500217 2024-09-10T04:26:14Z A digestion assay was validated for the detection of Trichinella larvae in walrus ( Odobenus rosmarus) meat, and appropriate samples for testing were determined using tissues from infected walruses harvested for food. Examination of muscles from 3 walruses showed that the tongue consistently contained approximately 2–6 times more larvae than the pectoral and intercostal muscles. Comparison of numbers of larvae in the root, body, and apex of the tongue from 3 walruses failed to identify a predilection site within the tongue, but the apex was considered an optimal tissue because of the high larval density within the tongue and the ease of collection. All 31 spiked samples weighing 50 g each and containing between 0.1 and 0.4 larvae per gram (lpg) were correctly identified as infected, indicating that the sensitivity of this procedure is adequate for diagnostic use. A sample size of 10 g consistently detected larvae in 2 walrus tongues containing ≥0.3 lpg ( n = 40), and until additional data are available, sample sizes from individual walrus tongues should be a minimum of 10 g. This study provides the preliminary data that were used for the development of a food safety analytical protocol for the detection of Trichinella in walrus meat in arctic communities. Article in Journal/Newspaper Arctic Odobenus rosmarus walrus* SAGE Publications Arctic Journal of Veterinary Diagnostic Investigation 15 2 188 191 |
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SAGE Publications |
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English |
description |
A digestion assay was validated for the detection of Trichinella larvae in walrus ( Odobenus rosmarus) meat, and appropriate samples for testing were determined using tissues from infected walruses harvested for food. Examination of muscles from 3 walruses showed that the tongue consistently contained approximately 2–6 times more larvae than the pectoral and intercostal muscles. Comparison of numbers of larvae in the root, body, and apex of the tongue from 3 walruses failed to identify a predilection site within the tongue, but the apex was considered an optimal tissue because of the high larval density within the tongue and the ease of collection. All 31 spiked samples weighing 50 g each and containing between 0.1 and 0.4 larvae per gram (lpg) were correctly identified as infected, indicating that the sensitivity of this procedure is adequate for diagnostic use. A sample size of 10 g consistently detected larvae in 2 walrus tongues containing ≥0.3 lpg ( n = 40), and until additional data are available, sample sizes from individual walrus tongues should be a minimum of 10 g. This study provides the preliminary data that were used for the development of a food safety analytical protocol for the detection of Trichinella in walrus meat in arctic communities. |
format |
Article in Journal/Newspaper |
author |
Leclair, Daniel Forbes, Lorry B. Suppa, Sandy Gajadhar, Alvin A. |
spellingShingle |
Leclair, Daniel Forbes, Lorry B. Suppa, Sandy Gajadhar, Alvin A. Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
author_facet |
Leclair, Daniel Forbes, Lorry B. Suppa, Sandy Gajadhar, Alvin A. |
author_sort |
Leclair, Daniel |
title |
Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
title_short |
Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
title_full |
Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
title_fullStr |
Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
title_full_unstemmed |
Evaluation of a Digestion Assay and Determination of Sample Size and Tissue for the Reliable Detection of Trichinella Larvae in Walrus Meat |
title_sort |
evaluation of a digestion assay and determination of sample size and tissue for the reliable detection of trichinella larvae in walrus meat |
publisher |
SAGE Publications |
publishDate |
2003 |
url |
http://dx.doi.org/10.1177/104063870301500217 https://journals.sagepub.com/doi/pdf/10.1177/104063870301500217 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Odobenus rosmarus walrus* |
genre_facet |
Arctic Odobenus rosmarus walrus* |
op_source |
Journal of Veterinary Diagnostic Investigation volume 15, issue 2, page 188-191 ISSN 1040-6387 1943-4936 |
op_rights |
https://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/104063870301500217 |
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Journal of Veterinary Diagnostic Investigation |
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15 |
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2 |
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188 |
op_container_end_page |
191 |
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1811635941438128128 |