Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L
Piscirickettsia salmonis, the etiologic agent of salmonid rickettsial septicemia (SRS), or piscirickettsiosis, causes substantial economic losses to the salmon industry. The pathogenesis of the disease has not been fully characterized. The aim of this study is to describe the hepatic lesions associa...
Published in: | Journal of Veterinary Diagnostic Investigation |
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crsagepubl:10.1177/104063870001200610 2024-09-09T19:30:35+00:00 Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L Almendras, Felipe E. Fuentealba, I. Carmen Markham, R F. Frederick Speare, David J. 2000 http://dx.doi.org/10.1177/104063870001200610 http://journals.sagepub.com/doi/pdf/10.1177/104063870001200610 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Veterinary Diagnostic Investigation volume 12, issue 6, page 552-557 ISSN 1040-6387 1943-4936 journal-article 2000 crsagepubl https://doi.org/10.1177/104063870001200610 2024-07-29T04:26:01Z Piscirickettsia salmonis, the etiologic agent of salmonid rickettsial septicemia (SRS), or piscirickettsiosis, causes substantial economic losses to the salmon industry. The pathogenesis of the disease has not been fully characterized. The aim of this study is to describe the hepatic lesions associated with experimental P. salmonis infection in Atlantic salmon juveniles. Fish were maintained in fresh water and inoculated intraperitoneally (IP), orally, or on the gill surface with P. salmonis. A group of uninfected fish was kept as control. Liver samples from 5 fish in each inoculated group and 3 controls were collected weekly and processed for histological and immunohistochemical examination. Thickening of the liver capsule by inflammatory cells was a characteristic histologic feature of IP inoculated fish. Three weeks post-IP inoculation, 8 fish had died and 2 fish were sampled. Histological changes at this time consisted of vasculitis, presence of fibrin thrombi, vacuolated hepatocytes and focal areas of necrosis. Leukocytes containing intracytoplasmic basophilic microorganisms were seen within hepatic sinusoids. Vasculitis and intracytoplasmic vacuoles were prominent features in fish inoculated orally and on the gill surface. The presence of P. salmonis within hepatocellular vacuoles, endothelial cells, and leucocytes was confirmed by immunohistochemistry. The intracellular location of P. salmonis and the vascular damage seen in infected fish are characteristic of rickettsial infections. Histological lesions induced by experimental infection with P. salmonis using the oral and gill surface routes were similar to those observed in natural outbreaks of piscirickettsiosis. The tropism of P. salmonis for endothelial cells explains the vascular lesions observed in SRS, whereas hepatic lesions are due to ischemic necrosis and direct injury by intracytoplasmic organisms. Article in Journal/Newspaper Atlantic salmon Salmo salar SAGE Publications Journal of Veterinary Diagnostic Investigation 12 6 552 557 |
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SAGE Publications |
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English |
description |
Piscirickettsia salmonis, the etiologic agent of salmonid rickettsial septicemia (SRS), or piscirickettsiosis, causes substantial economic losses to the salmon industry. The pathogenesis of the disease has not been fully characterized. The aim of this study is to describe the hepatic lesions associated with experimental P. salmonis infection in Atlantic salmon juveniles. Fish were maintained in fresh water and inoculated intraperitoneally (IP), orally, or on the gill surface with P. salmonis. A group of uninfected fish was kept as control. Liver samples from 5 fish in each inoculated group and 3 controls were collected weekly and processed for histological and immunohistochemical examination. Thickening of the liver capsule by inflammatory cells was a characteristic histologic feature of IP inoculated fish. Three weeks post-IP inoculation, 8 fish had died and 2 fish were sampled. Histological changes at this time consisted of vasculitis, presence of fibrin thrombi, vacuolated hepatocytes and focal areas of necrosis. Leukocytes containing intracytoplasmic basophilic microorganisms were seen within hepatic sinusoids. Vasculitis and intracytoplasmic vacuoles were prominent features in fish inoculated orally and on the gill surface. The presence of P. salmonis within hepatocellular vacuoles, endothelial cells, and leucocytes was confirmed by immunohistochemistry. The intracellular location of P. salmonis and the vascular damage seen in infected fish are characteristic of rickettsial infections. Histological lesions induced by experimental infection with P. salmonis using the oral and gill surface routes were similar to those observed in natural outbreaks of piscirickettsiosis. The tropism of P. salmonis for endothelial cells explains the vascular lesions observed in SRS, whereas hepatic lesions are due to ischemic necrosis and direct injury by intracytoplasmic organisms. |
format |
Article in Journal/Newspaper |
author |
Almendras, Felipe E. Fuentealba, I. Carmen Markham, R F. Frederick Speare, David J. |
spellingShingle |
Almendras, Felipe E. Fuentealba, I. Carmen Markham, R F. Frederick Speare, David J. Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
author_facet |
Almendras, Felipe E. Fuentealba, I. Carmen Markham, R F. Frederick Speare, David J. |
author_sort |
Almendras, Felipe E. |
title |
Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
title_short |
Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
title_full |
Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
title_fullStr |
Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
title_full_unstemmed |
Pathogenesis of Liver Lesions Caused by Experimental Infection with Piscirickettsia Salmonis in Juvenile Atlantic Salmon, Salmo Salar L |
title_sort |
pathogenesis of liver lesions caused by experimental infection with piscirickettsia salmonis in juvenile atlantic salmon, salmo salar l |
publisher |
SAGE Publications |
publishDate |
2000 |
url |
http://dx.doi.org/10.1177/104063870001200610 http://journals.sagepub.com/doi/pdf/10.1177/104063870001200610 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Journal of Veterinary Diagnostic Investigation volume 12, issue 6, page 552-557 ISSN 1040-6387 1943-4936 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/104063870001200610 |
container_title |
Journal of Veterinary Diagnostic Investigation |
container_volume |
12 |
container_issue |
6 |
container_start_page |
552 |
op_container_end_page |
557 |
_version_ |
1809899579279147008 |