The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase
In nature, an interface between dissimilar tissues is often bridged by a graded zone, and provides functional properties at a whole organ level. A perfect example is a “biological interphase” between stratified cementum and dentin of a narwhal tooth. This study highlights the graded structural, mech...
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crsagepubl:10.1177/0954411914547553 2023-05-15T17:13:06+02:00 The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase Grandfield, Kathryn Chattah, Netta Lev-Tov Djomehri, Sabra Eidelmann, Naomi Eichmiller, Frederick C Webb, Samuel Schuck, P James Nweeia, Martin Ho, Sunita P 2014 http://dx.doi.org/10.1177/0954411914547553 http://journals.sagepub.com/doi/pdf/10.1177/0954411914547553 http://journals.sagepub.com/doi/full-xml/10.1177/0954411914547553 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine volume 228, issue 8, page 754-767 ISSN 0954-4119 2041-3033 Mechanical Engineering General Medicine journal-article 2014 crsagepubl https://doi.org/10.1177/0954411914547553 2022-04-14T04:38:31Z In nature, an interface between dissimilar tissues is often bridged by a graded zone, and provides functional properties at a whole organ level. A perfect example is a “biological interphase” between stratified cementum and dentin of a narwhal tooth. This study highlights the graded structural, mechanical, and chemical natural characteristics of a biological interphase known as the cementum–dentin junction layer and their effect in resisting mechanical loads. From a structural perspective, light and electron microscopy techniques illustrated the layer as a wide 1000–2000 μm graded zone consisting of higher density continuous collagen fiber bundles from the surface of cementum to dentin, that parallels hygroscopic 50–100 μm wide collagenous region in human teeth. The role of collagen fibers was evident under compression testing during which the layer deformed more compared to cementum and dentin. This behavior is reflected through site-specific nanoindentation indicating a lower elastic modulus of 2.2 ± 0.5 GPa for collagen fiber bundle compared to 3 ± 0.4 GPa for mineralized regions in the layer. Similarly, microindentation technique illustrated lower hardness values of 0.36 ± 0.05 GPa, 0.33 ± 0.03 GPa, and 0.3 ± 0.07 GPa for cementum, dentin, and cementum–dentin layer, respectively. Biochemical analyses including Raman spectroscopy and synchrotron-source microprobe X-ray fluorescence demonstrated a graded composition across the interface, including a decrease in mineral-to-matrix and phosphate-to-carbonate ratios, as well as the presence of tidemark-like bands with Zn. Understanding the structure–function relationships of wider tissue interfaces can provide insights into natural tissue and organ function. Article in Journal/Newspaper Monodon monoceros narwhal* SAGE Publications (via Crossref) Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine 228 8 754 767 |
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SAGE Publications (via Crossref) |
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Mechanical Engineering General Medicine |
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Mechanical Engineering General Medicine Grandfield, Kathryn Chattah, Netta Lev-Tov Djomehri, Sabra Eidelmann, Naomi Eichmiller, Frederick C Webb, Samuel Schuck, P James Nweeia, Martin Ho, Sunita P The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
topic_facet |
Mechanical Engineering General Medicine |
description |
In nature, an interface between dissimilar tissues is often bridged by a graded zone, and provides functional properties at a whole organ level. A perfect example is a “biological interphase” between stratified cementum and dentin of a narwhal tooth. This study highlights the graded structural, mechanical, and chemical natural characteristics of a biological interphase known as the cementum–dentin junction layer and their effect in resisting mechanical loads. From a structural perspective, light and electron microscopy techniques illustrated the layer as a wide 1000–2000 μm graded zone consisting of higher density continuous collagen fiber bundles from the surface of cementum to dentin, that parallels hygroscopic 50–100 μm wide collagenous region in human teeth. The role of collagen fibers was evident under compression testing during which the layer deformed more compared to cementum and dentin. This behavior is reflected through site-specific nanoindentation indicating a lower elastic modulus of 2.2 ± 0.5 GPa for collagen fiber bundle compared to 3 ± 0.4 GPa for mineralized regions in the layer. Similarly, microindentation technique illustrated lower hardness values of 0.36 ± 0.05 GPa, 0.33 ± 0.03 GPa, and 0.3 ± 0.07 GPa for cementum, dentin, and cementum–dentin layer, respectively. Biochemical analyses including Raman spectroscopy and synchrotron-source microprobe X-ray fluorescence demonstrated a graded composition across the interface, including a decrease in mineral-to-matrix and phosphate-to-carbonate ratios, as well as the presence of tidemark-like bands with Zn. Understanding the structure–function relationships of wider tissue interfaces can provide insights into natural tissue and organ function. |
format |
Article in Journal/Newspaper |
author |
Grandfield, Kathryn Chattah, Netta Lev-Tov Djomehri, Sabra Eidelmann, Naomi Eichmiller, Frederick C Webb, Samuel Schuck, P James Nweeia, Martin Ho, Sunita P |
author_facet |
Grandfield, Kathryn Chattah, Netta Lev-Tov Djomehri, Sabra Eidelmann, Naomi Eichmiller, Frederick C Webb, Samuel Schuck, P James Nweeia, Martin Ho, Sunita P |
author_sort |
Grandfield, Kathryn |
title |
The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
title_short |
The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
title_full |
The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
title_fullStr |
The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
title_full_unstemmed |
The narwhal ( Monodon monoceros ) cementum–dentin junction: A functionally graded biointerphase |
title_sort |
narwhal ( monodon monoceros ) cementum–dentin junction: a functionally graded biointerphase |
publisher |
SAGE Publications |
publishDate |
2014 |
url |
http://dx.doi.org/10.1177/0954411914547553 http://journals.sagepub.com/doi/pdf/10.1177/0954411914547553 http://journals.sagepub.com/doi/full-xml/10.1177/0954411914547553 |
genre |
Monodon monoceros narwhal* |
genre_facet |
Monodon monoceros narwhal* |
op_source |
Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine volume 228, issue 8, page 754-767 ISSN 0954-4119 2041-3033 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/0954411914547553 |
container_title |
Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine |
container_volume |
228 |
container_issue |
8 |
container_start_page |
754 |
op_container_end_page |
767 |
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1766070009986023424 |