Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes
Many studies have reported on the conversion of natural resources into xenografts with hydroxyapatite (HA) as major component, but the extraction of biphasic calcium phosphate (HA/β-TCP) from animal bones and transformation into bone graft substitutes are rarely reported. In this research, two kinds...
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crsagepubl:10.1177/08853282221111969 2024-09-09T20:05:56+00:00 Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes Zhu, Qingfeng Chen, Tong Xia, Jinfeng Jiang, Danyu Wang, Shaohai Zhang, Yuntong National Natural Science Foundation of China 2022 http://dx.doi.org/10.1177/08853282221111969 http://journals.sagepub.com/doi/pdf/10.1177/08853282221111969 http://journals.sagepub.com/doi/full-xml/10.1177/08853282221111969 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Journal of Biomaterials Applications volume 37, issue 4, page 600-613 ISSN 0885-3282 1530-8022 journal-article 2022 crsagepubl https://doi.org/10.1177/08853282221111969 2024-08-05T04:40:50Z Many studies have reported on the conversion of natural resources into xenografts with hydroxyapatite (HA) as major component, but the extraction of biphasic calcium phosphate (HA/β-TCP) from animal bones and transformation into bone graft substitutes are rarely reported. In this research, two kinds of fish bones were made into granular porous biphasic calcium phosphate bone graft substitutes with particle sizes between 500 to 1000 μm through a series of preparation procedures ( Salmo salar calcined at 900°C named Sa900 and Anoplopoma fimbria calcined at 800°C named An800). The chemical composition was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The morphology and porous structure of the scaffolds were comparatively analyzed by scanning electron microscopy (SEM) and mercury porosimeter. The specific surface area of materials was measured by the nitrogen adsorption technique based on BET theory. Cytotoxicity and ectopic osteogenesis were also carried out to investigate the biocompatibility and osteoinductive potential of these materials. The results showed that both fishbone-derived scaffolds were composed of HA and β-TCP with different proportions, and numerous interconnected pores with different sizes were observed at the surface of materials. An800 had higher total porosity reaching 74.8% with higher interconnectivity and micropores mostly distributed at 0.27 μm and 0.12 μm, while Sa900 had a higher specific surface area and higher intraparticle porosity with nanopores mostly distributed at 0.07 μm. CCK-8 assays and Live/dead staining demonstrated excellent biocompatibility. Material-induced osteoid formation were observed on the interface of both internal pores and periphery of materials after implantation in muscle pouch of Wistar rats for 8 weeks which indicated some extent of osteoinductive potential of materials. The possible mechanism of material-induced osteogenesis and the effects of chemical composition, surface topography, and spatial structure on ... Article in Journal/Newspaper Salmo salar SAGE Publications Journal of Biomaterials Applications 088532822211119 |
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Many studies have reported on the conversion of natural resources into xenografts with hydroxyapatite (HA) as major component, but the extraction of biphasic calcium phosphate (HA/β-TCP) from animal bones and transformation into bone graft substitutes are rarely reported. In this research, two kinds of fish bones were made into granular porous biphasic calcium phosphate bone graft substitutes with particle sizes between 500 to 1000 μm through a series of preparation procedures ( Salmo salar calcined at 900°C named Sa900 and Anoplopoma fimbria calcined at 800°C named An800). The chemical composition was characterized by X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). The morphology and porous structure of the scaffolds were comparatively analyzed by scanning electron microscopy (SEM) and mercury porosimeter. The specific surface area of materials was measured by the nitrogen adsorption technique based on BET theory. Cytotoxicity and ectopic osteogenesis were also carried out to investigate the biocompatibility and osteoinductive potential of these materials. The results showed that both fishbone-derived scaffolds were composed of HA and β-TCP with different proportions, and numerous interconnected pores with different sizes were observed at the surface of materials. An800 had higher total porosity reaching 74.8% with higher interconnectivity and micropores mostly distributed at 0.27 μm and 0.12 μm, while Sa900 had a higher specific surface area and higher intraparticle porosity with nanopores mostly distributed at 0.07 μm. CCK-8 assays and Live/dead staining demonstrated excellent biocompatibility. Material-induced osteoid formation were observed on the interface of both internal pores and periphery of materials after implantation in muscle pouch of Wistar rats for 8 weeks which indicated some extent of osteoinductive potential of materials. The possible mechanism of material-induced osteogenesis and the effects of chemical composition, surface topography, and spatial structure on ... |
author2 |
National Natural Science Foundation of China |
format |
Article in Journal/Newspaper |
author |
Zhu, Qingfeng Chen, Tong Xia, Jinfeng Jiang, Danyu Wang, Shaohai Zhang, Yuntong |
spellingShingle |
Zhu, Qingfeng Chen, Tong Xia, Jinfeng Jiang, Danyu Wang, Shaohai Zhang, Yuntong Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
author_facet |
Zhu, Qingfeng Chen, Tong Xia, Jinfeng Jiang, Danyu Wang, Shaohai Zhang, Yuntong |
author_sort |
Zhu, Qingfeng |
title |
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
title_short |
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
title_full |
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
title_fullStr |
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
title_full_unstemmed |
Preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
title_sort |
preparation and characterization of two novel osteoinductive fishbone-derived biphasic calcium phosphate bone graft substitutes |
publisher |
SAGE Publications |
publishDate |
2022 |
url |
http://dx.doi.org/10.1177/08853282221111969 http://journals.sagepub.com/doi/pdf/10.1177/08853282221111969 http://journals.sagepub.com/doi/full-xml/10.1177/08853282221111969 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Journal of Biomaterials Applications volume 37, issue 4, page 600-613 ISSN 0885-3282 1530-8022 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/08853282221111969 |
container_title |
Journal of Biomaterials Applications |
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088532822211119 |
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1809938337329315840 |