A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.)
The uptake and handling of Vibrio salmonicida in phagocytes of the head kidney of Atlantic salmon (Salmo salar L.) were evaluated by light and electron microscopy, including in situ identification of the bacterium by immunolabeling at the light microscopical and the ultrastructural level. Fish were...
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Language: | English |
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SAGE Publications
1996
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Online Access: | http://dx.doi.org/10.1177/030098589603300106 http://journals.sagepub.com/doi/pdf/10.1177/030098589603300106 |
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crsagepubl:10.1177/030098589603300106 2024-05-19T07:37:39+00:00 A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) Brattgjerd, S. Evensen, Ø. 1996 http://dx.doi.org/10.1177/030098589603300106 http://journals.sagepub.com/doi/pdf/10.1177/030098589603300106 en eng SAGE Publications http://journals.sagepub.com/page/policies/text-and-data-mining-license Veterinary Pathology volume 33, issue 1, page 55-65 ISSN 0300-9858 1544-2217 journal-article 1996 crsagepubl https://doi.org/10.1177/030098589603300106 2024-04-25T08:12:16Z The uptake and handling of Vibrio salmonicida in phagocytes of the head kidney of Atlantic salmon (Salmo salar L.) were evaluated by light and electron microscopy, including in situ identification of the bacterium by immunolabeling at the light microscopical and the ultrastructural level. Fish were injected with live bacteria, and 4, 24, 48, and 72 hours after inoculation, samples were collected after perfusion fixation. Morphologically, the most prominent change in the course of the experiment was an increasing number of intrasinusoidal, endothelial cell-adherent phagocytes and the elevated number of interstitial melanomacrophages. Immunohistochemically, bacterial antigens were initially identified in intrasinusoidal phagocytes, and at 24 hours postinfection in endothelium-adherent phagocytes and intrasinusoidal melanomacrophages. Later, (48 and 72 hours postinfection), the interstitial melanomacrophages were also found to harbor bacterial antigen. Ultrastructurally, bacteria were identified in phagosomes in intrasinusoidal phagocytes, and morphological findings also indicated an increased cellular degradation, including autophagocytosis. Immunoelectron microscopy indicated that bacterial antigens were associated with melanomacrophages, specifically in their electron-dense cytoplasmic granules. These findings indicate that intrasinusoidal phagocytes and melanomacrophages participate in the rapid and active clearance of particulate material from the circulation, i.e., pathogenic microorganisms, and in the scavenging of cellular degradation products. The process of formation of melanomacrophages and their possible function is discussed. Article in Journal/Newspaper Atlantic salmon Salmo salar SAGE Publications Veterinary Pathology 33 1 55 65 |
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SAGE Publications |
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language |
English |
description |
The uptake and handling of Vibrio salmonicida in phagocytes of the head kidney of Atlantic salmon (Salmo salar L.) were evaluated by light and electron microscopy, including in situ identification of the bacterium by immunolabeling at the light microscopical and the ultrastructural level. Fish were injected with live bacteria, and 4, 24, 48, and 72 hours after inoculation, samples were collected after perfusion fixation. Morphologically, the most prominent change in the course of the experiment was an increasing number of intrasinusoidal, endothelial cell-adherent phagocytes and the elevated number of interstitial melanomacrophages. Immunohistochemically, bacterial antigens were initially identified in intrasinusoidal phagocytes, and at 24 hours postinfection in endothelium-adherent phagocytes and intrasinusoidal melanomacrophages. Later, (48 and 72 hours postinfection), the interstitial melanomacrophages were also found to harbor bacterial antigen. Ultrastructurally, bacteria were identified in phagosomes in intrasinusoidal phagocytes, and morphological findings also indicated an increased cellular degradation, including autophagocytosis. Immunoelectron microscopy indicated that bacterial antigens were associated with melanomacrophages, specifically in their electron-dense cytoplasmic granules. These findings indicate that intrasinusoidal phagocytes and melanomacrophages participate in the rapid and active clearance of particulate material from the circulation, i.e., pathogenic microorganisms, and in the scavenging of cellular degradation products. The process of formation of melanomacrophages and their possible function is discussed. |
format |
Article in Journal/Newspaper |
author |
Brattgjerd, S. Evensen, Ø. |
spellingShingle |
Brattgjerd, S. Evensen, Ø. A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
author_facet |
Brattgjerd, S. Evensen, Ø. |
author_sort |
Brattgjerd, S. |
title |
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
title_short |
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
title_full |
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
title_fullStr |
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
title_full_unstemmed |
A Sequential Light Microscopic and Ultrastructural Study on the Uptake and Handling of Vibrio salmonicida in Phagocytes of the Head Kidney in Experimentally Infected Atlantic Salmon ( Salmo salar L.) |
title_sort |
sequential light microscopic and ultrastructural study on the uptake and handling of vibrio salmonicida in phagocytes of the head kidney in experimentally infected atlantic salmon ( salmo salar l.) |
publisher |
SAGE Publications |
publishDate |
1996 |
url |
http://dx.doi.org/10.1177/030098589603300106 http://journals.sagepub.com/doi/pdf/10.1177/030098589603300106 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Veterinary Pathology volume 33, issue 1, page 55-65 ISSN 0300-9858 1544-2217 |
op_rights |
http://journals.sagepub.com/page/policies/text-and-data-mining-license |
op_doi |
https://doi.org/10.1177/030098589603300106 |
container_title |
Veterinary Pathology |
container_volume |
33 |
container_issue |
1 |
container_start_page |
55 |
op_container_end_page |
65 |
_version_ |
1799477000272871424 |