Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading
Omega-3 ( ω 3 or n -3) long-chain polyunsaturated fatty acids (PUFA), including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to have originated almost exclusively from aquatic (mostly marine) single...
Published in: | Philosophical Transactions of the Royal Society B: Biological Sciences |
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crroyalsociety:10.1098/rstb.2019.0654 2024-10-06T13:51:14+00:00 Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading Kabeya, Naoki Gür, İbrahim Oboh, Angela Evjemo, Jan Ove Malzahn, Arne M. Hontoria, Francisco Navarro, Juan C. Monroig, Óscar Japan Society for the Promotion of Science Ministerio de Ciencia, Innovación y Universidades Norges Forskningsråd Commonwealth Scholarship Commission Ministry of National Education, Republic of Turkey 2020 http://dx.doi.org/10.1098/rstb.2019.0654 https://royalsocietypublishing.org/doi/pdf/10.1098/rstb.2019.0654 https://royalsocietypublishing.org/doi/full-xml/10.1098/rstb.2019.0654 en eng The Royal Society https://royalsociety.org/-/media/journals/author/Licence-to-Publish-20062019-final.pdf https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Philosophical Transactions of the Royal Society B: Biological Sciences volume 375, issue 1804, page 20190654 ISSN 0962-8436 1471-2970 journal-article 2020 crroyalsociety https://doi.org/10.1098/rstb.2019.0654 2024-09-17T04:34:44Z Omega-3 ( ω 3 or n -3) long-chain polyunsaturated fatty acids (PUFA), including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to have originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases ( ω x ) that enables them to endogenously produce n -3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ω x enzymes from the common ragworm, Hediste diversicolor , a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ω x demonstrated unique desaturation capacities. An ω 3 desaturase mediated the conversion of n -6 fatty acid substrates into their corresponding n -3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω 6 and ω 3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. Article in Journal/Newspaper North Atlantic The Royal Society Philosophical Transactions of the Royal Society B: Biological Sciences 375 1804 20190654 |
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Open Polar |
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The Royal Society |
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crroyalsociety |
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English |
description |
Omega-3 ( ω 3 or n -3) long-chain polyunsaturated fatty acids (PUFA), including eicosapentaenoic acid and docosahexaenoic acid (DHA), play physiologically important roles in vertebrates. These compounds have long been believed to have originated almost exclusively from aquatic (mostly marine) single-cell organisms. Yet, a recent study has discovered that many invertebrates possess a type of enzymes called methyl-end desaturases ( ω x ) that enables them to endogenously produce n -3 long-chain PUFA and could make a significant contribution to production of these compounds in the marine environment. Polychaetes are major components of benthic fauna and thus important to maintain a robust food web as a recycler of organic matter and a prey item for higher trophic level species like fish. In the present study, we investigated the ω x enzymes from the common ragworm, Hediste diversicolor , a common inhabitant in sedimentary littoral ecosystems of the North Atlantic. Functional assays of the H. diversicolor ω x demonstrated unique desaturation capacities. An ω 3 desaturase mediated the conversion of n -6 fatty acid substrates into their corresponding n -3 products including DHA. A further enzyme possessed unique regioselectivities combining both ω 6 and ω 3 desaturase activities. These results illustrate that the long-chain PUFA biosynthetic enzymatic machinery of aquatic invertebrates such as polychaetes is highly diverse and clarify that invertebrates can be major contributors to fatty acid trophic upgrading in aquatic food webs. This article is part of the theme issue ‘The next horizons for lipids as ‘trophic biomarkers’: evidence and significance of consumer modification of dietary fatty acids’. |
author2 |
Japan Society for the Promotion of Science Ministerio de Ciencia, Innovación y Universidades Norges Forskningsråd Commonwealth Scholarship Commission Ministry of National Education, Republic of Turkey |
format |
Article in Journal/Newspaper |
author |
Kabeya, Naoki Gür, İbrahim Oboh, Angela Evjemo, Jan Ove Malzahn, Arne M. Hontoria, Francisco Navarro, Juan C. Monroig, Óscar |
spellingShingle |
Kabeya, Naoki Gür, İbrahim Oboh, Angela Evjemo, Jan Ove Malzahn, Arne M. Hontoria, Francisco Navarro, Juan C. Monroig, Óscar Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
author_facet |
Kabeya, Naoki Gür, İbrahim Oboh, Angela Evjemo, Jan Ove Malzahn, Arne M. Hontoria, Francisco Navarro, Juan C. Monroig, Óscar |
author_sort |
Kabeya, Naoki |
title |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_short |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_full |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_fullStr |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_full_unstemmed |
Unique fatty acid desaturase capacities uncovered in Hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
title_sort |
unique fatty acid desaturase capacities uncovered in hediste diversicolor illustrate the roles of aquatic invertebrates in trophic upgrading |
publisher |
The Royal Society |
publishDate |
2020 |
url |
http://dx.doi.org/10.1098/rstb.2019.0654 https://royalsocietypublishing.org/doi/pdf/10.1098/rstb.2019.0654 https://royalsocietypublishing.org/doi/full-xml/10.1098/rstb.2019.0654 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Philosophical Transactions of the Royal Society B: Biological Sciences volume 375, issue 1804, page 20190654 ISSN 0962-8436 1471-2970 |
op_rights |
https://royalsociety.org/-/media/journals/author/Licence-to-Publish-20062019-final.pdf https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rstb.2019.0654 |
container_title |
Philosophical Transactions of the Royal Society B: Biological Sciences |
container_volume |
375 |
container_issue |
1804 |
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20190654 |
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1812179430117736448 |