Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for f...
Published in: | Proceedings of the Royal Society B: Biological Sciences |
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Online Access: | http://dx.doi.org/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/full-xml/10.1098/rspb.2021.0020 |
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crroyalsociety:10.1098/rspb.2021.0020 2024-05-12T08:00:15+00:00 Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla Novo Nordisk Fonden Danmarks Grundforskningsfond Arts and Humanities Research Council Social Sciences and Humanities Research Council of Canada H2020 European Research Council 2021 http://dx.doi.org/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/full-xml/10.1098/rspb.2021.0020 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Proceedings of the Royal Society B: Biological Sciences volume 288, issue 1954, page 20210020 ISSN 0962-8452 1471-2954 General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine journal-article 2021 crroyalsociety https://doi.org/10.1098/rspb.2021.0020 2024-04-18T08:22:01Z The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. Article in Journal/Newspaper Arctic permafrost Alaska The Royal Society Arctic Pacific Sockeye ENVELOPE(-130.143,-130.143,54.160,54.160) Proceedings of the Royal Society B: Biological Sciences 288 1954 20210020 |
institution |
Open Polar |
collection |
The Royal Society |
op_collection_id |
crroyalsociety |
language |
English |
topic |
General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
spellingShingle |
General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
topic_facet |
General Agricultural and Biological Sciences General Environmental Science General Immunology and Microbiology General Biochemistry, Genetics and Molecular Biology General Medicine |
description |
The domestic dog has inhabited the anthropogenic niche for at least 15 000 years, but despite their impact on human strategies, the lives of dogs and their interactions with humans have only recently become a subject of interest to archaeologists. In the Arctic, dogs rely exclusively on humans for food during the winter, and while stable isotope analyses have revealed dietary similarities at some sites, deciphering the details of provisioning strategies have been challenging. In this study, we apply zooarchaeology by mass spectrometry (ZooMS) and liquid chromatography tandem mass spectrometry to dog palaeofaeces to investigate protein preservation in this highly degradable material and obtain information about the diet of domestic dogs at the Nunalleq site, Alaska. We identify a suite of digestive and metabolic proteins from the host species, demonstrating the utility of this material as a novel and viable substrate for the recovery of gastrointestinal proteomes. The recovered proteins revealed that the Nunalleq dogs consumed a range of Pacific salmon species (coho, chum, chinook and sockeye) and that the consumed tissues derived from muscle and bone tissues as well as roe and guts. Overall, the study demonstrated the viability of permafrost-preserved palaeofaeces as a unique source of host and dietary proteomes. |
author2 |
Novo Nordisk Fonden Danmarks Grundforskningsfond Arts and Humanities Research Council Social Sciences and Humanities Research Council of Canada H2020 European Research Council |
format |
Article in Journal/Newspaper |
author |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_facet |
Runge, Anne Kathrine W. Hendy, Jessica Richter, Kristine K. Masson-MacLean, Edouard Britton, Kate Mackie, Meaghan McGrath, Krista Collins, Matthew Cappellini, Enrico Speller, Camilla |
author_sort |
Runge, Anne Kathrine W. |
title |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_short |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_fullStr |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_full_unstemmed |
Palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
title_sort |
palaeoproteomic analyses of dog palaeofaeces reveal a preserved dietary and host digestive proteome |
publisher |
The Royal Society |
publishDate |
2021 |
url |
http://dx.doi.org/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2021.0020 https://royalsocietypublishing.org/doi/full-xml/10.1098/rspb.2021.0020 |
long_lat |
ENVELOPE(-130.143,-130.143,54.160,54.160) |
geographic |
Arctic Pacific Sockeye |
geographic_facet |
Arctic Pacific Sockeye |
genre |
Arctic permafrost Alaska |
genre_facet |
Arctic permafrost Alaska |
op_source |
Proceedings of the Royal Society B: Biological Sciences volume 288, issue 1954, page 20210020 ISSN 0962-8452 1471-2954 |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rspb.2021.0020 |
container_title |
Proceedings of the Royal Society B: Biological Sciences |
container_volume |
288 |
container_issue |
1954 |
container_start_page |
20210020 |
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1798842038315122688 |