Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus
Quantitative trait loci (QTL) studies have indicated growth hormone receptor (GHR) as a candidate gene affecting cattle milk yield and composition. In order to characterize genetic variation at GHR in cattle, we studied European and East African breeds with different histories of selection, and Bos...
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crroyalsociety:10.1098/rspb.2008.0181 2024-06-02T08:13:38+00:00 Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus Varvio, S.-L Iso-Touru, T Kantanen, J Viitala, S Tapio, I Mäki-Tanila, A Zerabruk, M Vilkki, J 2008 http://dx.doi.org/10.1098/rspb.2008.0181 https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2008.0181 https://royalsocietypublishing.org/doi/full-xml/10.1098/rspb.2008.0181 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Proceedings of the Royal Society B: Biological Sciences volume 275, issue 1642, page 1525-1534 ISSN 0962-8452 1471-2954 journal-article 2008 crroyalsociety https://doi.org/10.1098/rspb.2008.0181 2024-05-07T14:16:19Z Quantitative trait loci (QTL) studies have indicated growth hormone receptor (GHR) as a candidate gene affecting cattle milk yield and composition. In order to characterize genetic variation at GHR in cattle, we studied European and East African breeds with different histories of selection, and Bos grunniens , Ovis aries , Sus scrofa , Bison bison and Rangifer tarandus as references. We sequenced most of the cytoplasmic domain (900 bp of exon 10), 89 bp of exon 8, including the putative causative mutation for the QTL effect, and 390 bp of intron 8 for comparison. In the cytoplasmic domain, seven synonymous and seven non-synonymous single nucleotide polymorphisms (SNP) were identified in cattle. Three non-synonymous SNPs were found in sheep and one synonymous SNP in yak, while other studied species were monomorphic. Three major haplotypes were observed, one unique to African breeds, one unique to European breeds and one shared. Bison and yak haplotypes are derivatives of the European haplotype lineage. Most of the exon 10 non-synonymous cattle SNPs appear at phylogenetically highly conserved sites. The polymorphisms in exon 10 cluster around a ruminant-specific tyrosine residue, suggesting that this site may act as an additional signalling domain of GHR in ruminants. Alternative explanations for the persistent polymorphism include balancing selection, hitch-hiking, pleiotropic or sexually antagonistic fitness effects or relaxed functional constraints. Article in Journal/Newspaper Rangifer tarandus The Royal Society Proceedings of the Royal Society B: Biological Sciences 275 1642 1525 1534 |
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crroyalsociety |
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English |
description |
Quantitative trait loci (QTL) studies have indicated growth hormone receptor (GHR) as a candidate gene affecting cattle milk yield and composition. In order to characterize genetic variation at GHR in cattle, we studied European and East African breeds with different histories of selection, and Bos grunniens , Ovis aries , Sus scrofa , Bison bison and Rangifer tarandus as references. We sequenced most of the cytoplasmic domain (900 bp of exon 10), 89 bp of exon 8, including the putative causative mutation for the QTL effect, and 390 bp of intron 8 for comparison. In the cytoplasmic domain, seven synonymous and seven non-synonymous single nucleotide polymorphisms (SNP) were identified in cattle. Three non-synonymous SNPs were found in sheep and one synonymous SNP in yak, while other studied species were monomorphic. Three major haplotypes were observed, one unique to African breeds, one unique to European breeds and one shared. Bison and yak haplotypes are derivatives of the European haplotype lineage. Most of the exon 10 non-synonymous cattle SNPs appear at phylogenetically highly conserved sites. The polymorphisms in exon 10 cluster around a ruminant-specific tyrosine residue, suggesting that this site may act as an additional signalling domain of GHR in ruminants. Alternative explanations for the persistent polymorphism include balancing selection, hitch-hiking, pleiotropic or sexually antagonistic fitness effects or relaxed functional constraints. |
format |
Article in Journal/Newspaper |
author |
Varvio, S.-L Iso-Touru, T Kantanen, J Viitala, S Tapio, I Mäki-Tanila, A Zerabruk, M Vilkki, J |
spellingShingle |
Varvio, S.-L Iso-Touru, T Kantanen, J Viitala, S Tapio, I Mäki-Tanila, A Zerabruk, M Vilkki, J Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
author_facet |
Varvio, S.-L Iso-Touru, T Kantanen, J Viitala, S Tapio, I Mäki-Tanila, A Zerabruk, M Vilkki, J |
author_sort |
Varvio, S.-L |
title |
Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
title_short |
Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
title_full |
Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
title_fullStr |
Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
title_full_unstemmed |
Molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
title_sort |
molecular anatomy of the cytoplasmic domain of bovine growth hormone receptor, a quantitative trait locus |
publisher |
The Royal Society |
publishDate |
2008 |
url |
http://dx.doi.org/10.1098/rspb.2008.0181 https://royalsocietypublishing.org/doi/pdf/10.1098/rspb.2008.0181 https://royalsocietypublishing.org/doi/full-xml/10.1098/rspb.2008.0181 |
genre |
Rangifer tarandus |
genre_facet |
Rangifer tarandus |
op_source |
Proceedings of the Royal Society B: Biological Sciences volume 275, issue 1642, page 1525-1534 ISSN 0962-8452 1471-2954 |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rspb.2008.0181 |
container_title |
Proceedings of the Royal Society B: Biological Sciences |
container_volume |
275 |
container_issue |
1642 |
container_start_page |
1525 |
op_container_end_page |
1534 |
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1800737207460298752 |