The crystal structure of myoglobin II. Finback whale myoglobin
Myoglobin of Balaenoptera physalus (finback whale) normally forms orthorhombic crystals with space group P2 1 2 1 2 and cell dimensions a = 97·4, b = 39·8, c = 42·5 Å; the unit cell contains four molecules. The intensities of the reflexions in the three principal zones have been used to compute Patt...
Published in: | Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
The Royal Society
1956
|
Subjects: | |
Online Access: | http://dx.doi.org/10.1098/rspa.1956.0176 https://royalsocietypublishing.org/doi/pdf/10.1098/rspa.1956.0176 |
id |
crroyalsociety:10.1098/rspa.1956.0176 |
---|---|
record_format |
openpolar |
spelling |
crroyalsociety:10.1098/rspa.1956.0176 2024-06-02T08:04:00+00:00 The crystal structure of myoglobin II. Finback whale myoglobin 1956 http://dx.doi.org/10.1098/rspa.1956.0176 https://royalsocietypublishing.org/doi/pdf/10.1098/rspa.1956.0176 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences volume 237, issue 1209, page 255-276 ISSN 0080-4630 2053-9169 journal-article 1956 crroyalsociety https://doi.org/10.1098/rspa.1956.0176 2024-05-07T14:16:45Z Myoglobin of Balaenoptera physalus (finback whale) normally forms orthorhombic crystals with space group P2 1 2 1 2 and cell dimensions a = 97·4, b = 39·8, c = 42·5 Å; the unit cell contains four molecules. The intensities of the reflexions in the three principal zones have been used to compute Patterson projections, which exhibit rod-like features parallel to z . These rods are 10 Å apart in approximately hexagonal packing and have nodes at 5 Å intervals; they are taken to be the vector equivalents of a set of quasi-parallel polypeptide chains with the same orientation in real space, the mean chain direction being about the same in all four molecules in the cell. Further evidence for this conclusion is derived from the radial distribution of intensities, which is anisotropic in the sense that there is a marked preponderance of 10 Å reflexions in the [001] zone; and from the absolute magnitudes of the reflexions, which are compatible with the hypothesis that 25 to 50% of the molecule is made up of parallel polypeptide chains. If the chains were only approximately parallel they might account for a larger proportion of the molecule. The changes in the low-order reflexions produced by variation in the electron density of the suspension medium have been used to find the position of the molecules in the unit cell; confirmation of the results is found in the Patterson projections. The orientation of the haem group relative to the crystal axes is derived from measurements of paramagnetic resonance (Bennett & Ingram 1956 a ) and of optical dichroism. It is concluded that the plane of the haem group is inclined at 41° to the mean chain direction. Unambiguous information about the structure of the molecule will only be obtained by the isomorphous replacement method; meanwhile the above results, taken in conjunction with others derived from different crystal forms of myoglobin, have been used to discuss some plausible models. The most favoured is 42 Å long (in the chain direction) and has a cross-section of 25 x 35 Å. ... Article in Journal/Newspaper Balaenoptera physalus The Royal Society Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences 237 1209 255 276 |
institution |
Open Polar |
collection |
The Royal Society |
op_collection_id |
crroyalsociety |
language |
English |
description |
Myoglobin of Balaenoptera physalus (finback whale) normally forms orthorhombic crystals with space group P2 1 2 1 2 and cell dimensions a = 97·4, b = 39·8, c = 42·5 Å; the unit cell contains four molecules. The intensities of the reflexions in the three principal zones have been used to compute Patterson projections, which exhibit rod-like features parallel to z . These rods are 10 Å apart in approximately hexagonal packing and have nodes at 5 Å intervals; they are taken to be the vector equivalents of a set of quasi-parallel polypeptide chains with the same orientation in real space, the mean chain direction being about the same in all four molecules in the cell. Further evidence for this conclusion is derived from the radial distribution of intensities, which is anisotropic in the sense that there is a marked preponderance of 10 Å reflexions in the [001] zone; and from the absolute magnitudes of the reflexions, which are compatible with the hypothesis that 25 to 50% of the molecule is made up of parallel polypeptide chains. If the chains were only approximately parallel they might account for a larger proportion of the molecule. The changes in the low-order reflexions produced by variation in the electron density of the suspension medium have been used to find the position of the molecules in the unit cell; confirmation of the results is found in the Patterson projections. The orientation of the haem group relative to the crystal axes is derived from measurements of paramagnetic resonance (Bennett & Ingram 1956 a ) and of optical dichroism. It is concluded that the plane of the haem group is inclined at 41° to the mean chain direction. Unambiguous information about the structure of the molecule will only be obtained by the isomorphous replacement method; meanwhile the above results, taken in conjunction with others derived from different crystal forms of myoglobin, have been used to discuss some plausible models. The most favoured is 42 Å long (in the chain direction) and has a cross-section of 25 x 35 Å. ... |
format |
Article in Journal/Newspaper |
title |
The crystal structure of myoglobin II. Finback whale myoglobin |
spellingShingle |
The crystal structure of myoglobin II. Finback whale myoglobin |
title_short |
The crystal structure of myoglobin II. Finback whale myoglobin |
title_full |
The crystal structure of myoglobin II. Finback whale myoglobin |
title_fullStr |
The crystal structure of myoglobin II. Finback whale myoglobin |
title_full_unstemmed |
The crystal structure of myoglobin II. Finback whale myoglobin |
title_sort |
crystal structure of myoglobin ii. finback whale myoglobin |
publisher |
The Royal Society |
publishDate |
1956 |
url |
http://dx.doi.org/10.1098/rspa.1956.0176 https://royalsocietypublishing.org/doi/pdf/10.1098/rspa.1956.0176 |
genre |
Balaenoptera physalus |
genre_facet |
Balaenoptera physalus |
op_source |
Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences volume 237, issue 1209, page 255-276 ISSN 0080-4630 2053-9169 |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rspa.1956.0176 |
container_title |
Proceedings of the Royal Society of London. Series A. Mathematical and Physical Sciences |
container_volume |
237 |
container_issue |
1209 |
container_start_page |
255 |
op_container_end_page |
276 |
_version_ |
1800748622311063552 |