Water, water everywhere: environmental DNA can unlock population structure in elusive marine species
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ),...
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crroyalsociety:10.1098/rsos.180537 2024-06-02T08:07:47+00:00 Water, water everywhere: environmental DNA can unlock population structure in elusive marine species Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda NOAA Office of Protected Resources NOAA Fisheries, NMFS 2018 http://dx.doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 en eng The Royal Society https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ Royal Society Open Science volume 5, issue 8, page 180537 ISSN 2054-5703 journal-article 2018 crroyalsociety https://doi.org/10.1098/rsos.180537 2024-05-07T14:16:46Z Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. Article in Journal/Newspaper Harbour porpoise Phocoena phocoena Alaska The Royal Society Royal Society Open Science 5 8 180537 |
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English |
description |
Determining management units for natural populations is critical for effective conservation and management. However, collecting the requisite tissue samples for population genetic analyses remains the primary limiting factor for a number of marine species. The harbour porpoise ( Phocoena phocoena ), one of the smallest cetaceans in the Northern Hemisphere, is a primary example. These elusive, highly mobile small animals confound traditional approaches of collecting tissue samples for genetic analyses, yet their nearshore habitat makes them highly vulnerable to fisheries by-catch and the effects of habitat degradation. By exploiting the naturally shed cellular material in seawater and the power of next-generation sequencing, we develop a novel approach for generating population-specific mitochondrial sequence data from environmental DNA (eDNA) using surface seawater samples. Indications of significant genetic differentiation within a currently recognized management stock highlights the need for dedicated eDNA sampling throughout the population's range in southeast Alaska. This indirect sampling tactic for characterizing stock structure of small and endangered marine mammals has the potential to revolutionize population assessment for otherwise inaccessible marine taxa. |
author2 |
NOAA Office of Protected Resources NOAA Fisheries, NMFS |
format |
Article in Journal/Newspaper |
author |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
spellingShingle |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
author_facet |
Parsons, Kim M. Everett, Meredith Dahlheim, Marilyn Park, Linda |
author_sort |
Parsons, Kim M. |
title |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_short |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_fullStr |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_full_unstemmed |
Water, water everywhere: environmental DNA can unlock population structure in elusive marine species |
title_sort |
water, water everywhere: environmental dna can unlock population structure in elusive marine species |
publisher |
The Royal Society |
publishDate |
2018 |
url |
http://dx.doi.org/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.180537 https://royalsocietypublishing.org/doi/full-xml/10.1098/rsos.180537 |
genre |
Harbour porpoise Phocoena phocoena Alaska |
genre_facet |
Harbour porpoise Phocoena phocoena Alaska |
op_source |
Royal Society Open Science volume 5, issue 8, page 180537 ISSN 2054-5703 |
op_rights |
https://royalsociety.org/journals/ethics-policies/data-sharing-mining/ |
op_doi |
https://doi.org/10.1098/rsos.180537 |
container_title |
Royal Society Open Science |
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5 |
container_issue |
8 |
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180537 |
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1800752907410210816 |